An experimental investigation of locomotive behavior using an automated monitor system in the mouse and the rat : Effects of aging and NMDA antagonist treatment

Research Project

Project/Area Number	07551002
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	試験
Research Field	実験系心理学
Research Institution	Shiga Univ.
Principal Investigator	SEKIGUCHI Shigehisa Shiga Univ. Faculty of Education Prof., 教育学部, 教授 (40024905)
Co-Investigator(Kenkyū-buntansha)	KOMADA Noriko Shiga Univ. Faculty of Education Associate Prof., 教育学部, 助教授 (50127007)
Project Period (FY)	1995 – 1996
Project Status	Completed (Fiscal Year 1996)
Budget Amount *help	¥4,400,000 (Direct Cost: ¥4,400,000) Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000) Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywords	mouse / rat / open-field behavior / exploratory activity / aging / MK801 / 行動発達自動解析装置 / C57BLマウス / 移動区画数 / 立ち上がり回数 / Sprague-Dawleyラット
Research Abstract	In Exp.1, age-sex related differences of the C57BI/6J mouse were studied in an open-field behavior. Total quaantities of locomotion, rearing and defecation were all found to differ significally between male and female mice of the older ages. The absolute rate of habituati on and the frequency of face-washing were found also to differ significantly as a function of aging. The possibility of age-sex related differences of the open-field behavior in the system of neuroendocrine is discussed. In Exp.2, rats given NMDA antagonist (MK801) were used in the open-field for three days. Male and female rats were injected ipwith either low dose (0.05mg/kg), middle dose (0.10mg/kg), high dose (0.20mg/kg) or equivalent volume of saline before testing of Day 2. The animal was placed individually in the field and was videotaped for 10-min period on three successive days. On the hige dose of MK801 induced hyperactivity of male rats. However, the high does of MK801 impaired locomotion and exploratory activity in female rats. In the female rats, indeed, the middle and high doses produced mild ataxi and head weaving in addition to the activity deficites. These results, together with the finding from previous experiment used with mice, further suggest that open field behavior and exploratory activity require NMDA-dependent synaptic plasticity