| Project/Area Number |
07458241
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Research Institution of National Cardiovascular Center |
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Principal Investigator |
MATSUDA Takehisa National Cardiovascular Center-Department of Bioengineering, Head, 生体工学部, 部長 (60142189)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHIZAKI Yoshihide The Institute of Physical and Chemical Research, Researcher, 研究センタージーンバンク室・真核生物研究室, 主任研究員 (70192705)
TANAHASHI Masami National Cardiovascular Center-Department of Bioengineering, Researcher, 生体工学部, 流動研究員
TAKAICHI Shigeko National Cardiovascular Center-Department of Structural Analysis, Senior Staff, 循環器形態部, 室長 (00093930)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1996: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥5,300,000 (Direct Cost: ¥5,300,000)
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| Keywords | Mechanical Stress / Vascular Cells / Extracellular Matrix |
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| Research Abstract |
This study was conducted to study the responses of cells exposed to mechanical stress fild, which include orientation and elongation of adhered cells on 2-dimensional substrate in vitro and morphogenesis and tissue formation of vascular wall in vivo under continuously loaded mechanical stress fields.
Upon continuous cyclic distension of adhered cells, cells oriented parallel to the direction of distension with loading time of mechanical stress. On the other hand, hybrid vascular grafts prepared using vascular cell types (endothelial cell ; EC,smooth muscle cell ; SMC and fibroblast ; FC) underwent to remodel themselves upon implantation. ECs highly elongated to the direction of flow, whereas SMCs are circumferentially oriented. Extracellular matrix (ECM) polymers secreted by cells underwent self-assembling and organized to form fiber formation with time. These ECM polymers include collagen and elastin. The former constructed a network of fiber highly oriented circumfererfially, and th later formed a layred honey-comb structure. Thus, sellular and macromolecular orientation proceeded in time to restore vital structure and function of neoarterial vessels.
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