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Dynamics of postsynaptic calcium involved in plasticity of visual cortex

Research Project

Project/Area Number 07458220
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 神経・脳内生理学
Research InstitutionOsaka University

Principal Investigator

TSUMOTO Tadaharu  Osaka University Medical School, Professor, 医学部, 教授 (50028619)

Co-Investigator(Kenkyū-buntansha) HATA Yoshio  Osaka University Medical School, Research Associate, 医学部, 助手 (40212146)
畠 義郎  大阪大学, 医学部, 助手 (20089882)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsSynaptic plasticity / Visual cortex / Long-term potentiation / Long-term depression / Development / Rat / Calcium / Memory / 脳 / 神経回路 / カルシウムイメージング / ニューロン
Research Abstract

In the developing visual cortex, repetitive stimulation of afferent pathways was reported to induce long-term potentiation (LTP) or long-term depression (LTD) of synaptic transmission in layr II/III of the cortex. It was not clearly known, however, what factors determine the direction of long-term changes in synaptic efficacy, i.e., LT or LTD.The present results were designed to address this question.
Thin slices of the visual cortex were prepared from young rats (postnatal days 20-26). Under visual control, neurons in layr II/III were loaded with a fluorescent Ca^<2+> indicator, rhod 2, through glass micropipettes for patch-clamp recording. Changes in fluorescence intensity were recorded simultaneously with excitaory postsynaptic potentials (EPSPs) evoked by test stimulation of layr IV.
Tetanic stimulation of layr IV which induced LTP of EPSPs led to a massive increase in fluorescence intensity at dendritic hot spots. On the other hand, tetanic stimulation which induced a moderate rise of fluorescence led to LTD of EPSPs. In sum, it is suggested that a rise of postsynaptic Ca^<2+> during synaptic inputs beyond a certain threshold induces LTP while a weak or moderate rise below the threshold induces LTD.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Yasuda, H.and Tsumoto, T.: "Long-term depression in visual cortex is associated with a lower rise of postsynaptic Ca^<2+> than LTP" Neuroscience Research. 24. 265-274 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akaneya, Y.et al.: "Long-term depression blocked by brain derived neurotrophic factor in rat visual cortex" Journal of Neurophysiology. 76. 4198-4201 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura, F.et al.: "Presynaptically silent synapses:Spontaneously active terminals without stimulus-evoked release demonstrated in cortical autapses" Journal of Neurophysiology. 77. 2805-2815 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Torii, N.et al.: "Quantal analysis of presynaptic involvement in expression of neocortical short-and long-term depression" Neuroscience. 79. 317-321 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akaneya, Y.et al.: "Brain-derived neurotrophic factor enhances long-term potentiation in rat visual cortex" Journal of Neuroscience. 17. 6707-6716 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuda, H.et al.: "Local contribution of NMDA receptors to synaptic input-induced rise of calcium in apical dendrites of layer II/III neurones in rat visual cortex" Neuroscience. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuda, H.and Tsumoto, T.: "Long-term depression in visual cortex is associated with a lower rise of postsynaptic Ca^<2+> than LTP." Neuroscience Research.24. 265-274 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akaneya, Y.et al.: "Long-term depression blocked by brain derived neurotrophic factor in rat visual cortex." Journal of Neurophysiology. 76. 4198-4201 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura, F.et al.: "Presynaptically silent synapses : Spontaneously active terminals without stimulus-evoked release demonstrated in cortical autapses." Journal of Neurophysiology. 77. 2805-2815 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Torii, N.et al.: "Quantal analysis of presynaptic involvement in expression of neocortical short- and long-term depression." Neuroscience. 79. 317-321 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akaneya, Y.et al.: "Brain-derived neurotrophic factor enhances long-term potentiation in rat visual cortex." Journal of Neuroscience. 17. 6707-6716 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuda, H.et al.: "Local contribution of NMDA receptors to synaptic input-induced rise of calcium in apical dendrites of layr II/III neurones in rat visual cortex" Neuroscience. (in press). 1998

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura,F.,Otsu,Y.and Tsumoto,T.: "Presynaptically silent synapses : Spontaneously active terminals without stimulus-evoked release demonstrated in cortical autapses." J.Neurophysiol.77(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akaneya,Y.,Tsumoto,T.,and Hatanaka,H.: "Brain-derived neurotrophic factor blocks long-term depression in rat visual cortex." J.Neurophysiol.76. 4198-4201 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsumoto,T.and Yasuda,H.: "A switching role of postsynaptic calcium in the induction of long-term potentiation or long-term depression in visual cortex." Seminars in Neurosci.8. 311-319 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sato,H.,Katsuyama,N.,Tamura,H.,Hata,Y.and Tsumoto,T.: "Mechanisms underlying orientation selectivity of neurons in the primary visual cortex of the macaque." J.Physiol.Lond.494. 757-771 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tamura,H.,Sato,H.,Katsuyama,N.,Hata,Y.,and Tsumoto,T.: "Less segregated processing of visual information in V2 than in V1 of the monkey visual cortex." Europ.J.Neurosci.8. 300-309 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuda,H.and Tsumoto,T.: "Long-term depression in visual cortex is associated with a lower rise of postsynaptic Ca2+ than LTP." Neurosci.Res.24. 265-274 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Otsu,Y.,Kimura,F.and Tsumoto,T.: "Hebbian induction of LTP in visual cortex : A perforated patch clamp study in cultured neurons." J.Neurophysiol.74. 2437-2444 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Torii,N.,Kamishita,Y.,Otsu,Y.and Tsumoto,T.: "An inhibitor for calcineurin,FK506 blocks induction of long-term depression in rat visual cortex." Neurosci.Lett.185. 1-4 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kamishita,T.,Haruta,H.,Torii,N.,Tsumoto,T.and Hicks,T.P.: "Metabotropic glutamate receptors and visual cortical synaptic plasticity." Can.J.Physiol.Pharmacol.73. 1312-1322 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato,H.,Tamura,H.,Katksuyama,N.,Hata,Y.and Tsumoto,T.: "Mechanisms underlying direction selectivity of neurons in the primary visual cortex of the macaque." J.Neurophysiol.74. 1382-1394 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tamura,H.,Sato,H.,Katsuyama,N.,Hata,Y.,and Tsumoto,T.: "Less segregated processing of visual information in V2 than V1 of the monkey visual cortex." Europ.J.Neurosci.8. 300-309 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasuda,H.and Tsumoto,T.: "Long-term depression in visual cortex is associated with a lower rise of postsynaptic Ca2+ than LTP." Neurosci.Res.(in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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