| Project/Area Number |
07458150
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKASAKI Seiichi The University of Tokyo, Institute of Medical Science, Department of Biochemistry, Associated Professor, 医科学研究所, 助教授 (80112093)
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| Co-Investigator(Kenkyū-buntansha) |
KAWANO Takehiro The University of Tokyo, Institute of Medical Science, Department of Biochemistr, 医科学研究所, 助手 (30211866)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1995: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | lymphocytes / mucin-type sugar chain / O-glycan / N-acetylglucosamine / glycosyltransferase / Interleukin-4 / gene expression / colon carcinoma |
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| Research Abstract |
1. Regulation of oligosaccharide structures by core 2beta1,6 GlcNAc transferase (C2GnT) during lymphocyte activation : A human B lymphoblastic cell line, HuNS-1, was induced to express core 2 oligosaccharides by activation with IL-4. This induction was accompanied with increased expression of C2GnT activity and its mRNA.It is suggested, therefore, that the elevation of C2GnT may have some roles in the process of B cell activation.
2. Expression of sialy Lewis X accompanied with branch formation of sugar chains and its corelation with adhesiveness to endothelial cells : The gene coding the enzyme involved in synthesis of polysialic acids was introduced to CHO cells expressing sialy Lewis X.This transfection resulted in the decreased expression of sialyl Lewis X and the reduced adhesion of the cells to E-selectin.
3. Cloning of core 4beta1,6 GlcNAc transferase : The cDNA library was constructed from poly (A) + RNA of colon carcinoma LS174 cells. Then, several positive clones were obtained by cross hybridization, using homologous regions of the two beta1,6 GlcNAc transferases involved in synthesis of I antigenic determinant and core 2 oligosaccharide.
4. Structure, function and biosynthesis of the sugar moieties expressed on the branched core : Cloning of CMP-NeuAc hydroxylase were successfully performed. The structures of sugar chains which may serve as ligands for T-cell adhesion molecule CD2 were characterized.
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