| Project/Area Number |
07459018
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
広領域
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
SONE Nobuhito Kyushu I.of Techn., 情報工学部, 教授 (20049034)
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Shunsuke Kyushu I.of Techn., 情報工学部, 助手 (30222194)
SAKAMOTO Junshi Kyushu I.of Techn., 情報工学部, 助教授 (80175364)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | respiratory chain / H^+ / O ratio / growth yield / cytopchrome oxidase / cytochrome reductase / Bacillus stearothermophilus / Coryne-form bacteria / Helicobacter pylori / 呼吸鎖 / プロトン輸送 / シトクロムオキシダーゼ / キノールオキシダーゼ / グラム陽性細菌 / Bacillus / 好熱性細菌 / 枯草菌 / Bacillus stearother mophilus / Carynebacterium / Baciluus / Brevibacterium |
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| Research Abstract |
The following results were obtained on the energy yield of usuful bacteria and its inovation.
1. Respiratory chain of thermophilic bacilli was analyzed to be composed of NADH dehydrogenase- MK-7- b6c1 complex- caa3 oxidase- O_2. We also found cytochrome bd-type quinol oxidase and cytochrome b(o/a) 3-type cytochrome c-551 oxidase are working.
2. In mesophilic bacilli cytochrome aa3-type quinol oxidase was the main terminal oxidase, and thus this is the reason for low H^+/O ratio around 4 of these bacteria.
3. To compare these heme-Cu oxidase family, Helicobacter pylori cytochrome cb-type cytohrome oxidase and its substrate cytochrome c-553 were investigated.
4. Using Bacillus stearothermophilus and its mutant which lacks caa3-type cytochrome c oxidase and contains bd-type quinol oxidase as the main terminal oxidase, direct corelationship between H^+/O ratio of the cell as the combination of H^+-pumping respiratory complexes and the growth yield of the bacterium.
5. The respiratory chain of Coryne-form bacteria, especially used in amino acid fermentation was examined, and two terminal oxidase, bd-type quinol oxidase and aa3-type cytochrome oxidase were purified. We also succeeded in cloning their structure genes.
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