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The development of increasing the energetic efficiency of failing heart : Application of Emax and PVA

Research Project

Project/Area Number 07508003
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionOkayama University

Principal Investigator

SUGA Hiroyuki  Okayama Univ.Med.Sch., Dept.of Physiol.II,Professor, 医学部, 教授 (90014117)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Juichiro  Okayama Univ.Med.Sch., Dept.of Physiol.II,Assistant Professor, 医学部, 助手 (80294403)
MIKANE Takashi  Okayama Univ.Med.Sch., Dept.of Physiol.II,Assistant Professor, 医学部, 助手 (10294406)
ARAKI Junichi  Okayama Univ.Med.Sch., Dept.of Physiol.II,Assistant Professor, 医学部, 助手 (80271055)
松原 広己  岡山大学, 医学部, 助手 (70252955)
高木 都  岡山大学, 医学部, 助教授 (00033358)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥26,800,000 (Direct Cost: ¥26,800,000)
Fiscal Year 1997: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1996: ¥9,100,000 (Direct Cost: ¥9,100,000)
Fiscal Year 1995: ¥13,700,000 (Direct Cost: ¥13,700,000)
Keywordsfalling heart / postextrasystolic potentiation / excitation-contraction coupling / beat constant / recirculation fraction / total Ca^<2+> handling / Emax / PVA / Emaxの酸素コスト / 心筋スライス / 酸素消費量
Research Abstract

We found that all postextrasystolic contractile potentiation decayd in alternans with the exception of particular cases in excised cross-circulated canine left ventricles even under physiological conditions. Our analysis showed the transient alternans to consist of two comoponens, exponential and sinusoidal decay. So we hypothesized that the normalized pressure (y) of postextrasystolic beats (beat number x) could be expressed by the following equation.
y=a ・ exp [- (x-1) /tau_e] +b ・ exp [- (x-1) /tau_s] Xcos [pi (x-1)] +1
This equation very closely fitted the alternans data with a squared corelation coefficient of 0.9996 on average. Then, we speculated that the exponential component of this equation would be related to the intracellular Ca ^<2+> recirculation fraction (RF) and the sinusoidal component to Ca^<2+> handling by the sarcoplasmic reticulum.
We investigated the effects of intracoronary Ca^<2+> and epinephrine on the intracellular Ca^<2+> RF and total Ca^<2+> handling in the lef … More t ventricle. RF was calculated from tau_s by exp (-1/tau_s). Ca^<2+> slightly increased RF but epinephrine scarcely affected it althoug both drugs enhanced Emax (an index of con) 2-3 times. We also obtained the total amount of Ca^2 handled in one cardiac cycle by combining these RF data with Ca^<2+> handling Vo_2 data.
We observed disproportionately high E-C coupling Vo_2 and decreased RF when intracoronarily infused ryanodine (approximately 40nmol/l blood for 1h) decreased Emax. We considered that the decreased RF indicated wasteful myocardial Ca^<2+> handling for a greater fraction of transsarcolemmal Ca^<2+> handling which was half economical of intracellular Ca^<2+> handling via the sarcoplasmic reticulum.
We conclude that analysis of postextrasystolic potentiation in various situation s enables us to better understand pathophysiology of failing hearts and to evaluate pharmacology of cardiotonic agents from anew stand point. No other methods can evaluate intracellular total Ca^<2+> handling in the whole heart like this method. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (51 results)

All Other

All Publications (51 results)

  • [Publications] Shimizu J, Takaki M, Kohno K, Araki J, Matsubara H, Suga H: "Sinusoidal and exponential decays of postextrasystolic transient alternans in excised blood-perfused canine hearts." Japanese Journal of Physiology. 45. 837-848 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsushita T, Takaki M, Fujii W, Matsubara H, Suga H: "Left ventricular mechanoenergetics under altered coronary perfusion in guinea pig hearts." Japanese Journal of Physiology. 45. 991-1004 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhao LY, Takaki M, Fujii W, Ishioka K, Suga H: "Discrepancy between left ventricular mechanoenergetics and mitochondrial respiratory function in canine acute failing hearts." Pathophysiology. 2. 211-216 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuhara S, Takaki M, Kikuta A, Suga H: "Myocardial Vo_2 of mechanically unloaded contraction of rat ventricular slices measured by a new approach." American Journal of Physiology. 270. H1063-H1070 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamaguchi H, Takaki M, Matsubara H, Yasuhara S, Suga H: "Constancy and variability of contractile efficiency as a function of calcium and crossbridge kinetics : simulation." American Journal of Physiology. 270. H1501-H1508 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito H, Takaki M, Yamaguchi H, Tachibana H, Suga H: "Left ventricular volumetric conductance catheter for rats." American Journal of Physiology. 270. H1509-H1514 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takaki M, Nakahara H, Kawatani Y, Utsumi K, Suga H: "No Suppression of respiratory function of mitochondria isolated from hearts of anesthetized rats with high-dose pentobarbarbital sodium." Japanese Journal of Physiology. 47. 87-92 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tachibana H, Takaki M, Lee S, Ito H, Yamaguchi H.Suga H: "New mechanoenergetic evaluation of left ventricular contractility in in situ rat hearts." American Journal of Physiology. 41. H2671-H2678 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mikane T, Araki J, Kohno K, Nakayama Y, Suzuki S, Shimizu J, Matsubara H, Hirakawa M, Takaki M, Suga H: "Mechanism of constant contractile efficiency under cooling inotropy of myocardium : simulation." American Journal of Physiology. 42. H2891-H2898 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hata Y, Shimizu J, Hosoki S, Matsubara H, Araki J, Ohe T, Takaki M, Takasago T, Tad W Taylor, Suga H: "Ryanodine decreases Internal Ca^<2+> recirculation fraction of the canine heart as sutudied by postextrasystolic transient alternans" Japanese Journal of Physiology. 47. 521-530 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Syuu Y, Araki J, Lee S, Suzuki S, Mizuno J, Mohri S, Mikane T, Shimizu J, Takaki M, Suga H: "Effects of Ca^<2+> and epinephrine on Ca^<2+> recirculation fraction and total Ca^<2+> handling in canine left ventricles." Japanese Journal of Physiology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takaki M, Matsubara H, Araki J, Zhao LY, Ito H, Yasuhara S, Fujii W, Suga H: "Sasayama S(Ed:)New Horizons for Failing Heart syndrome" Springer-Verlag Tokyo, 273 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suga H, Yamaguchi H, Araki J, suzuki S, Shimizu J, Matsubara H, Kawaguchi O, Yaku H, Takaki M: "Akutsu T,Koyanagi H(Eds:)Heart Replacement : Artificial Heart 6" Springer-Verlag Tokyo, 521 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimizu J,Takaki M,Kohno K,Araki J,Matubara H,Suga H: "Sinusoidal and exponential decays of postextrasystolic transient alternas in excised blood-perfused canine heart" Japanese Journal of Physiology. Vol.45. 837-848 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsushita T,Takaki M,Fujii W,Matubara H,Suga H: "Left ventricular mechanoenergetics under altered coronary perfusion in guine a pig hearts" Japanese Journal of Physiology. Vol.45. 991-1004 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhao LY,Takaki M,Fujii W,Ishioka K,Suga H: "Discrepancy between left ventricular mechanoenergetics and mitochondrial respiratory function in canine acute falling heart" Pathophysiology. Vol.2. 211-216 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuhara S,Takaki M,Kikuta A,Suga H: "Myocardial VO2 of mechanically unloaded contraction of rat ventricular slices measured by a new approach" American journal of Physiology. Vol.270. H1063-H1070 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamaguchi H,Takaki M,Matsubara H,Yasuhara S,Suga H: "Constancy and variability of contractile efficiency as a function of calcium and cross-bridge kinetics : simulation" American journal of Physiology. Vol.270. H1501-H1508 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito H,Takaki M,Yamaguchi H,Tachibana H,Suga H: "Left venticular volumetric condactance catheter for rats" American journal of Physiology. Vol.270. H1509-H1514 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takaki M,Nakahara H,Kawatani Y,Utsumi K,Suga H: "No suppression of respiratory function of mitochondria isolated from hearts of anesthetized rats with high-dose pentobarbital sodium" Japanease Journal of Physiology. Vol.47. 87-92 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tachibana H,Takaki M,Lee S,Ito H,Yamaguchi H Suga H: "New mechanoenergetic evaluation of ventricular contractility in situ rat heart" American journal of Physiology. Vol.41. 2671-2678 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mikane T,Araki J,Kohno K,Nakayama Y,Suzuki S,Shimizu J,Matsubara H,Hirakawa M,Takaki M,Suga H: "Mechanism of constant contractile efficiency under cooling inotropy of myocardium : simulation" American journal of Physiology. Vol.4. H2891-H2898 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Syuu Y,Araki J,Lee S,Suzuki S,Mizuno J,Mohri S,Mikane T,Shimizu J,Takaki M,Suga H: "Effects of Ca^<2+> and epinephrine on Ca^<2+> recirculation fraction and total Ca^<2+> handling in canine left ventricle" Japanease Journal of Physiology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hata Y,Shimizu J,Hosogi S,Matsubara H,Araki J,Ohe T,Takaki M,Takasago T,Tad W.Taylor, and Suga H: "Ryanodine decreases internal Ca^<2+> recirculationfraction of the canine heart as studied by postextrasystolic transient alternans" Japanease Journal of Physiology. Vol.47. 521-530 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takaki M,Matsubara H,Araki J,Zhao LY,Ito H,Yasuhara S,Fujii W,Suga H: Sasayama S (Ed : ) New Horizons for Falling Heart Syndrome. Springer-Verlag Tokyo, (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suga H,Yamaguchi H,Araki J,Suzuki S,Shimizu J,Matsubara H,Kawaguchi O,Yaku H,Takaki M: Akutu T,Koyanagi H,(Eds : ) Heart Replacement : Artificial Heart 6. Sprigner-Verlag Tokyo, (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimizu J, Takaki M, Kohno K, Araki J, Matsubara H, Suga H: "Sinusoidal and exponential decays of postextrasystolic transient alternans in excised blood-perfused canine hearts." Japanese Journal of Physiology. 45. 837-848 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsushita T, Takaki M, Fujii W, Matsubara H, Suga H: "Left ventricular mechanoenergetics under altered coronary perfusion in guinea pig hearts." Japanese Journal of Physiology. 45. 991-1004 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Zhao LY, Takaki M, Fujii W, Ishioka K, Suga H: "Discrepancy between left ventricular mechanoenergetics and mitochondrial respiratory function in canine acute failing hearts." Pathophysiology. 2. 211-216 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuhara S, Takaki M, Kikuta A, Suga H: "Myocardial VO_2 of mechanically unloaded contraction of rat ventricular slices measured by a new approach." American Journal of Physiology. 270. H1063-H1070 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamaguchi H, Takaki M, Matsubara H, Yasuhara S, Suga H: "Constancy and variability of contractile efficiency as a function of calcium and cross-bridge kinetics : simulation." American Journal of Physiology. 270. H1501-H1508 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ito H, Takaki M. Yamaguchi H, Tachibana H, Suga H: "Left ventricular volumetric conductance catheter for rats." American Journal of Physiology. 270. H1509-H1514 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takaki M, Nakahara H, Kawatani Y, Utsumi K, Suga H: "No Suppression of respiratory function of mitochondria isolated from hearts of anesthetized rats with high-dose pentobarbarbital sodium." Japanese Journal of Physiology. 47. 87-92 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tachibana H, Takaki M, Lee S, Ito H, Yamaguchi S, Suga H: "New mechanoenergetic evaluation of left ventricular contractility in in situ rat hearts." American Journal of Physiology. 41. 2671-2678 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mikane T, Araki J, Kohno K, Nakayama Y, Suzuki S, Shimizu J, Matsubara H, Hirakawa M, Takaki M, Suga H: "Mechanism of constant contractile efficiency under cooling inotropy of myocardium : simulation." American Journal of Physiology. 42. H2891-H2898 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Syuu Y, Araki J, Lee S, Suzuki S, Mizuno J, Mohri S, Mikane T, Shimizu J, Takaki M, Suga H: "Effects of Ca^<2+> and epinephrine on Ca^<2+> recirculation fraction and total Ca^<2+> handing in canine left ventricles." Japanese Journal of Physiology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Takaki M, Matsubara H, Araki J, Zhao LY, Ito H, Yasuhara S, Fujii W, Suga H: "Sasayama S(Ed:)New Horizons for Failing Heart Syndrome" Springer-Verlag Tokyo, 273 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suga H, Yamaguchi H, Araki J, Suzuki S, Shimizu J, Matsubara H, Kawaguchi O, Yaku H, Takaki M: "Akutsu T, Koyanagi H(Eds:) Heart Replacement : Artificial Heart 6" Springer-Verlag Tokyo, 521 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shimizu J,Takaki M,Kohno K,Araki J,Matsubara H,Suga H: "Sinusoidal and exponetial decays of postextrasystolic transient alternans in excised blood-perfused canine hearts." Japanese Journal of Physiology. 45. 837-848 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsushita T,Takai M,Fujii W,Matsubara H,Suga H: "Lett ventricular mechanoenergetic under altered coronary perfusion in guinea pig hearts." Japanese Journal of Physiology. 45. 991-1004 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Zhao LY,Takaki M,Fujii W,Ishioka K,Suga H: "Discrepancy between left ventricular mechanoenergetics and mitochondrial respiratory function in canine acute failing hearts." Pathophysiology. 2. 211-216 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuhara S,Takaki M,Kikuta A,Suga H: "Myocardial Vo_2 of mechanically unloaded contraction of rat ventricular slices measured by a new approach." American Journal of Physiology. 270. H1063-H1070 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi H,Takaki M,Matsubara H,Yasuhara S,Suga H: "Constancy and variability of contractile effciency as a function of calcium and cross-bridge kinetics : simulation." American Journal of Physiology. 270. H1501-H1508 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ito H,Takaki M,Yamaguchi H,Tachibana H,Suga H: "Left ventricular volumetric conductance catheter for rats." American Jpurnal of Physiology. 270. H1509-H1514 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takaki M,Nakahara H,Kawatani Y,Utsumi K,Suga H: "No Suppression of respiratory function of mitochondria isolated from hearts of anesthetized rats with high-dose pentobarbarbital sodium." Japanese Journal of Physiology. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Tachibana H,Takaki M,Lee S,Ito H,Yamaguchi H,Suga H: "New mechanoenergetic evaluation of left ventricular contractility in in situ rat hearts." American Journal of Physiology. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Takaki M,Matsubara H,Araki J,Zhao LY,Ito H,Yasuhara S,Fujii W,Suga H: "Sasayama S(Ed:) New Horizons for Failing Heart Syndrome" Springer-Veriag Tokyo, 273 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shimizu J, Takaki M, Kohno K, Araki J, Matsubara H, Suga H: "Sinusoidal and exponential decays of postextrasystolic transient alternans in excised blood-perfused canine hearts." Jpn J Physiol. 45. 837-848 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasuhara S, Takaki M, Kikuta A, Ito H, Suga H: "Myocardial Vo_2 of mechanically unloaded contraction of rat ventricular slices measured by a new approch." Am J Physiol. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ito H, Takaki M, Yamaguchi H, Tachibana H, Suga H: "Left ventricular volumetric conductance catheter for rats." Am J Physiol. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takaki M, Matsubara H, Araki J, Zhao LY, Ito H, Yasuhara S, Fujii W, Suga H: "Sasayama S (Ed.) New Horizons for Failing heart Syndrome" Springer-Verlag Tokyo, 273 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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