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Development of an Ultraviolet Raman Microspectrometer Applicable to Structural Analyzes of Cells

Research Project

Project/Area Number 07554025
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Physical chemistry
Research InstitutionTohoku University

Principal Investigator

TAKEUCHI Hideo  Tohoku University, Faculty of Science, Department of Pharmaceutical Sciences Professor, 薬学部, 教授 (30111454)

Co-Investigator(Kenkyū-buntansha) TOYAMA Akira  Tohoku University, Faculty of Science, Department of Pharmaceutical Sciences Ins, 薬学部, 助手 (60217560)
HASHIMOTO Shinji  Tohoku University, Faculty of Sciences Department of Pharmaceutical Science, Ins, 薬学部, 助手 (50192696)
MIURA Takashi  Tohoku University, Faculty of Sciences Department of Pharmaceutical Science, Ass, 薬学部, 講師 (30222318)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥15,900,000 (Direct Cost: ¥15,900,000)
Fiscal Year 1996: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1995: ¥12,200,000 (Direct Cost: ¥12,200,000)
KeywordsMicroscope / Protein / Nucleic acid / Cell structure / UV resonance Raman spectroscopy
Research Abstract

Raman microspectroscopy is a useful technique to investigate the structures of micrometer-sized particles in a non-destructive manner. On the other hand, ultraviolet resonance Raman spectroscopy provides key information on the structures of proteins and nucleic acids. In this study, we have developed an ultraviolet Raman microspectrometer to exploit the utilities of both spectroscopic methods. The microspectrometer consists of a continous-wave ultraviolet laser, an ultraviolet microscope, and a Raman spectrometer. The ultraviolet laser beam is obtained by frequency-doubling visible laser light from an Ar^+ laser. The ultraviolet microscope is specially designed for high throughput in the ultraviolet region and high spatial resolution of microscopic images. Samples under a microscope objective are irradiated with ultraviolet laser light and the Raman scattered light was collected with the same objective and then guided to the Raman spectrometer, which has already been developed in our laboratory. We have succeeded to record some Raman spectra by using newly developed ultraviolet Raman microspectrometer.
However, the sensitivity of the microspectrometer is not enough to investigate the structures of microparticles in living cells. We are planning to use an ultra-high-sensitivity CCD detector to detect even extremely weak Raman light and to apply the ultraviolet Raman microspectrometer to structural analyzes of living cells.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report

Research Products

(3 results)

All Other

All Publications (3 results)

  • [Publications] Yoshiaki Takai: "Lipid Structure of Cytotoxic Granules in Living Human Killer T Lymphocytes Studied by Raman Microspectroscopy" Biochim.Biophys.Acta. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshiaki Takai, Takashi Masuko, and Hideo Takeuchi: "Lipid Structure of Cytotoxic Granules in Living Human Killer T Lymphocytes Studied by Raman Microspectroscopy" Biochim.Biophys.Acta. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshiaki Takai: "Lipid Structure of Cytotoxic Granules in Living Human Killer T Lymphocytes Studied by Raman Microspectroscopy" Biochim. Biophys. Acta. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1995-03-31   Modified: 2016-04-21  

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