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STUDY ON TRANSCRIPTION FACTORS REGULATING GENE EXPRESSION IN SEA URCHIN EMBRYOS

Research Project

Project/Area Number 07458195
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

SHIMADA Hiraku  HIROSHIMA UNIVERSITY,FACULTY OF SCIENCE,PROFESSOR, 理学部, 教授 (70011559)

Co-Investigator(Kenkyū-buntansha) NAKATSUBO Keiko  HIROSHIMA UNIVERSITY,FACULTY OF SCIENCE,INSTRUCTOR, 理学部, 助手 (40192760)
AKASAKA Koji  HIROSHIMA UNIVERSITY,FACULTY OF SCIENCE,ASSOCIATE PROFESSOR, 理学部, 助教授 (60150968)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1996: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1995: ¥4,300,000 (Direct Cost: ¥4,300,000)
Keywordssea urchin / arylsulfatase / gene expression / embryo / enhancer / 0tx / insulator / 発生 / 転写因子 / ミトコンドリア / リボソームRNA / Ars遺伝子 / 転写調節 / Gストリング
Research Abstract

The present research project has been undertaken to elucidate a cascade of transcription factors related to temporally and spatially regulated expression of genes during early development of sea urchin. Results obtained during the research period for this grant are as follows.
(1) A procedure to introduce exogenous DNA into sea urchin eggs by a particle gun has been developed.
(2) Temporal expression of the Ars gene is regulated by two distinct cisactive elements in its promoter region (from -252 to +38bp), though level of transcription initiated by these elements alone is very low and declines rapidly.
(3) GGATTA sequences (known as binding sites for transcription factor 0tx) in the first intron of the Ars gene act as a transcriptional enhancer of this gene. Two distinct cDNAs (Hp0tx_E and Hp0tx_L) encoding Hp0tx have been cloned. Hp0tx_E is a maternal factor and suppresses the Ars gene transcription, while Hp0tx_L is produced in hatched blastulae and activates the Ars gene transcription. Two cDNAs are produced from the same gene by alternative splicing.
(4) It is found that the region from -2686 to -2113bp of the Ars gene acts as an transcriptional insulator that prevents the Ars gene to be transcribed under the influence of promoter of the gene located in a closely vicinity.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Sakamoto et al.: "A triplex DNA configuration of the polypyrimldine : polypurine stretch in the 5' flanking region of the sea urchin arylsulfatase gene." Zoological Science. 13. 105-109 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Harada et al: "Spatial expression of a forkhead homologue in the sea urchin embryo." Mechanism of Development. 60. 163-173 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mitsunaga-Nakatsubo et al: "cDNA cloning of Na_+,K_+-ATPase alpha subunit from embryos of the sea urchin,Hemicentrotus pulcherrimus." Zoological Science. 13. 833-841 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakamoto et al: "Two isoforms of orthodenticle-related proteins (HpOtx) bind to the enhancer element of sea urchin arylsulfatase gene." Developmental Biology. 181. 284-295 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akasaka et al: "Oral-aboral ectoderm differentiation of the sea urchin embryos is disrupted in response to calcium ionophore." Development Growth and Differentiation. 39(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morokuma et al: "A cis-regulatory element within the 5' flanking region of arylsulfatase gene of sea urchin,Hemicentrotus pulcherrimus" Development Growth and Differentiation. 39(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Sakamoto, K.Akasaka, T.Yamamoto and H.Shimada: "A triplex DNA configuration of the polypyrimidine : polypurine stretch in the 5' flanking region of the sea urchin arylsulfatase gene." Zool.Sci.13. 105-109 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Harada, K.Akasaka, H.Shimada, K.J.Peterson, E.H.Davidson and N.Satoh: "Spatial expression of a forkheao homologue in the sea urchin embryo." Mech.Dev.60. 163-173 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Mitsunaga-Nakatsubo, K.Yamazaki, M.Hatoh-Okazaki, H.Kawashita, C.Okamura, K.Akasaka, H.Shimada and I.Yasumasu: "cDNA cloning of Na+, K+-ATPase alpha subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus." Zool.Sci.13. 833-841 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Sakamoto, K.Akasaka, K.Mitsunaga-Nakatsubo, K.Takata, T.Nishitani and H.Shimada: "Two isoforms of orthodenticlerelated proteins (Hp0tx) bind to the enhancer element of sea urchin arylsulfatase gene." Dev.Biol.181. 284-295 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Akasaka, H.Uemoto, F.Wilt, K.Mitsunaga-Nakatsubo and H.Shimada: "Oral-aboral ectoderm differentiation of the sea urchin embryos is disrupted in response to calcium ionophore." Develop.Growth & Differ. 39(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Morokuma, K.Akasaka, K.Mitsunaga-Nakatsubo and H.Shimada: "A cis-regulatory element within the 5' flanking region of arylsulfatase gene of sea urchin, Hemicentrotus pulcherrimus." Develop.Growth & Differ. 39(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakamoto et al.: "A triple DNA configuration of the polypyimidine : polypurine stretch in the 5' flanking region of th sea urchin arylsulfatase gene." Zoological Science. 13. 105-109 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Harada et al: "Spatial expression of a forkhead homologue in the sea urchin embryo." Mechanism of Development. 60. 163-173 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mitsunaga-Nakatsubo et al: "cDNA cloning of Na+, K+-ATPase alpha subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus." Zoological Science. 13. 833-841 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakamoto et al: "Two isoforms of orthodenticle-related proteins (HpOtx) bind to the enhancer element of sea urchin arylsulfatase gene." Developmental Biology. 181. 284-295 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akasaka et al: "Oral-aboral ectoderm differentiation of the sea urchin embryos is disrupted in response to calcium ionophore." Development Growth and Defferentiation. 39 (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Morokuma et al: "A cis-regulatory element within the 5' flanking region of arylsulfatase gene of sea urchin, Hemicentrotus pulcherrimus" Development Growth and Differentiation. 39 (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Iuchi et al: "Detection and characterization of the cis-element in the first intro n of the Ars gene in sea urchin embryo" Development, Growth & Differentiation. 37. 373-378 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Yamasu et al: "Molecular cloning of a cDNA that encodes the precursor to several gastrula-inducing peptides, EGF-related peptides of the sea urchin" European Journal of Biochemistry. 228. 515-523 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Akasaka et al: "Introduction of DNA into sea urchin eggs by particle gun" Molecular Marine Biology and Biotechnology. 4. 255-261 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] R. Murakami et al: "aproctous, a locus that is necessary for the development of the proctodeumin Drosophila embryos, encodes a homolog of the vertebrate Brachyury gene." Roux′s Archives of Developmental Biology. 205. 89-96 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] N. Sakamoto et al: "Atriplex DNA configuration of the polypyrimidine: polypurine stretch in the 5′ flanking region of the sea urchin arylsulfatase gene." Zoological Science. 13. 105-109 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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