| Project/Area Number |
07556024
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Kobe University |
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Principal Investigator |
OHKAWA Hideo Kobe University, Faculty of Agriculture, professor, 農学部, 教授 (90233048)
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| Co-Investigator(Kenkyū-buntansha) |
YABUSAKI Yoshiyasu Sumitomo Chemical Co., Ltd., Biotechnology Laboratory, senior research associate, 生命工学研究所, 主席研究員
OHBA Katuaki Shionogi & Co., Ltd, ABURAHI Laboratories, section manager, 油日ラボラトリーズ, 課長(研究職)
SAKAI Yu Kyoto University, Faculty of Agriculture, associate professor, 農学部, 助教授 (60089117)
HIMENO Michio Osaka University, Faculty of Agriculture, professor, 農学部, 教授 (10026411)
薮崎 義康 住友化学工業(株), 生命工学研究所, 主席研究員
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Bacillus thuringiensis / Insecticidal protein / transgenic plants / BT.israelensis / BT.aizawai / cryI.C / cryIVA and B / トランスジェニック・タバコ / BT israelensis / BT aizawai / cryVA and cryIVB / cryIAb / ユスリカ / ボウフラ / ハスモンヨトウ |
|---|
| Research Abstract |
The insecticidal proteins from Bacillus thuringiensis var.israelensis, var.aizawai, and other varieties were evaluated for insecticidal activity against diptera and lepidoptera insects.The selected insecticidal proteins and their genes were analyzed by biochemical and genetic engineering methods. It was revealed that insecticidal proteins were injested by insect larvae and processed in midguts to yield active fragments, which reacted with receptors in midguts to stop eating.
Then, the active fragments and the whole proteins were produced in E.coli, yeast, algae and tobacco plants to develop novel approaches for insect control.
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