| Project/Area Number |
07508005
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Waseda University |
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Principal Investigator |
TSUCHIDA Eishun Waseda University, School of Science and Engineering, Professor, 理工学部, 教授 (90063461)
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| Co-Investigator(Kenkyū-buntansha) |
SUEMATSU Makato Keio University, School of Medicine, Associate Professor, 医学部, 助教授 (00206385)
ISHIMURA Yuzuru Keio University, School of Medicine, Professor, 医学部, 教授 (40025599)
KOBAYASHI Kouichi Keio University, School of Medicine, Professor, 医学部, 教授 (80051704)
TAKEOKA Shinji Waseda University, School of Science and Engineering, Associate Professor, 理工学部, 助教授 (20222094)
NISHIDE Hiroyuki Waseda University, School of Science and Engineering, Professor, 理工学部, 教授 (90120930)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 1996: ¥12,000,000 (Direct Cost: ¥12,000,000)
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| Keywords | red cell substitute / hemoglobin / hemoglobin vesicle / microcirculation / animal test / oxygen transport / oxygen dynamics / liver perfusion test / 血流動態 / 肝灌流試験 / リン脂質小胞体 |
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| Research Abstract |
This research is aimed to evaluate two kinds of blood substitutes in preclinical stage (A.totally synthetic heme and B.hemoglobin (Hb) vesicles) in microcirculation levels, changing the properties of those oxygen carriers and monitoring oxygen distribution in tissues by new apparatus for quantitative analyzes.
In the 90% exchange transfusion tests of Hb vesicles in a 5% albumin solution using rats, stable blood gas parameters and tissue oxygen tension were observed during the entire experiment with almost perfect survival rate, indicating excellent oxygen transporting ability and high safety. The Hb vesicles modified with polyoxyethylene (Mw.5kD) was dispersed in an isotonic albumin solution, and the 90% exchange transfusion ws carried out with hamster whose back was equipped with dorsal skinfold chamber to visualize microvessels in the subcutaneous tissue. Microvascular diameter, blood velocity, functional capillary density, microvascular and interstitial oxygen tensions were measured to evaluate the microcirculatory dynamics of the modified Hb resicles.
Tissue oxygenation of isolated perfused liver was measured quantitatively from the intensity of NADH autofluorescence in periprotal and pericentral regions of the hepatic parenchyma. The excellent properties of cellular Hb compared with acellular one and the effect of surface modification were confirmed from the measurements of microcirculation dynamics.
The totally synthetic heme oxygen carriers also showed oxygen binding ability similar to red blood cells in both in vitro and in vivo measurements. Those fruits will be necessarily to apply those materials in clinical trials in near future.
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