| Project/Area Number |
07556096
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Shizuoka University |
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Principal Investigator |
KAWAGISHI Hirokazu Shizuoka University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70183283)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAMOTO Hideki Kagome Co.Ltd., Research Institute, Head Researcher, 総合研究所, 主任研究員
FURUKAWA Syouei Gifu Pharmaceutical University, Professor, 教授 (90159129)
UEMURA Daisuke Nagoya University, Faculty of Science, Professor, 理学部, 教授 (00022731)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥4,700,000 (Direct Cost: ¥4,700,000)
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| Keywords | Mushroom / Hericium erinaceum / Nerve growth factor / Mycelium / Fruiting body |
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| Research Abstract |
1) Large scale preparation of known active compounds and search for novel active compounds from mushrooms.
Some kinds of mushrooms (10-500kg) were extracted with ethanol. After removing the ethanol, the extracts were partitioned between organic solvents such as ethyl acetate or chloroform and water. The organic solvent-soluble parts were separated by repeated chromatography, giving known active compounds for in vivo assay. In addition, by screening of the activity against some mushroom extracts, it was revealed that some mushrooms promoted NGF-production. As a result, two new compounds, dictyophorine A and B were purified from a mushroom Dictyophora indusiata.
2) Efficient production of active compounds.
Production of erinacines A were examined under various culture conditions. As a results, an efficient condition was found (3g in 100 ml medium).
3) In vivo experiments of hericenones and erinacines.
The promoters of NGF-synthesis, hericenones and erinacines, have been isolated from a mushroom Hericium erinaceum. The in vivo effects of these compounds were examined using some model rats. The valuation of the effects is now in progress.
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