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Studies on the Development of Selectively Modified Cyclodextrin Stationary Phases for HPLC

Research Project

Project/Area Number 07555568
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 工業分析化学
Research InstitutionOsaka University

Principal Investigator

TANAKA Minoru  Osaka Univ., Res. Center Environ. Preserv., Professor, 保全科学研究センター, 教授 (60029238)

Co-Investigator(Kenkyū-buntansha) YOSHINAGA Masanobu  Toppan Printing Co., Kansai Research Institute, Researcher, 関西研究所, 研究員
YASAKA Yuta  Osaka Univ., Res. Center Environ. Preserv., Assistant Professor, 保全科学研究センター, 講師 (90150333)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsLiquid chromatography / Chemically modified cyclodextrin stationary phases / Chiral separation / Dansylamino acid derivatives
Research Abstract

The method to isolate selectively methylated beta-and gamma-cyclodextrin (CD) derivatives could be established. Their hydroxyl groups at 2-position (2-MM-) 3-position (3-MM-), both 2- and 3-positions (2,3-DM-) and 2-, 3-and 6-positions (2,3,6-TM-) were methylated. The mono-, di- and trimethylated CD derivatives are donared by prefixing the unmodified CD with MM-, DM- and TM-, respectively.
After monoallyloxyethylation, the unmodified and methylated CDs were treated with dimethoxymethylsilane, followed by coupling on the silica gel surface. Selectively methylated CD-bonded stationary phases for HPLC without an unreacted spacer arm could be thus obtained. This preparation method of the CD stationary phases brought about a ready estimation of the precise amount of CD immobilized and easy immobilization of the gamma-CD derivatives as well as the beta-CD ones.
The chiral recognition abilities of the resulting gamma-CD stationary phases were evaluated using dansylamino acids as model solutes. Unmodified gamma-CD and 3-MM-gamma-CD stationary phases could resolve most of their enantiomers, while 2-MM-gamma-CD and 2,3,6-TM-gamma-CD stationary phases could not at all. These results positively suggest an important contribution of the 2-hydroxyl group to the chiral recognition for these solutes. It is found that the selectively methylated gamma-CD stationary phases exhibit the high enantioselectivities as expected. Similar investigation is now in progress on the selectively methylated beta-CD stationary phases.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Tanaka, Minoru: "Preparation and Retention Behavior of Cyclodextrin-Bonded Stationary Phases on a Silica Support without an Unreacted Spacer Chain" Analytical Sciences. 11. 227-231 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshinaga, Masanobu: "Enantiomeric Separation of Dansylamino Acids by Capillary Electrophoresis with Selectively Methylated γ-Cyclodextrin Derivatives" Analytica Chimica Acta. 316. 121-127 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miura, Masaru: "Chiral Separation of Several Naphthalenesulfonylamino Acids by Capillary Zone Electrophoresis with Selectively Methylated Cyclodextrin Derivatives." Analytica Chimica Acta. 357. 177-185 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka, Minoru: "Preparation and Retention Behavior of Cyclodextrin-Bonded Stationary Phases on a Silica Support without an Unreacted Spacer Chain" Analytical Sciences. 11. 227-231 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshinaga, Masanobu: "Enantiomeric Separation of Dansylamino Acids by Capillary Electrophoresis with Selectively Methylated gamma-Cyclodextrin Derivatives" Analytica Chimica Acta. 316. 121-127 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miura, Masaru: "Chiral Separation of Several Naphthalenesulfonylamino Acids by Capillary Zone Electrophoresis with Selectively Methylated Cyclodextrin Derivatives." Analytica Chimica Acta. 357. 177-185 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miura,Masaru: "Chiral separation of several naphthalenesulfonylamino acids by capillary zone electrophoresis with selectively methylated cyclodextrin derivatives." Analytica Chimica Acta. 357. 177-185 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanaka,Minoru: "Preparation and Retention Behavior of Cyclodextrin-Bonded Stationary Phases on a Silica Support without an Unreacted Spacer Chain" Analytical Sciences. 11. 227-231 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshinaga,Masanobu: "Enantiomeric Separation of Dansylamino Acids by Capillary Electrophoresis with Selectively Methylated γ-Cyclodextrin Derivatives" Analytica Chimica Acta. 316. 121-127 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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