• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of Fermentation Control Systems Applying Artificial Intelligent Techniques

Research Project

Project/Area Number 07555562
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section試験
Research Field 生物・生体工学
Research InstitutionOsaka University

Principal Investigator

YOSHIDA Toshiomi  Osaka University, International Center for Biotechnology, Professor, 生物工学国際交流センター, 教授 (00029290)

Co-Investigator(Kenkyū-buntansha) NAKAJIMA Mikio  Osaka University, department of Biotechnology, Research assistant, 工学部, 助手 (00273590)
KISHIMOTO Michimasa  Osaka University, department of Biotechnology, Associate Professor, 工学部, 助教授 (00144436)
FUJIYAMA Kazuhito  Osaka University, International Center for Biotechnology, Research assistant, 生物工学国際交流センター, 助手 (70209112)
TAKAGI Mutsumi  Osaka University, International Center for Biotechnology, Research assistant, 生物工学国際交流センター, 助手 (20263212)
SEKI Tatsuji  Osaka University, International Center for Biotechnology, Professor, 生物工学国際交流センター, 教授 (50029245)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
KeywordsFed-batch culture / Recombinant / Artificial Neural network / State estimation / Physiological state variables / Expert system / Bio-processes / Man-machine interface / 計算機制御 / 人工知能的手法 / 遺伝子操作 / フェニールアラニン / 最適化 / ファジィ
Research Abstract

The research was made for the development of an intelligent control system for industrial application based on the previous research results on the application of artificial intelligent techniques on the control system aiming construction of a computer control system for microbial processes. The process of phenylalanine production by a recombinant Escherichia coli AT2455 harboring a vector plasmid of a temperature sensitive gene expression system was studied to develop a software for the optimum control based on the state estimation of processes by use of the fuzzy theory, artificial neural networks and expert systems being supported by the preliminary investigation on cultivation of the organism.
The investigation on the control of feeding of tyrosine for a heterotrophic gene-engineered Escherichia coli, AT2455 for the production of phenylalanine revealed that the feeding of tyrosine enhanced growth and the production of phenylalanine and that over feeding reduced the yield of the production. A software for the state estimation applying the artificial neural network techniques for the computer control. For the identification of physiological state a diagnostic program was developed investigating the approaches to detect the transient of physiological state and to control processes accordingly. A control system was established to maintain the desired physiological state processing the information obtained during cultivation using a algorithm of state estimation and control.
A integrated man-machine interface was developed to make an adequate action on spontaneous cultivation involving heavy metal

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Mutsumi Takagi et al: "Control of L-phenylalanine production by dual feeding of glucose and L-tyrosine" Biotechnology and Bioengineering. 52. 653-660 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuyoshi Suzuki et al: "A novel Man-Machine Interface for a Bioprocess Expert system Constructed for Cooperative Decision making and Operation" Journal of Biotechnology. 52. 277-282 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wiwat Ruenglertpanyakul: "Application of Neural Networks to Variables Estimation and Stage Identification in L-Phenylalanine Production" Biotechnology and Bioengineering. (in preparation).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ricaredo M.Matanguihan: "Detection and Control of Physiological State Variability in Continuous Cultivation of a Recombinant Bacteria" Biotechnology and Bioengineering. (in preparation).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mutsumi Takagi et al.: "Control of L-Phenylalanine Production by Dual Feeding of Glucose and L-Tyrosine" Biotech.Bioeng.52. 653-660 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuyoshi Suzuki et al.: "A novel Man-Machine Interface for a Bioprocess Expert system Constructed for Cooperative Decision making and Operation" J.Biotechnology. 52. 277-282 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wiwat Ruenglertpanyakul et al.: "Application of Neural Networks to Variables Estimation and Stage Identification in L-Phenylalanine Production" Biotech.Bioeng.(in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ricaredo M.Matanguihan et al.: "Detection and Control of Physiological State Variability in Continuous Cultivation of a Recombinant Bacteria" Biotech.Bioeng.(in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi