| Project/Area Number |
07457618
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kitasato University School of Allied Health Sciences |
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Principal Investigator |
ITOH Hajime Kitasato University School of Allied Health Sciences, Department of Clinical Chemistry, Professor, 医療衛生学部, 教授 (10104564)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Zensuke Kitasato University School of Allied Health Sciences, Department of Clinical Che, 医療衛生学部, 助教授 (50152385)
SARUTA Takao Keio University School of Medicine, Department of Internal Medicine, Professor, 医学部, 教授 (70051571)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | essential hypertention / the third factor / digitalis-like substance / Na^+, K^+-ATPase |
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| Research Abstract |
A sepecific inhibitor of sodium-potassium-activatedadenosine 5'-triphosphatase (Na^+/K^+-ATPase) in urines from the patients with hyperpiesia was purified and the biochemical properties of the inhibitor was characterized. It was found that the inhibitor was directly purified from human urine with various HPLC systems including the column for sugar separation. The purified inhibitor was eluted as a single peak by ion-exchage chromatography in the sugar analytical system. The inhibitor in the peak fraction was thought to be an inducer of essential hypertension, because the time-dependent decrease of the specific peak was exactly correlated with its inhibitory activity. The inhibitory activity of the inhibitor was reduced in a time-dependent manner. Under the conditions, there were no detectable a specific peak of the inhibitor, but four peaks were observd when the compounds were generated from the inhibitor by spontaneous alteration.
This analytical system revealed the existence of sugars and amino groups in the inhibitor. Only a peak of these four compounds didn't passed through an amino-trap column. These results suggest that the Na^+/K^+-ATPase inhibitor has a sugar chain consisted of at least three sugars including amino groups, which may be responsible for the recognition of the specific receptor for Na^+/K^+-ATPase on the membrane of both smooth musle and adrenal basal cells.
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