| Project/Area Number |
07458153
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
MIHARA Katsuyoshi Graduate School of Medical Science, Professor, 大学院・医学系研究科, 教授 (40029963)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥8,100,000 (Direct Cost: ¥8,100,000)
Fiscal Year 1996: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1995: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | Precursor proteins / Mitochondria / Chaperones / Protein import / Membrane translocation / Hsp70 / ATPase / 前駆体 / タンパク質輸送 |
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| Research Abstract |
We have reconstituted the initial steps of mitochondrial protein import with a purified precursor protein, a purified, ATP-dependent, cytosolic chaperone selective for mitochondrial precursors (mitochondrial import stimulating factor ; MSF), and either intact mitochondria or intact or solubilized mitochondrial outer membranes. We show that the precursor-MSF complex first binds to the Tom37/Tom70 subunits of the mitochondrial import receptor. After ATP-dependent release of MSF,the precursor is transferred from Tom37/Tom70 to the Tom20/Tom22 subunits of the receptor, and finally delivered to the import channel in the outer membrane. Import in the absence of the MSF bypasses Tom37/Tom70. The ATP-mediated transfer of a precursor from MSF to specific subunits of the import receptor is similar to the GTP-mediated transfer of precursors from the signal recognition particle to its receptor on the endoplasmic reticulum.
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