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Establishement of genetic resource of plants against multispectrum disease by using peptide sequences

Research Project

Project/Area Number 07556071
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field 生物資源科学
Research InstitutionThe University of Tokyo

Principal Investigator

NUMBA Shigetou  Graduate School of Agriculture and Agricultural Life Science, Professor, 大学院・農学生命科学研究科, 教授 (50189221)

Co-Investigator(Kenkyū-buntansha) 魯 暁云  日本学術振興会, 特別研究員
TANAKA Yoshikazu  Suntory Ltd., Plant Biotechnology Laboratory, Researcher, 基礎研究所, 研究員
ASHIKARI Toshihiko  Suntory Ltd., Plant Biotechnology Laboratory, Section Manager, 基礎研究所, 主任研究員
LU Xiaoyun  Japan Society pf the Promotion of Science, Research fellow
田中 良知  (株)サントリー, 基礎研究所, 研究員
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 1996: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1995: ¥11,500,000 (Direct Cost: ¥11,500,000)
KeywordsTrichovirus genus / Potyvirus family / citrus tatter leaf virus / potato virus Y / azuki bean mosaic virus / tobacco etch virus / multispectrum
Research Abstract

In this project, we have developed our work as follows :
We have established the technique to add several preventing functions such as de-integument, copying, moving between cells or a body, and maturity of virus protein. Further, we have tried to produce a plant which has the newest resistant properties beyond genus of virus, and establish genetic resource of plants against multispectrum disease.
First, we have produced plants which have potato virus Y,azuki bean mosaic virus, tobacco etch virus, citrus tatter leaf virus, and movement protein and coat protein of potato virus T.Then we have analyzed the functions of each protein. Proceedingly, we have produced full length of cDNA clones of citrus tatter leaf virus genome, composed infectious transcribing RNA and established a system to infect plants by inoculation. Under these results, we have introduced a mutation to the cDNA,investigated influences to the function of the protein, and got a lot of informations. Therefore, it would be possible to get significant knowledges to apply various protein to plants and produce plants which have multispectrum resistant properties in the future.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 難波成任: "MLOからファイトプラズマへ-ファイトプラズマの分類" 植物防疫. 50・4. 30-34 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sato,M.,Nanba,S.,Katsuhara,M.,Kawakita,H.,Mitsuhashi,W.,Kawabe,S.: "PCR (Polymerase Chain Reaction) Detection of Phytoplasma in Phloem Sap Collected by Laser Stylectomy" 日本植物病理学会報. 62・2. 177-180 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 難波成任,他: "植物ウイルスの分子生物学" 学会出版センター, 458 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Namba, S.: "Taxonomy of Phytoplasmas" Plant Protection. Vol. 50.4. 30-34 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sato, M., Namba, S., Katsuhama, M., Kawakita, H., Mitsuhashi, W & Kawabe, S.: "PCR (Polymerase Chain Reaction) Detection of Phytoplasma in Phloem Sap Collected By Laser Stylectomy" Annals of the Phytopathological Society of Japan. Vol. 62.2. 177-180 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Namba, S., et al: Molecular Biology of Plant Viruses. Gakkai Shuppan Center, 458 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 難波成任: "MLOからファイトプラズマへ-ファイトプラズマの分類" 植物防疫. 50・4. 30-34 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sato,M.,Namba,S.,Katsuhara,M.,Kawakita,H.,Mitsuhashi,W.& Kawabe,S:"PCR(Polymerase Chain Reaction)Detection of Phytoplasma in Phoem Sap Collected by Laser Stykctony" 日本植物病理学会報. 62・2. 177-180 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 難波成任他: "植物ウイルスの分子生物学" 学会出版センター, 458 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Namba, S.: "Family description: Capillovirus. In Classification and Nomenclature of viruses, VIth ICTV Report." Archives of Virology. Supplementum. 465-467 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Candresse, T., Namba, S. and Martelli, G. P.: "Family description: Trichovirus. In Classification and Nomenclature of viruses, VIth ICTV Report." Archives of Virology. Supplementum. 468-470 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ohira, K., Namba, S., Rozanov, M., Kusumi, T. and Tsuchizaki,T.: "Complete sequence of an infectious full-length cDNA clone of citrus tatter leaf capillovirus; comparative sequence analysis of capillovirus genomes." J. General Virol.76. 2305-2309 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 難波成任: "ファイトプラズマ研究の新展開" 植物防疫. 49. 11-14 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 難波成任: "植物病理学辞典" 養賢堂(岸 國平他編), 15 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 難波成任: "ウイルス学" 朝倉書店(畑中正一編), 11 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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