| Project/Area Number |
07556088
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | University of Tsukuba |
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Principal Investigator |
NAKAHARA Tadaatsu University of Tsukuba, Institute of Applied Biochemistry, Professor, 応用生物化学系, 教授 (80011930)
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| Co-Investigator(Kenkyū-buntansha) |
YUKAWA Hideaki Tsukuba research center, Mitsubishi Chemical co.Group leader, 筑波研究所・生物化学研究室, 室長
NAKAJIMA Toshiaki (KAMBE) University of Tsukuba, Institute of Applied Biochemistry, Assistant professor, 応用生物化学系, 講師 (80241777)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | poly (malic acid) / microbiol production / biodegradable polymer |
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| Research Abstract |
Poly (L-malic acid), PMA,production by the non-growing cells of Aureobasidium sp.strain A-91 was investigated. Reaction conditions were optimized using non-growing cells of strain A-91. The addition of CuSO_4 to the cell preparation medium was found to be effective to prepare active cells for PMA production. The non-growing cells of the strain yielded 80.0g/l PMA during 5 days' incubation at 30ﾟC under optimal conditions (160g/l glucose and 40g/l CaCO_3).
On the other hand, strain A-91 also produces pullulan and polyol lipid, which are useful for chemical industries, by changing some culture conditions. Examinations were done as a purpose of elucidating regulatory mechanism of production of these products. The kind of N-source in culture broth seemed to be important for the polyol lipid production and the initial culture pH was important for PMA production. In addition, sulfur containing amino acid, such as methionine or cysteine, and zinc ion enhanced PMA production. Copper and iron ions were found to enhance pullulan production.
Mutation was performed using strain A-91 and two Met-mutants showed the same productivities as those of parent strain. These strains seemed to be useful for elucidating regulatory mechanism of production of these products.
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