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Analysis of the comprising membrane-anchored growth factor and cell adhesion molecule.

Research Project

Project/Area Number 07458192
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionKurume University

Principal Investigator

MEKADA Eisuke  Institute of Life Sciense, Kurume University, Professor, 分子生命科学研究所, 教授 (20135742)

Co-Investigator(Kenkyū-buntansha) UMATA Toshiyuki  Institute of Life Sciense, Kurume University, Assistant Professor, 分子生命科学研究所, 助手 (30213482)
IWAMOTO Ryo  Institute of Life Sciense, Kurume University, Assistant Professor, 分子生命科学研究所, 助手 (10213323)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1996: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1995: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordsjuxtacrine / integrin / HB-EGF / CD9 / TM4 / heparansulfate proteoglycans / membrane-anchored growth factors / DRAP27 / CD63 / ジフテリア毒素リセプター / TPA
Research Abstract

We have found that the membrane-anchored form of heparin-binding EGF-like growth factor (proHB-EGF) forms a complex with CD9 and integrin alpha3bl. The final goal of this study is to understand the physiological role of this complex and the mode of action of juxtacrine growth factors. we have done several experiments and obtained the following results.
(1) Localization of the complex
Vero cell, derived from monkey kidney, was studied by immunofluorescence using specific antibodies to proHB-EGF,CD9, integrin alpha3, and integrin beta1. The results revealed that proHB-EGF,CD9 and integrin alpha3b1 localized at cell-cell junction region.
(2) The role of heparin-binding domain (HBD)
To analyze the role of HBD domain of proHB-EGF,the deletion mutants of HBD of proHB-EGF were produced. The results showed that HBD is not essential for the mitogenic activity and diphtheria toxin binding activity.
(3) Expression of CD9 in myelin
Immunohistochemical studies showed that CD9 is expressed in myelin of human central and peripheral nervous systems. The expression of ProHB-EGF was not detected in myelin.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Tsuneoka,M.: "c-myc activates RCC1 gene expression through E-box elements." Oncogene. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakagawa,T.: "Amino-terminal processing of cell surface heparin-binding EGF-like growth factor upregulates its juxtacrine but not its paracrine growth factor activity." J. Biol. Chem.271. 30858-30863 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura,Y.: "Expression and distribution of CD9 in myelin of the central and peripheral nervous systems." Am. J. Pathol.149. 575-583 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuneoka, M.: "c-myc activates RCC1 gene expression through E-box elements." Oncogene.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakagawa, T.: "Amino-terminal processing of cell surface heparin-binding EGF-like growth factor upregulates its juxtacrine but not its paracrine growth factor activity." J.Biol.Chem.271. 30858-30863 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura, Y.: "Expression and distrubution of CD9 in myelin of the central and peripheral nervous systems." AM.J.Pathol.149. 575-583 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mitamura.T.: "Diphtheria toxin binds to the EGF-like domain of human heparin-binding EGF-like growth factor/diphtheria toxin receptor and inhibits specifically its mitogenic activity." J.Biol.Chem.270. 1015-1019 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Higashiyama, S.: "The membrane protein CD9/DRAP27 potentiates the growth factor activity of the membrane-anchored heparin-binding EGF-like growth factor (HB-EGF)." J.Cell.Biol. 128. 929-938 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura, K.: "Membrane anchored heparin-binding EGF-like growth factor and DRAP27/CD9 form a complex with integrin 3 l at cell-cell contact sites." J.Cell.Biol. 129. 1691-1705 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Goishi, K.: "Phorbol ester induces the rapid processing of cell surface heparin-binding EGF-like growth factor ; Conversion from juxtacrine to paracrine growth factor activity." Mol.Biol, Cell. 6. 967-980 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shishido, Y.: "Heparin-like molecules on the cell surface potentiate binding of diphtheria toxin to the diphtheria toxin receptor/membrane-anchored heparin-binding EGF-like growth factor." J.Biol.C. 270. 29578-29585 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mekada, E.: Volume8 Bacterial toxins and virulence factors in desease. Moss, J., Iglewski, B., Vaughan, M.& Tu, A.T.(Marcel Dekker Inc., New York)Handbook of natural toxins, 15 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuneoka,M.: "c-myc activates RCC1 gene expression through E-box elements." Oncogene. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakagawa,T.: "Amino-terminal processing of cell surface heparin-binding EGF-like growth factor upregulates its juxtacrine but not its paracrine growth factor activity." J.Biol.Chem.271. 30858-30863 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakamura,Y.: "Expression and distribution of CD9 in myelin of the central and peripheral nervous systems." Am.J.Pathol.149. 575-583 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mitamura, T.: "Diphtheria toxin binds to the EGF-like domain of human heparin-binding EGF-like growth factor/diphtheria toxin receptor and inhibits specifically its mitogenic activity." J. Biol. Chem.270. 1015-1019 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Higashiyama, S.: "The membrane protein CD9/DRAP27 potentiates the growth factor activity of the membrane-anchored heparin-binding EGF-like growth facor (HB-EGF)." J. Cell Biol.128. 929-938 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakamura, K.: "Membrane anchored heparin-binding EGF-like growth factor and DRAP27/CD9 form a complex with integrin 3 l at cell-cell contact sites." J. Cell Biol.129. 1691-1705 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Goishi, K.: "Phorbol ester induces the rapid processing of cell surface heparin-binding EGF-like growth factor; Conversion from juxtacrine to paracrine growth factor activity." Mol. Biol. Cell. 6. 967-980 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shishido, Y.: "Heparin-like molecules on the cell surface potentiate binding of diphtheria toxin to the diphtheria toxin receptor/membrane-anchored heparin-binding EGF-like growth factor." J. Biol. Chem.270. 29578-29585 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mekada, E.: "Handbook of natural toxins: Volume 8, Bacterial toxins and virulence factors in disease" Moss, J., Iglewski, B., Vaughan, M. & Tu, A. T. (Marcel Dekker Inc., New York), 15 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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