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Preparations and applications of novel copolymeric adsorbents for selective removal of endotoxin

Research Project

Project/Area Number 07555294
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 高分子合成
Research InstitutionKumamoto University

Principal Investigator

HIRAYAMA Chuichi  Kumamoto University, Applied Chemistry, Professor, 工学部, 教授 (10040429)

Co-Investigator(Kenkyū-buntansha) MORIMOTO Sunao  Kohjin Co., Ltd., Fiber & Pulp Division, General Manager, 開発技術本部, 部長
SAKATA Masayo  Kumamoto University, Applied Chemistry, Assistant Professor, 工学部, 助手 (60187391)
伊原 博隆  熊本大学, 工学部, 助教授 (10151648)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥4,300,000 (Direct Cost: ¥4,300,000)
Keywordsendotoxin / lipopolysaccharide / N,N-dimethylaminopropylacrylamide / divinylbenzene / bovine serum albumin / Escherichia coli / amino-group content / molecular mass exclusion / パイロジェン / N, N-ジメチルアミノプロピルアクリルアミド / アリルアクリルアミド / N_1N-ジメチルアミノプロピルアクリルアミド / 細孔径 / ポアサイズ / イオン的相互作用
Research Abstract

Novel copolymeric adsorbents for selective removal of endotoxin from an acidic protein solution were prepared. The adsorbents comprise spherical copolymers derived from N,N-dimethylaminopropylamide (DMAPAA) and divinylbenzene (DVB). When the molar ratio of DMAPAA to DVB was 80/20 (amino-group content : 5.1 meq/g) and the pore size (molecular mass exclusion of polysaccharide, M_<lim>) was 4000 to 10000, DMAPAA/DVB showed a high endotoxin-adsorbing activity at pH 5.0 to 9.0 and ionic strengths of mu = 0.05 to 0.4. The capacity of the adsorbent (M_<lim> : 4,000) was 390 mg of endotoxin (lipopolysaccharide purified from E.coli O111 : B4) per ml of the adsorbent in the batchwise method. The apparent dissociation constant between endotoxin and the adsorbent was 2.2 X 10-12 M.On the other hand, the adsorption of bovine serum albumin, an acidic protein, by the adsorbent increased with increase in Mlim from 4,000 to 10,000, but decreased with increase in the ionic strength (mu) from 0.05 to 0.2. As a result, DMAPAA/DVB (80/20) (M_<lim> : 4,000) selectively removed endotoxin from various acidic protein solutions at pH 7.0 and m = 0.05. The residual concentration of endotoxin in the protein solution always decreased to a concentration lower than 0.1 ng/ml and the recovery of the protein was over 97%.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] S.Morimoto, M.Sakata, T.Iwata, A.Esaki, C.Hirayama: "Preparations and applications of polyethylene-immobilized cellulose fibers for removal of endotoxin." Polymer Journal. 27・8. 839-841 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Sakata, T.Sueda, H.Ihara, C.Hirayama: "Partially-hydrophobized polymer particles derived from N,N-dimethylaminopropylacrylamide for endotoxin removal from acidic protein solution." Chemical & Pharmaceutical Bulletine. 44・2. 328-332 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 平山 忠一、伊原 博隆: "高分子分離剤の機能設計と分子設計" 高分子. 46. 870-873 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.Hirayama, H.Ihara, S.Nagaoka, M.Sakata: "Poly (α-amino acid) spherical particles." Polymeric materials encyclopedia,. 7. 5444-5455 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Sakata, T.Sueda, H.Ihara, C.Hirayama: "Fronteers in Biomedicine and Biotecnology,Vol.3," ATL Press U,S,A., 10 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 伊原 博隆, 平山 忠一, 鉢迫 博, 山田 仁穂: "配向性分子材料の機能設計と用途新展開" アイピーシー, 288 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sunao Morimoto, Masayo Sakata, Toru Iwata, Akihiko Esaki, C.Hirayama: "Preparations and applications of polyethylene-immobilized cellulose fibers for removal of endotoxin." Polymer Journal. Vol.27, No.8. 839-841 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirotaka Ihara, Chuichi Hirayama, Hiroshi Hachisako, Kimiho Yamada: "Functions and recent applications of highly-orienting Materials" IPC. 1-288 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masayo Sakata, Takanori Sueda, Hirotaka Ihara, Cuichi Hirayama: "Partially-hydrophobized polymer particles derived from N,N-dimethylaminopropylacrylamide for endotoxin removal from acidic protein solution." Chemical & Pharmaceutical Bulletine. Vol.44, No.2. 328-332 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chuichi Hirayama, Hirotaka Ihara: "Biomimetics in high performance liquid chromatography." High Polymers, Japan. Vol.46. 870-873 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masayo Sakata, Takanori Sueda, Hirotaka Ihara, Chuichi Hirayama: Fronteers in Biomedicine and Biotecnology, Vol.3, Reduction of endotoxin from albumin solution with crosslinked N,N-dimethylaminopropylacrylamide particles.ATL Press, 270-278 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chuichi Hirayama, Hirotaka Ihara, Shyoji Nagaoka, Masayo Sakata: Polymeric materials encyclopedia.Vol.7, Poly (alpha-amino acid) spherical particles.CRC Press, U.S.A., 5444-5455 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 平山忠一、伊原博隆: "高分子分離剤の機能設計と分子設計" 高分子. 46. 870-873 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 平山忠一: "Partially-hydrophobized polymer particles derived from N,N-dimethylamino-propylacrylamide for endotoxin removal from acidic protein solution" Chemical & Pharmaceutical Bulletin. 44. 328-332 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 坂田眞砂代: "Fronteers in Biomedicine and Biotecnology" ATL Press,Inc.Science Publishers,U.S.A., 9 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 坂田 眞砂代: "Reduction of endotoxins from albumin solution with cross] linked N,N-dimethyl-aminopropylacrylamide Particles" Proceedings of 1995 International Chemical congress of Pacific Basin Societies. 519 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 平山 忠一: "Partially-hydrophobized polymer particles derived from N,N-dimethylamino-propylacrylamide for endotoxin removal from acidic protein solution" Chemical & Pharmaceutical Bulletin. 44. 328-332 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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