| Project/Area Number |
07556044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
林産学
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| Research Institution | Ehime University |
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Principal Investigator |
TACHIBANA Sanro Ehime University, Faculty of Agriculture, Professor, 農学部, 教授 (10112319)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Minoru Osaka Organic Chemical Industry Ltd., chief researcher, 研究部, 主任
OKI Tae Ehime University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (20116916)
ITOH Kazutaka Ehime University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (50253323)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | Tissue cultures / Elicitor / Taxus cuspidata var.nana / Taxol / Anti-biotic activity / Callus cultures / Chitoheptaose / Cell suspension cultures / 固定化細胞 / キトオリゴ糖 / 生合成前駆体 / 微生物変換 / ジベレリン |
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| Research Abstract |
Utilization of wood extractives from Kyaraboku were examined.
1.Taxol, a strong anti-tumor agent, in T.cuspidata was produced by callus cultures of Kyaraboku. Amount of production of taxol was increased 5.1 times larger than that of the control (no addition) when chito-oligosaccharides as a elicitor for ehhancement of taxol production were added to the calli. Chitoheptaose was found to be the best elicitor in the chito-oligosaccharides.
2.Taxol was produced by cell suspension cultures of Kyaraboku. Taxol production increased 6.3 times larger than that of the control when chitoheptaose and jasmonic acid were added to the suspension cultures.
3.Taxol was released into the medium during incubation when cultured cells were incorporated with sodium alginate and incubated. The maximum amount of taxol released into the medium was 0.6 mg/L.
4.Taxinine in Kyaraboku and its derivatives have anti-biotic activities against Cochiliobolus miyabeanus, Altenaria kikuchiana, Colletotrichum fragariae, Botryisis cinerea, and Corynespora cassiicola.
5.A trial of preparation of taxol mimic by condensation of the BaccatinIII like compound derived from taxinine and phenylisosserin was conducted. Phenylisoserin, the side chain of taxol, was stereoselectively synthesized from benzaldehyde in a total yield of 0.9%. However, preparation of the BaccationIII like compound from taxinine are now being conducted.
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