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Developmental Studies of Image Analizing System for Animal Karyotype

Research Project

Project/Area Number 07508006
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Laboratory animal science
Research InstitutionNagoya University

Principal Investigator

ODA Sen-ichi  School of Agricultural Sciences, Nagoya University, Associate Professor, 農学部, 助教授 (60023660)

Co-Investigator(Kenkyū-buntansha) YU Ei  The Sumitomo Metal Co.Head Researcher, 研究主任
HASEGAWA Akiumi  Computation Center, Nagoya University, Associate Professor, 大型計算機センター, 助教授 (20126890)
SONTA Shin-ichi  Aichi Prefectural Colony Handicapped for the Mentally and Physically People, Ins, 発達障害研究所, 室長 (00100165)
NAMIKAWA Takao  School of Agricultural Sciences, Nagoya University, Professor, 農学部, 教授 (70111838)
OBARA Takayoshi  School of Sciences, Hirosaki University, Professor, 理学部, 教授 (90003673)
松田 洋一  名古屋大学, 農学部, 助教授 (70165835)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥8,600,000 (Direct Cost: ¥8,600,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsImage analyzing / Karyotype / Chromosome / Banding pattern / 画像処理 / カリオ ビジョン / 核型
Research Abstract

The automation of chromosome analysis is a classific example of contexed -conditioned pattern recognition. During the last years, several systems have been developed to enable automatic karyotyping of human chromosomes. We developed the application software of a image analyzing system for animal karyotypes based on one of these systems for human one, the Karyovision CA2000K from The Sumitomo Metal Co Ltd. Developmental studies were carried out during 3 years as following : 1) We built our net work of computer machine for development of image analyzing system for animal karyotype. We changed a managing sheet or data sheet from for human to animal. In that case it is important to record species' name, collector' name, location of collection and date of collection in wild animals, and species' name, strain's name, and holder or breeder's name in laboratory animals. 2) Using our image analyzing system, we applied many species following as ; Karyograms of Chinese hamsters and house musk shrews, which have all types of chromosomes such as human were recognized well. We also input and analize domestric animals such as pig, goat and cattle, and wild animals such as field vole, shrew-mole so on. It is difficult to recognize karyograms of goat and cattle. Recognition frequencies were between 94,5% in chinese hamster and 67,55 in cattle. 3) We stored freezing cells from animals to supply and to analyze these karyotypes. Using these samples we can develop a system with a better recognizing abilities. We also established standard karyotypes of these animals by banding methods of chromosomes, and we developed new strains with chromosomal deviations, such as translocations and inversion in the Chinese hamster and Robertsonean translocation in the shrew. 4) We took digital images of chromosomes to analyze directly and automatically through a microscope.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Fujimoto, K: "Comparafive analysis of the Karyotype of the greater long-tailed hamster" Cytologia. 62. 315-321 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Obara, Y: "Furuorescent approaches on the origin of B chromosomes of Apodemus argenteus" Chromosomal Scinces. 1. 1-5 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Rogatchera, M: "Robertsonian Chromosomal variation in the house much shen,Suncus murinus and the colonization history of the species" genome. 40. 18-24 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Rogatchera, M: "An xxY sex chromosome condition in the house music shrew with testicular hypoplasia" J. Reproduction and Fertility. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] FUJIMOTO Kazunori, ODA Sen-ichi, KOYASU Kazuhiro, HARADA Masashi and SONTA shin-ichi: "Comparative analysis of the karyotypes of the greater long-tailed hamster and the Chinese hamster" Cytologia. 62. 315-321 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] OBARA Yoshitaka, SASAKI Sanae and IGARASHI Yu: "Delayd response of QM-and DA/DAPI-fluorescence in C-hetero-Cheterochromatin of the small Japanese field mouse, Apodemus argenteus" Zool.Sci.14. 57-64 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] OBARA Yoshitaka and SASAKI Sanae: "Fluorescent approaches on the origin of B chromosomes of Apodemus argenteus hokkaidi" Chrom.Sci.1. 1-5 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] ROGATCHEVA Margarita B., BORODIN Pavel M.and ODA Sen-ichi, and SEARLE Jeremy B.: "Robertsonian chromosomal variation in the house musk shrew (Suncus murinus, Insectivora ; Soricidae) and the colonization history of the species" Genome. 40. 18-24 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] ROGATCHEVA Margarita B., SERDYUKOVA Nataly A., BILTUEVA Larisa S., PERELMAN 'Polina L., BORODIN Pavel M., ODA Sen-ichi and GRAPHODATSKY Alexander S.: "Localization of the genes for major ribosomal RNA on chromosomes of the house musk shrew, Suncus murinus, at meiotic and mitotic cells by fluolescence in situ hybridization and silver staining" Genes and Genetic Systems. 72. 215-218 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] RUBTSOV N., SERDYUKOVA Nataly A., KAFTANOVSKAYA E., YANG F., BILTUEVA Larisa S., VOROBIEVA N., ROGATCHEVA Margarita B,ODA Sen-ichi, and GRAPHODATSKY Alexander S.: "Visualization of the cattle Xp homologous regions on the X chromosomes of some pecorans by chromosome microdissection and heterologous painting" Cytologia. 62. 203-208 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] YANG,J.-P., ONO,T., SONTA,S., KAWABE,T., OKAMOTO,T.: "Assignment of p53 binding protein (TP53BP2) to human chromosome band 1q42.1 by in situ hybridization" Cytogenet.Cell Genet.78. 61-62 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] ROGATCHEVA Margarita B., ODA Sen-ichi, ZHELEZOVA Antonina I., and BORODIN Pavel M.: "An XXY sex chromosome constitution in the house musk shrew (Suncus murinus L.) with testicular hypoplasia" Journal of Reproduction and Fert ility. (accepted). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujimoto,K: "Comparative analysis of the karyotype of the greater long-tailed hamster" Cytologia. 62. 315-321 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Obara,Y: "Furuorescent approaches on the origin of B chromosomes of Apodemus argenteus" Chromosomal Sciences. 1. 1-5 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Rogatcheva,M.: "Robertsonian chromosomal variation in the house mush shrew,suncus murinus and the colonization history of the species" genome. 40. 18-24 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Rogatcheva,M.: "An XXY sex chromosome condition in the house mush shrew with testicular hypoplasia" J.Reproduction and Fertility. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Regatcheva et al: "Standard Karyotype of the house musk shrew,Suncus murinus" Cytologia. 61. 197-208 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki et al: "Rat 55 r DNA spacer sequences and chromosomal assignment." Cytogenet,Cell.Genet. 72. 1-4 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuda et al: "Chromosome mapping of the GD3 sgn thase gene in man and mouse." Genomics. 32. 137-139 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masuda et al: "Comparative mapping of the reeler gene" Mammal Genome. 7. 468-469 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuda et al: "Comparative genome mapping of ataxia telangiectasia" Genomics. 34. 347-352 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kawamoto et al: "Establishment and karyological analysis of embryonic stem cell" Chromosome info serv. 60. 15-17 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 孫田信一: "染色体診断の基本原理と検査技術,生殖細胞" 金原出版(東京), 269-273 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] OBARA Takayoshi: "Revised Karyotypes of the Japanese northern red-backed vole" J.Mamm.Soc.Japan. 20. 125-133 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] SONTA Shin-ichi: "Independent chromosome segregation" Cytogenet Cell Genet. 71. 380-384 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] SONTA Shin-ichi: "Meiotic crossing-over on the interstitial segments" Chrom.Infor.Ser.58. 4-7 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] MATSUDA Yoichi: "Application of fluores cence in situ hybridization" Electrophoresis. 16. 261-272 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] BORODIN Pavel: "Analysis of synaptonemal complexes behavior" Cytologia. 60. 261-272 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 藤本和則: "染色体バンド法によるトリトンハムスターの核型分析" 環研年報. 46. 201-204 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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