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Establishment of novel conditional gene targeting methods using Cre-loxP system

Research Project

Project/Area Number 07458216
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionTohoku University (1997)
Japanese Foundation For Cancer Research (1995-1996)

Principal Investigator

NODA Tetsuo  Tohoku University, Medicine, Professor, 医学部, 教授 (10183550)

Co-Investigator(Kenkyū-buntansha) 芝 清隆  財団法人癌研究會, 癌研究所・細胞生物部, 主任研究員 (40196415)
柴田 浩行  財団法人癌研究會, 癌研究所・細胞生物部, 研究員 (50260071)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥4,900,000 (Direct Cost: ¥4,900,000)
Keywordsgene targeting / Cre recombinase / loxP sequences / recombinant adenovirus / transgenic mouse / keratin 14 / Purkinje cell specific protein 2 / olfactory marker protein / Cre遺伝子 / ジーンターゲティング / ケラチン14遺伝子 / PO蛋白遺伝子 / アデノウイルスベクター / コンディショナル・ノックアウト / L7遺伝子 / LoxP配列 / L7プロモーター / コンディショナルジーンターゲティング / PCP2遺伝子
Research Abstract

An establishment of a gene targeting technology allows us to introduce mutations in any target genes on mouse chromosomal DNA.However, since many genes function in developmental stages of mice, mutant mice often suffer embryonic lethality and, therefore, it is difficult to analyze functions of these genes in adult tissues. Conditional gene targeting was invented to circumvent this problem. In this study, we established novel technologies to achieve tissue or cell-lineage specific gene targeting in mice. Our system is based on DNA recombination mediated by Cre recombinase and its recognition sequences, loxP.A pair of loxP sites was introduced into target genes on mouse chromosomal DNA using conventional gene targeting technology. A transgenic allele, which may confer LacZ expression upon Gre-loxP mediated recombination, was also used to validate established technology in this study. To express Cre gene specifically in particular tissues or cells in mice, we developed two novel systems. First, we constructed a recombinant adenovirus carrying Ore gene driven by strong and ubiquitous promoter. By the infection of this virus, we could specifically introduce mutations in target genes in various organs of mice, such as liver, lung, skin, pancreas and intestinal tract. Although the efficiency of gene inactivation is not so high (0.1-3%), we could precisely regulate an onset of gene inactivation using this method. As an altemative way, we also established several lines of transgenic mice, each of which may express Ore recombinase in specific lineage of mice. In this system, promoters of keratin 14 (K14), Purkinje cell specific protein 2 (PCP2), and olfactory marker protein (OMP) genes were successfully used to express Ore gene in epidermal basal cells, cerebellar Purkinje cells and olfactory nerve cells, respectively.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (99 results)

All Other

All Publications (99 results)

  • [Publications] S.Kamada,T.Noda et al.: "bcl-2 Deficiency in mice leads to pleiotropic abnormalities: Accelerated lymphoid cell death in thymus and spleen,polycystic kidney,hair hypopigmentation,and distorted small intestine." Cancer Res.55. 354-359 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Matsuhisa,T.Noda et al.: "Inositol monophoshatase activity from the Escherichia coli suhB gene product." J.Bacteriol.177. 200-205 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Uehara,T.Noda et al.: "Placental defect and embryonic lethality in mice lacking hepatocyte growth factor / scatter factor." Nature. 373. 702-705 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takekura,T.Noda et al.: "Abnormal junctions between surface membrane and sarcoplasmic reticulum in skeltal muscle with a mutation targeted to the ryanodine receptor." Proc.Natl.Acad.Sci.USA. 92. 3381-3385 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takeshima,T.Noda et al.: "Ca^<2+>-induced Ca^<2+>release in myocytes from dyspedic mice lacking the type-1 ryanodine receptor." EMBO J.14(13). 2999-3006 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Miyashiro,T.Noda et al.: "Subcellular localization of the APC protein: Immunoelectron microscopic study of the association of the APC protein with catenin." Oncogene. 11. 89-96 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiba,T.Noda et al.: "Human alanyl-tRNA synthetase: conservation in evolution of catalytic core and microhelix recognition." Biochemistry. 34. 10340-10349 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Sakaguchi,T.Noda et al.: "Accumulation of proteinase K-resistant Prion Protein(PrP) is restricted by the expression level of normal PrP in mice inoculated with a mouse-adapted strain of Creutzfeldt-Jakob disease agent." J.Virol.69. 7586-7592 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Takei,T.Noda et al.: "Synapsin I deficiency results in the structural change in the presynaptic terminals in the murine nervous system." J.Cell Biol.131. 1789-1800 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Nakai,T.Noda et al.: "The POU-domain transcription factor Brn-2 is required for the determination of specific neuronal lineages in the hypothalamus of the mouse." Genes.Dev.9. 3109-3121 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Muto, T.Noda et al.: "Growth retardation in human cervical dysplasia-derived cell lines by β-carotene through down-regulation of epidermal growth factor receptor." Am.J.Clin.Nutr.62. 1535S-1540S (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Matsumoto,T.Noda et al.: "Ataxia and epileptic seizure in mice lacking type 1 inositol 1,4,5-trisphophate receptor." Nature. 379. 168-171 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Sakaguchi,T.Noda et al.: "Loss of cerebellar Purkinje cells in aged mice homozygous for a disrupted PrP gene." Nature. 380. 528-531 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takeshima,T.Noda et al.: "Generation and characterization of mutant mice lacking ryanodine receptor type 3." J.Biol.Chem.271. 19649-19652 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Nagata,T.Noda et al.: "G protein-coupled cholecystokinin-B/gastrin receptors are responsible for physiological cell growth of the stomach mucosa in vivo." Proc.Natl.Acad.Sci.USA. 93. 11825-11830 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Nishi,T.Noda et al.: "Unrestrained nociceptive response and disregulation of hearing ability in mice lacking noniceptin/orphanin FQ receptor." EMBO J.16. 1858-1864 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Suzuki,T.Noda et al.: "A role for macrophage scavenger receptors in atherosclerosis and sesceptibility to infection." Nature. 386. 292-296 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiba,T.Noda et al.: "Creation of libraries with long open reading frames by polymerization of a microgene." Proc.Natl.Acad.Sci.USA. 94. 3805-3810 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Niki,T.Noda et al.: "Hematopoiesis in the fetal liver is impaired by targeted mutagenesis of a gene encoding a non-DNA binding subunit of the transcription factor,PEBP2/CBP." Proc.Natl.Acad.Sci.USA. 94. 5697-5702 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Terunuma,T.Noda et al.: "A novel genetic system to isolate a domonant negative effecter on DNA-binding activity of Oct-2." Nucl.Acid Res.25. 1984-1990 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Wakabayashi,T.Noda et al.: "Genetic and physical delineation of the region of the mouse deafness mouse deafness mutation shaker-2." Biochem.Biophis.Res.Comm.232. 107-110 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Takei,T.Noda et al.: "Delayed development of nervous system in mice homozygous for disrupted microtubule-associated protein 1B(MAp1B)gene." J.Cell Biol.137. 1615-1626 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiba,T.Noda et al.: "Human lysyl-rRNA syntheatase accepts N73 variants and rescues E. coli double-defective mutan." J.Biol.Chem.272. 22809-22816 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Shibata,T.Noda et al.: "Rapid colorectal adenoma formation initiated by conditional targeting of the APC gene." Science. 278. 120-123 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.Ikebe,T.Noda et al.: "Mouse LIM-kinase 2 gene: cDNA cloning,genomic organization and tissue-specific expression of two alternatively initiated transcripts." Genomics. 46. 504-508 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 伊藤正紀、野田哲生 他: "家族性大腸腺腫症(FAP)モデルマウス" 蛋白質 核酸 酵素. 40. 2668-2673 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 柴田浩行、野田哲生 他: "家族性大腸腺腫症モデルマウス" 血液・腫瘍科. 31(6). 449-456 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 柴田浩行、野田哲生: "ジーンターゲティングの新しい戦略-コンディショナルジーンターゲティング法-" 実験医学. 13(1). 73-76 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 野田哲生: "APC遺伝子-その機能と発がんへの関与-" 細胞工学. 14(5). 531-539 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 中川敏幸、野田哲生: "genetargetingによる哺乳類神経発生の研究" 細胞工学. 14(2). 160-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 川野 仁、野田哲生 他: "視床下部神経分泌ニューロンの発生過程とPOU転写調節因子" 神経研究の進歩. 39(5). 785-800 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 伊藤正紀、野田哲生 他: "APC遺伝子と家族性大腸腺腫症疾患モデルマウス" 蛋白質 核酸 酵素. 40(14). 2035-2044 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 野田哲生: "APC遺伝子と家族性大腸腺腫症モデルマウス,消化管癌における最近の話題-胃癌と大腸癌-" (日本医学会,第104回日本医学会シンポジウム記録集). 4-5 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岡田 斉、野田哲生: "ジーンターゲティングの実際" 実験医学. 14(20)増刊. 2710-2714 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 野田哲生: "Genetargetingにおける現在の問題点と今後の展望" 最新医学. 51(6). 1079-1085 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 野田哲生: "発癌研究とジーンターゲティング" 実験医学. 14(20)増刊. 2865-2871 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 柴田浩行、野田哲生: "コンディショナル・ジーンターゲティング-in vivoにおける新しい遺伝子機能解析法-" BIO Clinica. 11(5). 376-380 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 伊藤正紀、野田哲生 他: "がん抑制遺伝子と発がんモデル動物" 最新医学. 51(6). 1153-1160 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 仁木 賢、野田哲生 他: "PEB2/CBF転写因子のターゲティング" 血液 腫瘍科. 34(4). 298-302 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 伊藤正紀、野田哲生: "遺伝子治療のための疾患モデル-FAPモデルマウスとHNPCCモデルマウス-" 肝胆膵. 34(4). 489-494 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 野田哲生: "マウス遺伝学とコンディショナル・ジーンターゲティング" 遺伝子医学. 1(1). 125-126 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 中井茂康、野田哲生: "神経特異的POU転写調節因子群(Brn)の機能解析" 神経研究の進歩. 41. 715-726 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 八尾良二、野田哲生: "ノックアウトマウスを用いたAPCの機能解析" 実験医学. 15(16)増刊. 107-111 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 伊藤正紀、野田哲生: "大腸癌研究の進展-APC遺伝子と大腸癌-" 実験医学. 15(8). 879-884 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 長谷川純崇、野田哲生: "新しいジーンターゲッティングの手法(Cre-loxP)" 内分泌・糖尿病科. 5(4). 396-403 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 柴田浩行、野田哲生 他: "コンディショナル・ジーンターゲティング法による遺伝子機能解析" 癌と化学療法. 24(4). 460-165 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 柴田浩行、野田哲生 他: "疾患モデルマウスによる発癌機構解明の試み" 組織培養工学. 23. 32 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 児玉龍彦、野田哲生: "発生工学による疾病の成因と病態の解析" 現代医療. 29(12). 158-176 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Kamada, T.Noda et al.: "bcl-2 Deficiency in mice leads to pleiotropic abnormalities : Accelaerated lymphoid cell death in thymus and spleen, polycystic kidney, hair hypopigmentation, and distorted small intestine." Cancer Res.55. 354-359 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Matsuhisa, T.Noda et al.: "Inositol monophoshatase activity from the Escherichia colisuhB gene product." J.Bacteriol.177. 200-205 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Uehara, T.Noda et al.: "Placental defect and embryonic lethality in mice lacking hepatocyte growth factor / scatter factor." Nature. 373. 702-705 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takekura, T.Noda et al.: "Abnormal junctions between surface membrane and sarcoplasmic reticulum in skeltal muscle with a mutation targeted to the ryanodine receptor." Proc.Natl.Acad.Sci.USA. 92. 3381-3385 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takeshima, T.Noda et al.: "Ca^<2+>-induced Ca^<2+>release in myocytes from dyspedic mice lacking the type-1 ryanodine receptor." EMBO J.14 (13). 2999-3006 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Miyashiro, T.Noda et al.: "Subcellular localization of the APC protein : Immunoelectron microscopic study of the association of the APC protein with catenin." Oncogene. 11. 89-96 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiba, T.Noda et al.: "Human alanyl-tRNA synthetase : conservation in evolution of catalytic core and microhelix recognition." Biochemistry. 34. 10340-10349 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Sakaguchi, T.Noda et al.: "Accumulation of proteinase K-resistant Prion Protein (PrP) is restricted by the expression level of normal PrP in mice inoculated with a mouse-adapted strain of Creutzfeldt-Jakob disease agent." J.Virol.69. 7586-7592 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Takei, T.Noda et al.: "Synapsin I deficiency results in the structural change in the presynaptic terminals in the murine nervous system." J.Cell Biol.131. 1789-1800 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Nakai, T.Noda et al.: "The POU-domain transcription factor Brn-2 is required for the determination of specific neuronal lineages in the hypothalamus of the mouse." Genes.Dev.9. 3109-3121 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Muto, T.Noda et al.: "Growth retardation in human cervical dysplasia-derived cell lines by beta-carotene through down-regulation of epidermal growth factor receptor." Am.J.Clin.Nutr.62. 1535S-1540S (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Matsumoto, T.Noda et al.: "Ataxia and epileptic seizure in mice lacking type 1 inositol 1,4,5-trisphophate receptor." Nature. 379. 168-171 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Sakaguchi, T.Noda et al.: "Loss of cerebellar Purkinje cells in aged mice homozygous for a disrupted PrP gene." Nature. 380. 528-531 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takeshima, T.Noda et al.: "Generation and characterization of mutant mice lacking ryanodine receptor type 3." J.Biol.Chem.271. 19649-19652 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A,Nagata, T.Noda et al.: "G protein-coupled cholecystokinin-B/gastrin receptors are responsible for physiological cell growth of the stomach mucosa in vivo." Proc.Natl.Acad.Sci.USA. 93. 11825-11830 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Nishi, T.Noda et al.: "Unrestrained nociceptive response and disregulation of hearing ability in mice lacking noniceptin/orphanin FQ receptor." EMBO J.16. 1858-1864 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Suzuki, T.Noda et al.: "A role for macrophage scavenger receptors in atheroxclerosis and susceptibility to infection." Nature. 386. 292-296 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiba, T.Noda et al.: "Creation of libraries with long open reading frames by polymerization of a microgene." Proc.Natl.Acad.Sci.USA. 94. 3805-3810 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Niki, T.Noda et al.: "Hematopoiesis in the fetal liver is impaired by targeted mutagenesis of a gene encoding a non-DNA binding subunit of the transcription factor, PEBP2/CBP." Proc.Natl.Acad.Sci.USA. 94. 5697-5702 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Terunuma, T.Noda et al.: "A novel genetic system to isolate a domonant negative effecter on DNA-binding activity of Oct-2." Nucl.Acid.Res.25. 1984-1990 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Wakabayashi, T.Noda et al.: "Genetic and physical delineation of the region of the mouse deafness mouse deafness mutation shaker-2." Biohem.Biophis.Res.Comm.232. 107-110 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Takei, T.Noda et al.: "Delayed development of nervous system in mice homozygous for disrupted microtubule-associated protein 1B (MAp1B) gene." J.Cell.Biol.137. 1615-1626 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiba, T.Noda et al.: "Human lysyl-tRNA syntheatase accepts N73 variants and rescues E.coli double-defective mutan." J.Biol.Chem.272. 22809-22816 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Shibata, T.Noda et al.: "Rapid colorectal adenoma formation initiated by conditional targeting of the APC gene." Science. 278. 120-123 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.Ikebe, T.Noda et al.: "Mouse LIM-kinase 2 gene : cDNA cloning, genomic organization and tissue-specific expression of two alternatively initiated transcripts." Genomics. 46. 504-508 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Nishi, T.Noda et al.: "Unrestrained nociceptive response and disregulation of hearing ability in mice lacking noniceptin/orphanin FQ receptor." EMBO J.16. 1854-1864 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Suzuki, T.Noda et al.: "A role for macrophage scavenger receptors in atherosclerosis and susceptivility to infection." Nature. 386. 292-296 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Shiba, T.Noda et al.: "Creation of libraries with long open reading frames by polymerization of a microgene." Proc.Natl.Acad.Sci.USA. 94. 3805-3810 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Niki, T.Noda et al.: "Hematopoiesis in the fetal liver is impaired by targeted mutagenesis of a gene encoding a non-DNA binding submit of the transcription factor,PEBP2/CBP." Proc.Natl.Acad.Sci USA. 94. 5697-5702 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Terunuma, T.Noda et al.: "A novel genetic system to isolate a domonant negative effecter on DNA-binding activity of Oct-2." Nucl.Acid.Res.,. 25. 1984-1990 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.wakabayashi, T.Noda et al.: "Genetic and physical delineation of the region of the mouse deafness mutation shaker-2." Biochem.Biophis.Res.Comm.232. 107-110 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Takei, T.Noda et al.: "Delayed development of nervous system inmice homozygous for disrupted microtubuleassociated protein 1B (MAP1B) gene." J.Cell Biol. 137. 1615-1626 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Shiba, T.Noda et al.: "Human lysyl-tRNA synthetase accepts N73 variants and rescues E.coli double-defective mutant." J.Biol.Chem.272. 22809-22816 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Shibata, T.Noda et al.: "Rapid colorectal adenoma formation initiated by conditional targeting of Apc gene." Science. 278. 120-123 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.Aruga, T.Noda et al.: "Mouse Zic1 is involved in cerebellar development." J.Neurosci.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.Toshima, T.Noda et al.: "Structural organization and chromosomal localization of the mouse Tesk1 (testis-specific protein kinase 1) gene." Gene. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] C.Ikebe, T.Noda et al.: "Mouse LIM-kinase 2 gene : cDNA cloning,genomic organization and tissue-specific expression of two alternatively initiated transcripts." Genomics. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto,M.,Noda,T.,et al.: "Ataxia and epileptic seizure in mice lacking type 1 inositol 1,4,5-trisphophate receptor." Nature. 379. 168-171 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakaguchi,S.Noda,T.,et al.: "Loss of cerebellar Purkinje cells in aged mice homozygous for a disrupted PrP gene." Nature. 380. 528-531 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takeshima,H.,Noda,T.,et al.: "Generation and characterization of mutant mice lacking ryanodine receptor type 3." J.Biol.Chem.271. 19649-19652 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagata,A.,Noda,T.,et al.: "G protein-coupled cholecystokinin-B/gastrin receptors are responsible for physiological cell growth of the stomach mucosa in vivo." Proc.Natl.Acad.Sci.USA. 93. 11825-11830 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nishi,M.,Noda,T.,et al.: "Unrestrained nociceptive response and disregulation of hearing ability in mice lacking noniceptin/orphaninFQ receptor." EMBOJ.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki,H.,Noda,T.,et al.: "A role for macrophage scavenger receptors in atherosclerosis and susceptibility to infection." Nature. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shiba,K.,Noda,T.,et al.: "Creation of libraries with long open reading frames by polymerization of a microgene." Proc.Natl.Acad.Sci.USA. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Niki,M.,Noda,T.,et al.: "Hematopoiesis in the fetal liver is impaired by targeted mutagenesis of a gene encoding a non-DNA binding subunit of the transcripion factor,PEBP2/CBP." Proc.Natl.Acad.Sci.USA. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Uehara: "Placental defect and embryonic lethality in mice lacking hepatocyte growth factor/scatter factor" Nature. 373. 702-705 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Shiba: "Human alanyl-tRNA synthetase:conservation in evolution of catalytic core and microhelix recognition." Biochemistry. 34. 10340-10349 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Nakai: "The POU-domain transcription factor Brn-2 is required for the determination of specific neuronal lineages in the hypothalamus of the mouse." Genes.Dev.9. 3109-3121 (1995)

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  • [Publications] Y.Muto: "Growth retardation in human cervical dysplasia-derived cell lines by β-carotene through down-regulation of epidermal growth factor receptor." Am.J.Clin.Nutr.(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Matsumoto: "Ataxia and epileptic seizure in mice lacking type 1 inositol 1,4,5-trisphophate receptor." Nature. 379. 168-171 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Sakaguchi: "Loss of cerebellar Purkinje cells in aged mice homozygous for disrupted PrP gene." Nature. (in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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