• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Prenatal DNA diagnosis by maternal blood

Research Project

Project/Area Number 07457610
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionShowa University

Principal Investigator

YANAIHARA Takumi  Showa University, School of Medicine, Professor, 医学部, 教授 (70102308)

Co-Investigator(Kenkyū-buntansha) HIROSE Kazuhiro  Showa University, School of Medicine, assistant, 医学部, 助手 (60261187)
SUZUKI Akira  Showa University, School of Medicine, lecturer, 医学部, 講師 (10221318)
SAITO Hiroshi  Showa University, School of Medicine, assistant Professor, 医学部, 助教授 (30119193)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Keywordsprenatal diagnosis / maternal blood / fetal DNA diagnosis / 有核赤血球 / 出生前遺伝子診断
Research Abstract

Recently it has known that nucleated fetal cells are found in maternal blood. Until now, prenatal diagnosis from fetal cells is performed invasively. An attempt was made to develop prenatal DNA diagnosis using fetal nucleated erythrocytes (NRBCs) from maternal blood. NRBCs from maternal blood (8-13 weeks of gestation) were obtained by percoll using discontinous density gradient method and cells were collected indivisually by micromanipulator. Genomic DNA was amplified by PEP-PCR method. We have established the identification method whether NRBCs are derived from fetus or not by HLA-DQalpha typing. This method will be applicable for female fetuses. We have published prenatal diagnosis for Duchenne muscular dystrophy, ornitine decarbamylase deficiency and fetal Rh blood type using this method. Furthermore, this method of prenatal diagnosis can be applied to other genomic disorders and is expected to become preffered method of noninvasive prenatal diagnosis of DNA.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 関沢 明彦: "妊娠末梢血を用いた出生前遺伝子診断" 産婦人科治療. 72 (1). 106-111 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 渡辺 顕: "母体末梢血中の胎児由来有核赤血球を用いた出生前診断の新たな試み-オルニチントランスカルバミラーゼ欠損症の出生前診断への応用-" 日本産科婦人科学会雑誌. 49 (9). 743-744 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] AKIHIKO SEKIZAWA: "Prenatol Diagnosis of the Fetal RhD Blood Type Using a Single Fetal Nucleated Erythrocyte From Maternal BlooD" Obstetrics & Gynecology. 87 (4). 501-501 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A. Sekizawa: "Prenatol diagnosis of Duchenne muscular dystrophy using a single fetal nucleated erythrocyte in maternal blood" American Academy of Neurology. 46. 1350-1353 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akihiko Sekizawa: "Analysis of HLA-DQ alpha sequences for prenatal diagnosis in single fetal cells from maternal blood" Human Genetics. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akihiko Sekizawa, Takehiko Kimura, Takashi Matsuoka, Hiroyuki Kurihara, Akira Watanabe, Hiroshi Saito, Takumi Yanaihara, Takeshi Sato: "Prenatal diagnosis using fetal nucleated erythrocytes from maternal blood." Obstetrical and Gynecolo. 72(in Japan). 106-111 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akihiko Sekizawa, Akira Watanabe, Takehiko Kimura, Takeshi Sato, Hiroshi Saito, Takumi Yanaihara: "Prenatal diagnosis of the RhD blood type using a single nucleated erythrocyte in maternal blood." Obstetrics and Gynecology. 87. 501-501 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akira Watanabe, Akihiko Sekizawa, Takehiko Kimura, Takeshi Sato, Hiroshi Saito, Takumi Yanaihara: "Noninvasive prenatal diagnosis of the RhD blood type using a single nucleated erythrocyte in maternal biood." Journal of Japanese O Nuturition and Metabo.2. 66 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akira Watanabe, Akihiko Sekizawa, Atsushi Taguchi, Hiroshi Saito, Takumi Yanaihara: "Prenatal diagnosis using a single uncleated erythrocyte (NRBC) from maternal blood : Clinical application for Ornithine transcarbamylase deficiency." Acta Obst Gynec Jon.(1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akihiko Sekizawa, Atsushi Taguchi, Akira Watanabe, Takehiko Kimura, Hiroshi saito, Takumi Yanaihara, Takeshi Sato: "Analysis of HLA-DQalpha sequences for prenatal diagnosis in single fetal cells from maternal blood." Human Genetics. (In Prss). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 矢内原巧他: "妊婦末梢血中有核赤血球を用いた胎児出生前診断法" 産婦人科の治療. 72. 106-111 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Yanaihara et al.: "Prenatal diagnosis of the RhD blood type using a single nucleated erythrocyte in maternal blood" Obstetrics and Gynecology. 87. 501-505 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 矢内原 巧・他: "妊娠末梢血を用いた出生前遺伝子診断" 産婦人科治療. 72. 106-111 (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi