| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Tasunori Assoc, Prof., Fukuyama University, Faculty of Engineering, Fukuyama, Japan, 工学部・生物工学科, 助教授 (60191243)
SHIROISHI Toshihiko Assoc, Prof., National Institute of Gentics Mishima, Japan, 生命科学研究科・遺伝学専攻, 助教授 (90171058)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Research Abstract |
From east Asia including mainland China, north of Yangtze River, Korea and Japan, to eastern Europe, a house mouse subspecies, MUS musculus musculus, and some other genetically close ones inhabit. We designated them musculus subspecies group. In southern China, south of Yangtze River, and southeast Asia such as the Philippines, Taiwan and Indonesia, castaneus subspecies group of mice inhabit. In western Europe, domesticus subspecies group is predominant. Genetic divergence among those three subspecies groups has been estimated roughly one million years based on genetic variations in mitochondrial DNAs, ribosomal DNAs, biochemical genes, chromosome C-bands, immunological genes etc. The number of genetic loci so faranalyzed, however, was not enough to obtain highly plausible estimation of genetic divergence among those subspecies groups. The present study attempted to get the more reliable estimation of it by using the larger number of loci. We employed 60 kinds of MIT PCR primers to det
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ect the length polymorphism of microsatellite DNAs. Eight kinds of inbred strains established in National Institute of Genetics in Mishima from various wild mice covering the three major subspecies groups were used in the present analyzes. MAL strain originated from Malysia, CAST from Thailand and HMI from Taiwan in castaneus subspcies group, MSM from Japan, SWN and KJR from Korea, BLG2 form Bulgaria and NJL from Denmark in musculus group, and BFM from France in domesticus group were analyzed. Dendrogram constructed from the length polymorphism of microsatellite DNAs by NJ method well agreed with the previous one mentioned above. Only discrepancy in this analysis with the previous one was BLG2 which was estimated as domesticus group, not musculus one, Since the number of lici compared in the present assay were considerably large, BLG2 should be included in domesticus subspecies group, though the genetic introgression of musculus group to some extent could not be excluded. Estimation of genetic distance between two populations based on the relatively larger number of polymorphic microsatellite DNAs seems to be considerably reliable. Less
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