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The role of substrate-specific molecular chaperone in the trimerization of procollagen.

Research Project

Project/Area Number 07458190
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NAGATA Kazuhiro  Kyoto University, Chest Disease Resenrch Institute, Department of Cell Biology, Professor, 胸部疾患研究所, 教授 (50127114)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1996: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordsstress protein / molecular chapreone / collagen / HSP47 / プロコラーゲン
Research Abstract

HSP47 was originally identified as a collagen-specific stress protein located in the endoplasmic reticulum (ER). HSP47 binds to procollagen in the ER immediately after the nascent chain of procollagen enters the ER and dissociates from it in the cis-Golgi network. Binding affinity of HSP47 to various types of collagens including types I to V was revealed to be similar using BIAcore biosensor. The expression of HSP47 closely correlates with that of collagens including types I to IV in various cell lines and during the development of mouse embryos. Both HSP47 and types I and III collagens were also induced in some pathological conditions such as during the progression of liver fibrosis caused by the administration of carbon tetrachrolide into rats.
We showed the results of the transfection of antisense RNA for HSP47 into BALBc/3T3 cells. We obtained several stable transfectants where the synthesis and accumulation of HSP47 were inhibited moderately and almost completely. The expression of procollagen was observed to be inhibited at levels of both protein synthesis and mRNA accumulation in the cells containing low level of HSP47. In addition to the inhibition of collagen synthesis, the secretion of procollagen was inhibited in these cells although the inhibition was not so evident because of the low level of collagen synthesis. Next, we tried to transfect the cDNA encoding alpha1 chain of type I collagen into the HSP47-antisense transfected cells. In this double transfectants, the level of HSP47 was low while the amount of procollagen alpha1 chain was comparable with that of control cells. In these cells, we found that procollagen was recovered in the detergent-insoluble fraction, indicating that HSP47 is involved in the solubility of alpha chains of pprocollagen in the ER.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] K. NAGATA: "HSP47 : a collagen-specific molecular chaperone in the endoplasmic reticulum." TiBS. (Trends in Biochemical Science). 21(1). 23-26 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. S. H. EL-THAHER: "The pH-dependent, ATP-independent interaction of collagen specific serpin/stress protein HSP47." Prot Pept Lett.3(1). 1-8 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. SATOH: "Intracellular interaction of collagen-specific stress protein HSP47 with newly synthesized procollagen." J. Cell Biol.133(2). 469-483 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J. NISHIZAWA: "Reperfusion causes significant activation of heat shock transcription factor 1 in ischemic rat heart." Circulation. 97(9). 2185-2192 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K. NAGATA: "Regulation and function of collagen-specific molecular chaperone, HSP47." Cell Structure and Function. (Dynamics of the Cell). 21(5). 425-430 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A. NAKAI: "HSF4, a new member of the human heat shock factor gene family which lacks properties of a transcriptional activator." Mol. Cell. Biol.17(1). 469-481 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.NAGATA: "HSP47 : a collagen-specific molecular chaperone in the endoplasmic reticulum" TiBS (Trends in Biochemical Sciences). 21(1). 23-26 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.S.H.EL-THAHER: "The pH-dependent, ATP-independent interaction of collagen specific serpin/stress protein HSP47" Prot Pept Lett.3(1). 1-8 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.SATOH: "Intracellular interaction of collagen-specific stress protein HSP47 with newly synthesized procollagen." J.Cell Biol.133(2). 469-483 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.NISHIZAWA: "Reperfusion causes significant activation of heat shock transcription factor 1 in ischemic rat heart" Circulation. 94(9). 2185-2192 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.NAGATA: "Regulation and function of collagen-specific molecular chaperone, HSP47" Cell Structure and Function. (Dynamics of the Cell). 21(5). 425-430 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.NAKAI: "HSF4, a new member of the human heat shock factor gene fmily which lacks properties of a transcriptional activator." Mol.Cell Biol.17(1). 469-481 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.NAGATA: "HSP47 : a collagen-specific molecular chaperone in the endoplasmic reticulum." TiBS.(Trends in Biochemical Sciences). 21(1). 23-26 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.S.H.EL-THAHER: "The pH-dependent,ATP-independent interaction of collagen specific serpin/stress protein HSP47." Prot Pept Lett.3(1). 1-8 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.SATOH: "Intracellular interaction of collagen-specific stress protein HSP47 with newly synthesized procollagen." J.Cell Biol.133(2). 469-483 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] J.NISHIZAWA: "Reperfusion causes significant activation of heat shock transcription factor 1 in ischemic rat heart." Circulation.94(9). 2185-2192 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.NAGATA: "Regulation and function of collagen-specific molecular chaperone,HSP47." Cell Structure and Function.(Dynamics of the Cell). 21(5). 425-430 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.NAKAI: "HSF4,a new member of the human heat shock factor gene family which lacks properties of a transcriptional activator." Mol.Cell.Biol.17(1). 469-481 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] N. NAGAI: "Quercetin suppresses heat shock response by down regulation of HSP1." Biochem. Biophys. Res. Commun.208. 1099-1105 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. HIGASHI: "Activation of heat shock factor 1 in rat brain during cerebral ischemia or after heat shock." Mol. Brain Res.34. 262-270 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A. NAKAI: "The DNA-Binding properties of two heat shock factors,HSF1 and HSF3, are induced in the avian erythroblast cell line HD6." Mol. Cell. Biol.15. 5268-5278 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. YASUDA: "Cloning and expression of murine high-molecular-wight heat shock proteins, HSP105." J. Biol. Chem.270. 29718-92723 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.NAGATA: "HSP47: a collagen-specific morecular chaperone in the endoplasmic reticulum." TiBS.(trends in Biochemical Sciences). 21. 23-26 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] M. SATOH: "Intracellular interaction of collagen-specific stress protein HSP47 with newly synthesized procollagen." J. Cell Biology. (in press.). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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