| Project/Area Number |
07458171
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
HAKOSHIMA Toshio Nara Institute of Science and Technology Department of Molecular Biology Professor, バイオサイエンス研究科, 教授 (00164773)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1996: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1995: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | transcription factor / basic transcription factor / protein / DNA / molecular complex / X-ray / structural biology / 大腸菌 / σ因子 / ピロモータ / 認識 |
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| Research Abstract |
Purification, characterization and crystallization of sigma factors, sigma28, sigma38, and sigma70, were performed. Measurements of fluorescent polarization and surface plasmon resonance showed that sigma28 and sigma38 could bind to promoter DNAs, especially strongly to the-10 region of the promoters. Crystals of sigma38 were obtained by a vapor-diffusion method. Crystal structure of a yeast transcription factor PHO4 complexed with DNA was solved by the conventional MIR method. The crystal structure revealed how PHO4 recognizes the DNA sequence at an atomic resolution. Crystals of a murine transcription factor IRF-2 complexed with DNA and a yeast transcription factor papl bound to DNA also were obtained. Structure determination of these crystals are underway. Crystal structure of a HPt (histidine-containing phosphotransfer) domain of E.coli sensor protein ArcB was solved. The crystal structure indicated that the domain exists both in prokaryote and eukaryote.
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