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Development of an informatics system for quantitative trait locus analysis with the aid of DNA markers

Research Project

Project/Area Number 07556004
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Breeding science
Research InstitutionThe University of Tokyo

Principal Investigator

UKAI Yasuo  The Univ.of Tokyo, Grad.Sch.Agr.Life Sci., Professor, 大学院・農学生命科学研究科, 教授 (80240729)

Co-Investigator(Kenkyū-buntansha) NINOMIYA Seisi  Agri.Res.Center, Dept.Res.Inform.Senior Researcher, 研究情報部, 上席研究官
TAKANO Yasushi  The Univ.of Tokyo, Grad.Sch.Agr.Life Sci., Lecturer, 大学院・農学生命科学研究科, 講師 (40012009)
KISHINO Hirohisa  The Univ.of Tokyo, College Arts Sci., Asso.Professor, 大学院・総合文化研究科, 助教授 (00141987)
HARADA Hisaya  Chiba Univ., Fac.Hort.Professor, 園芸学部, 教授 (70011913)
SAIO Kenjiro  The Univ.of Tokyo, Emer.Professor, 大学院・農学生命科学研究科, 名誉教授 (80011831)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1996: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1995: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsQuantitative trait / QTL / DNA polymorphism / Linkage map / Marker / Genetic effect / Elliptoc Fourie descriptor / Program / 形態
Research Abstract

A computer program MAPL97 for construction of DNA marker linkage map and quantitative trait locus (QTL) analysis which was written on Visual Basic and is available on Win95 system was developed and announced in a home page of web. The program can be used for QTL analysis of allogamous plant species and silkworm F2 in additoion to ordinary F2, BC and DH.Simlulated data can be generated for each type of populations to test the calculation procedures. The program has been effectively utilized for the consturction of the DNA marker linkage map of silkworm and also is widely used for QTL analysis of rice and other plant species. A method for estimation of the mode, degree and position on the linkage map of a lethel factor which leads to distortion of segregation data was developed for BC,DH,and silkworru F2.
A new map function in which an obligatory crossover on a chromosome is taken into account was presented and a good fitness of the map function to the observed data in rice was found. A m … More ethod for effective selection of a quantitative trait which utilizes both information of DNA marker types and the phenotypic value of the quantitative trait was developed. Conditions under which the method is more effective than the one solely based on marker types and ordinary selection method based on phenotypes of a trait were tabulated. Fisher's amount of information for estimate of recombination values were calculated for varying generations of recombinant inbred lines and it was found that a marked increase in the amount of information with generation was found in case of dominant DNA markers such as RAPD.Eespecially in case of repulsion phase, recombinant inbred lines was proven to be very effective for mapping.
When grain shape of barley DH lines was described by Elliptic Fourie descriptor, subjected to principal component analysis and further score of each principal component was evaluated by QTL analysis, several peaks for LOD score graph suggesting the presence of QTL was observed. Quantitative description of whole shape of a growing plant by Fuzzy sets was attempted in soybean. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] 大澤 良 ほか: "Genetic Fixation in an Allogamous plant population with special reference to spatial distribution and restricted pollen flow." Genes&Genetic Systems. 72. 283-290 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 松永 数彦 ほか: "新しいDNAフィンガープリンティングソフトによる解析例" DNA多型. 5. 163-169 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 佐藤 和広 ほか: "Net blotch resistance in the wild species of Hordeum." Euphytica. 95. 179-185 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 二宮 正士 ほか: "WWW圃場画像獲得システム" 植物工場学会誌. 9. 12-19 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 矢野 昌祐 ほか: "Identification of quantitative trait loci controlling heading dateof rice using a high-density linkage map." Theor Appl Genet. 95. 1025-1032 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 矢野 昌祐 ほか: "Genetic and molecular dissection of quantitative traits in rice." Plant Mol.BIOL.35. 145-153 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 程 融 ほか: "Detection and estimation of lethal factors by using a DNA polymorphism linkage map." Theor.Appl.Genet.93. 494-502 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 間野 吉郎 ほか: "Mapping genes for callus growth and shoot regeneration in barley (Hordeum vulgare L.)." Breed.Sci.46・3. 137-142 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 梶矢 弘美 ほか: "Effective production of RFLP markers between closely related varieties of rice by genomic subtraction." Breed.Sci.46・4. 387-391 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chow, S.ほか: "Phylogenetic relationships between tuna species of the genus Thunnus (Scombridae:Teleostei)" J.Mol.Evol.41. 741-748 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 林 正紀 ほか: "マイクロサテエライトモチーフによるダイズのゲノムスキャニング." 明治大学農学部研究報告. 104. 25-30 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 生出 真理 ほか: "ダイズ草姿判別におけるニューラルネットワークモデル." システム農学. 12. 13-20 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ukai, Y.: "A package of computer programs for the statistical analysis of genotype x environment interaction and stability." Breed.Sci.46. 73-79 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Cheng, R.: "Estimation of recombination value in the presence of missclassification of a trait in RFLP mapping." Theor.Appl.Genet.92. 524-531 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Furuya, H.: "Quantitative evaluation of soybean (Glycine max L.Merr.) leaflet shape by principal component scores based on elliptic Fourie descriptor." Breed.Sci.45. 315-320 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamada, M.: "Genetic analysis of fruit ripening time in Japanese persimmon." J.Aner.Soc.Hort.Sci.120. 886-890 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ukai, Y.: "A apckage of computer programs for construction of DNApolymorphism linkage maps and analysis of QTL." Breed.Sci.45. 139-142 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Omori, H.: "Application of the Fuzzy procedures to the analysis of genotype x environment interaction." Agri.Inform.Res.4. 33-42 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Cheng, R.: "Detection and estimation of lethal factors by using a DNApolymorphism linkage map." Theor.Appl.Genet.93. 494-502 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mano, Y.: "Mapping genes for callus growth and shoot generation smid-like DNAs are located within limited chromosomal regions on the rice nuclear genome." Breed.Sci.46. 137-142 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kajiya, H.: "Effective production of RFLP markers between closely related varieties of rice by genomic subtraction." Breed.Sci.46. 387-391 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chow, S.: "Phylogenetic relationships between tuna species of the genus Tunnus (Scombridge : Teleostei)" J.Mol.Evol.41. 741-748 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hayashi, M.: "genome scaninng of soybean by microsatelite motif." Meiji Univ.Fac.Agr.Research Report. 104. 25-30 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oide, M.: "Neural network model in the descrimination of soybean plant shape." System Agri.12. 13-20 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohasawa, R.: "Genetic fixation in an allogamous plant population with special reference to spatial distribution and restricted pollen flow." Genes & Genetic Systems. 72. 283-290 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsunaga, K.: "Analysis of DNA fingerprint by a new program." DNA polymorphism. 5. 163-169 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sato, K.: "Net blotch resistance in the wild species of Hordeum." Euphytica. 95. 179-185 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ninomiya, S.: "Field image acquisition system on WWW." J.SHITA. 9. 12-19 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yano, M.: "Identification of quantitative trait loci controlling heading date of rice using a high-density linkage map." Theor.Appl.Genet.95. 1025-1032 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yano, M.: "Genetic and molecular dissection of quantitative traits in rice." Plant Mol.Biol.35. 145-153 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大澤良ほか: "Genetic fixation in an allogamous plant population with special reference to spatial distribution and restricted pollen flow." genes & Genetic Systems. 72. 283-290 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 松永数彦ほか: "新しいDNAフィンガープリンティングソフトによる解析例" DNA多型. 5. 163-169 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 佐藤和広ほか: "Net blotch resistance in the wild species of Hordeum." Euphytica. 95. 179-185 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 二宮正士ほか: "WWW圃場画像獲得システム" 植物工場学会誌. 9. 12-19 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 矢野昌裕ほか: "Identification of quantitative trait loci controlling heading dateof rice using a high-density linkage map." Theor Appl Genet. 95. 1025-1032 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 矢野昌裕ほか: "Genetic and molecular dissection of quantitative traits in rice." Plant Mol.Biol.35. 145-153 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 程融ほか: "Detection and estimation of lethal factors by using a DNA polymorphism linkage map." Theor.Appl.Genet.93. 494-502 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 間野吉郎ほか: "Mapping genes for callus growth and shoot regeneration in barley(Hordeum vulgare L.)." Breed.Sci.46・3. 137-142 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 梶矢弘美ほか: "Effective production of RFLP markers between closely related varieties of rice by genomic subtraction." Breed.Sci.46・4. 387-391 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Chow,S.ほか: "Phylogenetic relationships between tuna species of the genus Thunnus(Scombridae:Teleostei)" J.Mol.Evol.41. 741-748 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 林正紀ほか: "マイクロサテエライトモチーフによるダイズのゲノムスキャニング" 明治大学農学部研究報告. 104. 25-30 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 生出真理ほか: "ダイズ草姿判別におけるニューラルネットワークモデル." システム農学. 12. 13-20 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鵜飼保雄ほか: "遺伝子型x環境交互作用および安定性の統計的解析のためのコンピュータ・プログラムGEST" Breed. Sci.46. 73-79 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 程融ほか: "Estimation of recombination value in the presence of misclassification of a trait in RFLP mapping" Theor. Appl. Genet.印刷中. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 古田尚也ほか: "Quantitative evaluation of soybean (Glycine max L. , Merr. )leaflet shape by principal component scores based on elliptic Fourie descriptor" Breed. Sci. 45. 315-320 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 山田雅彦ほか: "Genetic analysis of fruit ripening time in Japanese persimmon" J. Amer. Soc. Hort. Sci.120. 886-890 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鵜飼保雄ほか: "DNA多型連鎖地図作成とQTL解析のためのコンピュータ・プログラムMAPL" Breed. Sci. 45. 139-142 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大森宏ほか: "作物の遺伝子型x環境交互作用の解析におけるファジイ手法の適用" 農業情報研究. 4. 33-42 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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