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Genetic Recombination and the Function of RNA during Meiosis

Research Project

Project/Area Number 07458183
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

HOTTA Yasuo  School of Bioscience, Nara institute of Science and Technology, Professor, バイオサイエンス研究科, 教授 (30190218)

Co-Investigator(Kenkyū-buntansha) TAKASE Hisafumi  School of Bioscience, Nara institute of Science and Technology, Joshu, バイオサイエンス研究科, 助手 (20263444)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥8,100,000 (Direct Cost: ¥8,100,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1995: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsMeiosis / Recombination / Pollen / LIM14 / LIM15 / Tapetal cell / Plastid / Starch Granule / 減数分裂特異的遺伝子 / 組換え / RecA / 転写調節 / プロモーター解析 / 減数分裂特異的RNA / LIM15遺伝子 / 減数分裂特異的プロモーター / Zyg DNA / Psn DNA / 減数分裂特異的蛋白質 / トポイソメラーゼと染色体
Research Abstract

We have 18 cloned DNAs expressed during first meiotic prophase of lily microsporocytes by the substraction hybridization against the somatic RNAs isolated from very young anther. We are trying to understand the function of these genes. First, we have obtained complete LIM13 DNA previously missing the 5'end and concluded the basic sequences of all 18 cDNAs. Secondly, LIM15 DNA showing a high homology with E.coli RecA which is a important factor for homologous recpmbination in prokaryotes, has been localized on meiotic prophase chromosomes by using anibodies prepared against LIM15 protein synthesized in E.cili cells. LIM15 protein are found in many spots along the pairing and paired chromosome. The number of such spots are far more than the number chiasma (order of X10^3) and can be agreeable with the DNA repair spots reported earlier. It has been found that RAD52 and other RAD proteins co-localize with LIM15 and we consider this as the suggestive evidence that recombination requires than one protein.
On the other hand, LIM14 gene is expressed in meiotic cells but the product has been detected in tapetal cells, tetrads and in pollen till the maturity. LIM14 protein is localized in the staarch granules of those cells but not in the starch granules in stem, leaf, root and the anther wall cells. The LIM14 protein is rich in glycine and serine contents. It has some similarity in the amino acid sequence with the reported cell wall protein. LIM14 protein has a plastid-transport signal at the N-terminal peptide, which can be useful if one trys to send a protein to the plastids or starch granules.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Hotta, Y.: "Meiosis specific transcription and functional proteins." Adv.Biophys.31. 101-115 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Terasawa, M.: "Localization of RecA-like recombination proteins on chromosomes of lily at various meiotic stages." Genes and Develop.9. 925-934 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 平塚 和之: "雄性配偶体形成過程の分子解剖。" 育種学・最近の進歩. 38. 47-50 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高瀬 尚文: "植物のDNA組換えシステム。" 化学と生物. 34. 683-691 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hotta, Y.: "Regulation of genes expressed during meiosis." Analysis and Utility of Plant Chromosome Information.2-8 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsumennasan, Kh.: "Investigations in the F1 hybrid and backcross progeny of cattle (Bos taurus) and yak (Bos grunniens) in Mongolia." Cytogenetics and Cell Genetics. 78. 69-73 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hotta, Y., Furukawa, K.and Tabata, S.: "Meiosis specific transcription and functional proteins." Adv.Biophys.31. 101-115 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Terasawa, M., Shinohara, A., Hotta, Y., Ogawa, H., and Ogawa, T.: "Localization of RecA-like recombination proteins on chromosomes of lily at various meiotic stages." Genes and Develop.9. 925-934 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiratsuka, K., Takase, H.and Hotta, Y.: "Molecular anatomy of male gametophyte development. (Japanese)" Recent Advancement in Breeding. 38. 47-50 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takase, H., Hiratsuka, K.and Hotta, Y.: "DNA recombination system in plants. (Japanese)" Chemistry and Biology. 34. 683-691 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hotta, Y., Takase, H.and Hiratsuka, K.: "Regulation of genes expressed during meiosis. (Plenary lecture)" Analysis and utility of plant chromosome information.2-8 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsumennasan, Kh., Tserenbat, T., Hotta, Y., Takase, H., Speed, T.M.and Chandley, A.: "Investigations in the Fl hybrid and bckcross progeny of cattle (Bos taurus) and yak (Bos gruniens) in Mongolia." Cytogenetics and Cell Genet.78. 69-73 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takase, H.and Hotta, Y.: "Meiosis and DNA replication. (Japanese)" Plant Molecular Biology ed.by Yamada, Y.49-59 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hotta,Y.: "Meiosis specific transcription and functional proteins." Adv.Biophys.31. 101-115 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Terasawa,M.: "Localization of RocA-like recombination proteins on chromosomes of lily at various meiotic stages." Genes and Develop.9. 925-934 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] 平塚和之: "雄性配偶体形成過程の分子解剖。" 育種学・最近の進歩. 38. 47-50 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高瀬尚文: "植物のDNA組換えシステム。" 化学と生物. 34. 683-691 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hotta,Y.: "Regulation of genes expressed during meiosis." Analysis and Utility of Plant Chromosome Information. 2-8 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsumennasan,Kh.: "Investipations in the F1 hybrid and backcross progeny of cattle (Bos taurus) and yak (Bos grunniens) in Mongolia." Cytogenetics and Cell Genetics. 78. 69-73 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高瀬尚文: "植物のDNA組換えシステム:遺伝的組換えとその制御" 化学と生物. 34. 683-691 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 平塚和之: "雄性配偶体形成過程の分子解剖" 育種学最近の進歩. 38. 47-50 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Terasawa,M.et al.: "Localization of RecA-like recombination proteins on ckromosome of lily at rarious meiotic stages." Genes & Develop.9. 925-934 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato,S.et al.: "Structural analysis of recA-like gene in the genome of Arabidopsis thaliana." DNA Research. 2. 89-93 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato,S.et al.: "Expression profiles of a human gene ideatified as a structural homologue of meiosis-specific reA-like genes." DNA Research. 2. 183-186 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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