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A comparative study of somatic and visceral afferent fibers in pain processing mechanism.

Research Project

Project/Area Number 07458199
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionFukushima Medical College

Principal Investigator

SUGIURA Yasuo  Fukushima Medical College, Department of Anatomy, Associate Professor, 医学部, 教授 (50093042)

Co-Investigator(Kenkyū-buntansha) OZAKI Noriyuki  Fukushima Medical College, Department of Anatomy, Research Associate, 医学部, 助手 (40244371)
HONDA Takashi  Fukushima Medical College, Department of Anatomy, Research Associate, 医学部, 助手 (20165608)
TONOSAKI Yoshikazu  Fukushima Medical College, Department of Anatomy, Research Associate, 医学部, 助手 (50155545)
NISHIYAMA Keiji  Fukushima Medical College, Department of Anatomy, Associate Professor, 医学部, 助教授 (10106354)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsVisceral afferent / Somatic afferent / WGA-HRP / Spinal afferent / Vagal afferent / Stomach / Fos protein / Dura mater / 侵害受容機構 / 体性感覚 / 内臓感覚 / c-fos / ストレス / 脊髄硬膜
Research Abstract

The visceral and somatic sensory innervation of the gastric pylorus and the spinal dura mater were investigated by anterograde, retrograde tracing and immunohistochemical methods. Primary afferent neurons were investigated by injecting wheat germ agglutinin-conjugated horseradish peroxidase into the pylorus. Labeled neurons were observed bilaterally within the vagal and spinal ganglia. The vagal neurons innervating the pyloric antrum were smaller than the spinal neurons. Peripheral afferent terminals in the pyloric antrum arising from the vagal or the spinal nerves were anterogradely labeled by injection of Dil into the vagal or T9 dorsal root ganglia, respectively. Both vagal and spinal afferent terminals were particularly abundant in the mucosa, and to a lesser extent in the muscular layr. Vagal afferent fibers in the mucosa were found at the base of the pyloric glands and spinal afferents ascended toward the surface epithelium through the lamina propria. The vagal afferent terminals were CGRP positive.
Fos expression was investgated in the spinal cord after stimuli around the stomach. Peritoneal stimulation by acetic acid elicited Fos protein in the medulla and the spinal cord, but inner application of a formalin solution in the stomach failed to express the spinal Fos protein. In stress condition, however, Fos expression was found in the spinal cord by the same stimuli. The fibers contributed to express Fos protein may be so-called silent fibers.
The density of CGRP-immunoreative fibers in the lower lumbar dura mater was reduced to 45.5% of the normal rats by L2-3 lumber sympathectomy. The cervical dura mater, however, did not change after stellateganglioectomy and then the distribution of sensory or sympathetic fibers in the dura mater were varied at the level of the vertebrae.
The difference of fiber distribution in organs from vagal and spinal afferent neurons may reflect the functional correlation between the two types (visceral and somatic) of afferents.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Sekiguchi,Y.,Konnai,Y.,Kikuchi,S.,Sugiura,Y.: "An anatomic study of neuropeptide immunoreactivities in the lumbar dura mater after sympathectomy" Spine. 21. 925-930 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ozaki,N.,Sugiura,Y.,Yamamoto,M.,Nishiyama,Y.: "Induction of Fos protein expression in spinal cord neurons by herpes simplex virus infections in the mouse" Neuroscience Letters. 216. 61-64 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Honda,T.,Ozaki,N.,Tonosaki,Y.,Nishiyama,K.,Sugiura,Y.: "Neuronal damage of the primary afferent neurons evoke Fos expression even in the spinal dorsal horn of the capsaicin-induced analgesic rats" Pain Research. 10. 61-70 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Honda,T.,Ozaki,N.,Tonosaki,Y.,Nishiyama,K.,Shigemoto,R.,Sugiura,Y.: "Synaptic organization and ultrastructural features of the substance P-receptor like immunoreactive neurons in the nucleus intermediolateralis of rats" Neuroscience Letters. 197. 1-4 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tonosaki,Y.,Nishiyama,K.,Honda,T.,Ozaki,N.,Sugiura,Y.: "D-2 like dopamine receptor mediates dopaminaergic of γ-aminobutyric acidergic inhibition of mealnotropin-releasing hormone release from the par intermedia in frogs(Rana nigromaculata)" Endocrinology. 136. 5260-5265 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayashi,A.,Yoshida,N.,Tonosaki,Y.,Kikuchi,S.,Sugiura,Y.: "Origin of the calcitonin gene-related peptide-immunoreactive nerve fibers in the rat shoulder joint." Anatomy and Embryology. 191. 471-476 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugiura,Y.: "Spinal organization of C-fiber afferents related with nociception or non-nociception. In the Polymodal Receptor" Progress Brain Research,Elsevier, 547(319-339) (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugiura,Y.,Tonosaki,Y.: "Spinal organization of unmyelinated afferent fibers in comparison with somatic afferent fibers In Visceral pain" Progress in Pain Research and Management IASP Press, 516(41-59) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sekiguchi, Y., Konnai, Y., Kikuchi, S., Sugiura, Y.: "An anatomic study of neuropeptide immunoreactivities in the lumbar dura mater after sympathectomy." Spine 21. 925-930 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ozaki, N., Sugiura, Y., Yamamoto, M., Nishiyama, . Y.: "Induction of Fos protein expression in spinal cord neurons by herpes simplex virus infections in the mouse." Neurosciencxe Letters. 216. 61-64 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Honda, T., Ozaki, N., Tonosaki, Y., Nishiyam, K., Sugiura, Y.: "Neuronal damage of the primary afferent neurons evoke Fos expression even in the spinal dorsal horn of the capsaicin-induced analgesic rats." Pain Reseach. 10. 61-70 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Honda, T., Ozaki, N., Tonsaki, Y., Nishiyama, K., Shigemoto, Y., Sugiura, Y.: "Synaptic organization and ultrastructural features of the substance P-receptor like immunoreactive neurons in the nucleus intermediolateralis of rats." Neuroscience Letters. 197. 1-4, 309-319 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tonosaki, Y., Nishiyama, K., Honda, T., Ozaki, N., Sugiura, Y.: "D-2 like dopamine receptor mediates dopaminaergic of gamma-aminobutyric acidergic inhibition of mealnotropin-releasing hormone release from the par intermedia in frogs (Rana nigromaculata)." Endocrinology. 136. 5260-5265 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayasi, A., Yoshida, N., Tonosaki, Y., Kikuchi, S., Sugiura, Y.: "Origin of the calcitonin gene-related peptide-immunoreactive nerve fibers in the rat shoulder joint." Anatomy and Embryology. 191. 471-476 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yosidda, N., Nishiyama, K., Tonosaki, Y., Kikuchi, S., Sugiura, Y.: "Sympathetic and sensory innervaion of the rat shoulder joint : a WGA-HRP tracing and CGRP immunohistochemiscal study." Anatomy and Embryology. 191. 456-469 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugiura, Y.: "Spinal organization of C-fiber afferents related with nociception or non-nociception." Progress Brain Reseach. 112. 319-339 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugiura, Y., Tonosaki, Y.: "Spinal organization of unmyelinated afferent fibers in comparison with somatic afferent fibers. Visceral pain" Progress in Pain Research and Management. 5. 41-59 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 関口泰文,ら: "An anatomic study of neuropeptide immunoreactivities in the lumbar dura mater after sympathectomy." Spine. 21. 925-930 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 尾崎紀之,ら: "Induction of Fos protein expression in spinal cord neurons by herpes simplex virus infections in the mouse." Neuroscience Letters.,. 216. 61-64 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 本多たかし,ら: "Neuronal damage of the primary afferent neurons evoke Fos expresion even in the spinal dorsal horn of the capsaicin-induced analgesic rats." Pain Research.,. 10. 61-70 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 本多たかし,ら: "Synaptic organization and ultrastructural features of the substance P-receptor like immunoreactive neurons in the nucleus intermediolateralis of rats." Neuroscience Letters.,. 197. 1-4 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 外崎敬和,ら: "D2-like dopamine receptor mediates dopaminergic or r-aminobutyric acidergic inhibition of melanotropin-releasing hormone release from the pars intermedia in frogs(Rana nigromaculata)" Endoctinology.,. 16. 5260-5265 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岩間裕,ら: "Cervical sympathectomy affects gonadotropin-releasing hormone,intermizing hormone and testosterone in male rats." J.of Anesthesia. 9. 166-169 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 杉浦 康夫(分担): "Spinal organization of C-fiber afferents related with nociception or non-nociception in Prog,in Brain Research 112" Elsevier Pub co Ltd., 319-339 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 杉浦 康夫(分担): "C-fiber afferentsの電磁顕微鏡的観察(痛みの分子機構)" ニュー.サイエンス社, 462-465 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Honda,S.Oda,N.Ozaki,Y.Tonosaki,K.Nishiyama and Y.Sugiura: "Neuronal damage of the primary afferent neurons elicits Fos expression even in the spinal dornal horn of the capsaicin-induced analgesic rats" Pain Research. 10. 61-70 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Honda,N.Ozaki,Y.Tonosaki,K.Nishiyama,R.Shigemoto and Y.Sugiura: "Synaptic organization and ultrastructural features of the substance P-receptor-like immunoreactive neurons in the nucleus intermediolateralis of rats" Neuroscience Letters. 197. 117-120 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Sekiguchi,Y.Konnai,S.Kikuchi and Y.Sugiura: "An anatomic study of Neuropeptide immunoreactivities in the lumbar dara mater after lumbar sympathectomy" Spine. 21(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Sugiura and Y.Tonosaki: "Spinal organization of unmyelinated visceral afferent fibers in comparison with somatic afferent fibers" Visceral Pain,Progress in Pain Rsearch and Management,. 5. 41-59 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] N.Ozaki,K.Nishiyama,Y.Tonosaki,T.Honda,and Y.Sugiura: "Expression of Fos protein in the nucleus tractus solitarius and spinal cord after irritation of the stomach in the rat" Fourth IBRO World Congress of Neuroscience Abstracts. 307 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] N.Ozaki,K.Nishiyama,Y.Tonosaki,T.Honda,and Y.Sugiura: "Expression of Fos protein in the nucleus solitarius due to acute stomach ulcer induced by ethanol administration." Neuroscience Research supplement. 19. S217 (1994)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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