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Respiratory chain of Gram-positive bacteria for better energy yield

Research Project

Project/Area Number 07459018
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 広領域
Research InstitutionKyushu Institute of Technology

Principal Investigator

SONE Nobuhito  Kyushu I.of Techn., 情報工学部, 教授 (20049034)

Co-Investigator(Kenkyū-buntansha) NOGUCHI Shunsuke  Kyushu I.of Techn., 情報工学部, 助手 (30222194)
SAKAMOTO Junshi  Kyushu I.of Techn., 情報工学部, 助教授 (80175364)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordsrespiratory chain / H^+ / O ratio / growth yield / cytopchrome oxidase / cytochrome reductase / Bacillus stearothermophilus / Coryne-form bacteria / Helicobacter pylori / 呼吸鎖 / プロトン輸送 / シトクロムオキシダーゼ / キノールオキシダーゼ / グラム陽性細菌 / Bacillus / 好熱性細菌 / 枯草菌 / Bacillus stearother mophilus / Carynebacterium / Baciluus / Brevibacterium
Research Abstract

The following results were obtained on the energy yield of usuful bacteria and its inovation.
1. Respiratory chain of thermophilic bacilli was analyzed to be composed of NADH dehydrogenase- MK-7- b6c1 complex- caa3 oxidase- O_2. We also found cytochrome bd-type quinol oxidase and cytochrome b(o/a) 3-type cytochrome c-551 oxidase are working.
2. In mesophilic bacilli cytochrome aa3-type quinol oxidase was the main terminal oxidase, and thus this is the reason for low H^+/O ratio around 4 of these bacteria.
3. To compare these heme-Cu oxidase family, Helicobacter pylori cytochrome cb-type cytohrome oxidase and its substrate cytochrome c-553 were investigated.
4. Using Bacillus stearothermophilus and its mutant which lacks caa3-type cytochrome c oxidase and contains bd-type quinol oxidase as the main terminal oxidase, direct corelationship between H^+/O ratio of the cell as the combination of H^+-pumping respiratory complexes and the growth yield of the bacterium.
5. The respiratory chain of Coryne-form bacteria, especially used in amino acid fermentation was examined, and two terminal oxidase, bd-type quinol oxidase and aa3-type cytochrome oxidase were purified. We also succeeded in cloning their structure genes.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Kusano,Kuge.Sakamoto Noguchi.Sone: "Nucleotide amino acid sequences for cytochrome caa3-type oxidase of Bacillus stearothermophilus K1041 and non-Michaelis-typc kinetics with cytochrome c" Biochim. Biophys. Acta. 1273. 129-138 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sakamoto,Matsumoto Ohbuchi,Sone: "Cytochrome bd-type quinol oxidase in a mutant of Bacillus stearothermophilus deficient in caa3-type cytochrome c oxidase" FEMS Microbiol. Lett.143. 151-158 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sone.Tsuchiya.Inoue.Noguchi.Sone: "Bacillus stearothermophilus qcr operon encoding Rieske FeS protein,cytochrome b6 and a novel-type cytochrome cl of quinol-cytochrome c reductase." J.Biol. Chem.271. 12457-12462 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yaginuma,Tsukita Sakamoto,Sone: "Characterization of two terminal oxidase in Bacillus brevis and efficiency of energy conservation of the respiratory chain." J.Biochem.122. 969-976 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sakamoto,Handa Sone: "A novel cytochrome b(o/a)3-type oxidase from Bacillus stearothermophilus catalyzes cytochrome c-551 oxidation." J.Biochem.122. 969-976 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nikaido,Noguchi:Sakamoto. Sone: "The cbaAB genes for bo3-type cytochrome coxidase in Bacillus stearothermo philus." Biochim. Biophys. Acta. 1397. 262-267 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 石村 光、曽根 他: ""Oxygen Homeostasis and its dynamics"" Springer Verlag, Tokyo, (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tashiro, H., Sone, N.: "Preparation and characterization of the hydrophilic CuA-cytochromec domain of subunit II of cytochrome c oxidase from thermophilic Bacillus PS3." J.Biochemistry. 117. 521-526 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sone, N.Sawa, G., Sone, T., Noguchi, S.: "Thermophilic bacillihave split cytochrome b genes for cytochrome b6 and subunit IV." J.Biol.Chem.270. 10612-10617 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kusano, T., Kuge, S., Sakamoto, J., Noguchi, S.Sone, N.: "Nucleotide amino acid sequences for cytochrome caa3-type oxidase of Bacillus stearothermophilus K1041 and non-Michaelis-type kinetics with cytochrome c." Biochim.Biophys.Acta. 1273. 129-138 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuge, S., Noguchi, S., Sakamoto.J.and Sone, N.: "Identification of peptide fragments chemically cross-linked in cytochrome c oxidase from thermophilic Bacillus PS3." Biochem.Mol.Biol.Int.38. 181-188 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirota, S., Svenson, B., Adelroth, P., Sone, N.Nilsson, T., Malmstrom, B.GBrezezinski, P.: "A flash-photolysis study of the reactions of a caa3-type cytochrome c oxidase with dioxygen and carbon monoxide." J.Bioenerg.Biomembr. vol.28. 497-501

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sakamoto, J., Matsumoto, A., Oobuchi, K.and Sone, N.: "Cytochrome bd-type quinol oxidase in a mutant of Bacillus stearothermophilus deficient in caa3-type cytochrome c oxidase" FEMS Microbiol.Lett.143. 151-158 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagata, K., Tsukita, S.Tamura, T.and Sone, N.: "A cb-type cytochrome c oxidase terminates the respiratory chain in Helicobacter pylori." Microbiology. 142. 1757-1763 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sone, N., Tsuchiya, N., Inoue, M.and Noguchi, S.: "Bacillus stearothermophilus qcr operon encoding Rieske FeS protein, cytochrome b6 and a novel-type cytochrome c1 of quinol-cytochrome c reductase" J.Biol.Chem. 271. 12457-12462 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kai, K., Noguchi, S., Sone, N.: "Over-expressionand post-translational modification of thermophilic Bacillus cytochrome c-551 in Bacillus subtilis." J.Ferm.Bioenginer.84. 190-194 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uchida, A., Kusano, T., Mogi, T., Anraku, Y., Sone, N.: "Expression of Escherichia coli bo-type ubiquinol oxidase with a chimeric subunit II having the CuA cytochrome c domain from the thermophilic Bacillus caa3-type cytochrome c oxidase." J.Biochem. 122. 1004-1009 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yaginuma, A., Tsukita, S., Sakamoto, J., Sone, N.: "Characterization of two terminal oxidase in Bacillus brevis and efficiency of energy conservation of the respiratory chain." J.Biochem. 122. 969-976 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sakamoto, J., Handa, Y., Sone, N.: "A novel cytochrome b (o/a) 3-type oxidase from Bacillus stearothermophiluscatalyzes cytochrome c-551 oxidation." J.Biochem. 122. 764-771 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nikaido, K., Noguchi, S., Sakamoto, J., Sone, N.: "The cbaAB genes for bo3-type cytochrome c oxidase in Bacillus stearothermophilus." Biochim.Biophys.Acta. 1397. 262-267 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uchida,A.,Kusano,T.Mogi,T.,Anraku,Y.and Sone,N.: "Expression of Escherichia coli bo-type ubiquinol oxidase with a chimeric subunit II having the CuA-cytochrome c domain from the thermophilic Bacillus caa3-type cytochrome c oxidase" J.Biochem.122・5. 1004-1009 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yaginuma,A.,Tsukita,S.,Sakamoto,J.and Sone,N.: "Characterization of two terminal oxidase in Bacillus brevis and efficiency of energy conservation of the respiratory chain." J.Biochem.122・5. 969-976 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kai,K.,Noguchi,S.and Sone,N: "Over-expression and post-translational modification of thermophilic Bacillus cytochrome c-551 in Bacillus subtilis.J.Ferm.Bioenginer.84," J.Ferm.Bioenginer.84・3. 190-194 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sakamoto,J.,Handa,Y.and Sone,N.: "A novel cytochrome b(o/a)3-type oxidase-type from Bacillus stearothermophilus catalyzes cytochrome c-551 oxidation." J.Biochem.122・4. 764-771 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishimura,Y.,Sone,N et,al: "Oxygen homeostasis and its dynamics" Springer,Tokyo, 620(112-119) (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sakamoto,J.,Matsumoto,A.Oobuchi,K.and Sone,N.: "Cytochrome bd-type quinol oxdase in a mutant of Bacilus stearothermophilus deficient in caa3-type cytochrome c oxidase" FEMS Microbiol.Lett.,143,151-158. 143. 151-158 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sone,N.,Tsuchiya,N.,Inoue,M.and Noguchi,S.: "Bacillus stearothermophilus qcroperon encoding Rieske Fe Sprotein,cytochrome b6 and a novel-type cytochrome c1 of quinol-cytochrome c reductase." J.Biol.Chem.271,12457-12462. 271. 12457-12462 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagata,K.,Tsukita.S.,Tamura,T.and Sone,N.: "A cb-type cytochrome c oxidase terminates the rrespiratory chain in Helicobacter pylori." Microbiology,142,1757-1763. 142. 1757-1763 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tanaka,T.,Inoue,M.,Sakamoto,J.and Sone,N.: "Intra-and Inter-complex cross-linking of subunits in the quinol oxidase super-complex from thermophilic Bacillus PS3." J.Biochem.119. 482-486 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tashiro,H and Sone,N.: "Preparation and characterization of the hydropholic CuA-cytochrome c domain of subunit II of cytochrome c oxidase from thermophilic Bacillus PS3" (1995) J.Biochem.117. 521-526 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sone,N.,Sawa,G.,Sone,T.and Noguchi,S.: "Thermophilic Bacilli have split cytochrome b genes for cytochrome b6 and subunit IV." J.Biol.Chem.270. 10612-10617 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kusano,T.,Kuge,S.,Sakamoto,J.,Noguchi,S.Sone,N.: "Nucleotide amino acid sequences for cytochrome caa3-type oxidase of Bacillus stearothermophilus K1041 and non-Michaelis-type kinetics with cytochrome c" Biochim.Biophys.Acta. 1273. 129-138 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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