| Project/Area Number |
07556002
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
Breeding science
|
|---|
| Research Institution | Iwate University |
|---|
Principal Investigator |
TAKAHATA Yoshihito Iwate Univ., Fac.Agr.Professor, 農学部, 教授 (10133894)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KUGINUKI Yasuhisa Nati.Res.Inst.Vege., Orn.Plants, Tea, Senior Researcher, 野菜・茶業試験場, 主任研究員
YAMAGISHI Hiroshi Kyoto Sangyo Univ., Fac.Eng.Associate Professor, 工学部, 助教授 (10210345)
KANEKO Yukio Utsunomiya Univ., Fac.Agr.Associate Professor, 農学部, 助教授 (40241848)
KATO Masahiro Ehime Univ., Fac.Agr.Professor, 農学部, 教授 (30036404)
OHKAWA Yasunobu Natl.Inst.Agrobiol.Resourc.Head, 生物資源研究所, 室長
MINAMI Harufumi Tokyo Metro.Indus.Tec.Res.Inst., Senior Staff Member
|
|---|
| Project Period (FY) |
1995 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1996: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1995: ¥5,600,000 (Direct Cost: ¥5,600,000)
|
|---|
| Keywords | Brassica / Raphanus / Microspore culture / Haploid method of breeding / Mutation / Transformation / 形質転換 / Brissica |
|---|
| Research Abstract |
1.Practical breeding : The DH lines, which had both high microspore embryogenesis ability and characteristics similar to Japanese cultivars, have been developed using microspore culture in Chinese cabbage and cabbage. The ABA-treated embryos sprouted in pots under unsterile conditions after 1 year storage at 4ﾟC.This storage system was expected to be useful for haploid breeding.
2.Mechanism : The androgentic embryos were clarified to be heterotrophic at early developmental stage, but autotrophic after heart-shaped stage. Inheritance of microspore embryogenesis was studied by 4x4 diallel analysis. High embryogenesis ability was dominant to low ability, and additive gene effect was predominant. The heritability for embryogenesis was quite high. RAPD analysis showed that 3 RAPD makers were identified to be associated with embryogenesis.
3.Enlargement of variation : The effect of UV irradiation on microspore culture was examined. The microspores were highly sensitive to UV.Using in vitro selection of UV irradiated-microspoes by soft rot-and black rot-filtered solution, disease tolerant-mutants of Chinese cabbage were selected. Transgenic B.napus was produced by direct gene transfer and Agrobacterium-mediated method using microspores as targets.
4.Estimation of haploid breeding : The degree of genetic fixation in DH lines was examined by RAPD analysis. Although the DH lines showed much higher uniformity than parental lines, the variation among the individuals still remained.
|
