| Project/Area Number |
07556113
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
生物環境
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| Research Institution | Kinki University |
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Principal Investigator |
OHTA Yoshimoto Kinki University Faculty of Biology-Oriented Science and Technology, Department of Biotechnological Science, Professor, 生物理工学部・生物工学科, 教授 (30258058)
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| Co-Investigator(Kenkyū-buntansha) |
AKITA Motomu Kinki University Faculty of Biology-Oriented Science and Technology, Department, 生物理工学科, 講師 (80258061)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | production of seedlings / mass propagation of plant organs / storage organs / bioreactor |
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| Research Abstract |
We attempted to develop a system for commercial production of storage organs of plants using a simplified bioreactor.
Taro (Colocasia esculanta) and potato (Solanum tuberosum) were used as model plants in this study. Taro could be grown under a statical and completely submerged condition in the liquid medium. This indicates that taro can be cultured under the condition that the oxygen is supplied only by diffusion from the medium surface and forced aeration is not always necessary. Because forced aeration is thought to be one of the most complicated operations for large scale culture, elimination of the forced aeration is great advantage in simplifying the system. We designed a rotary drum type bioreactor in which the explants were cultured without forced aeration. Oxygen was supplied passively through a silicone spongy plug. Plants were entangled and fixed on a matrix inside of the bioreactor. By filling the bioreactor with a suitable volume of the liquid medium, plants were intermittently immersed into the medium by rotation. Corms of taro were successfully propagated in this system. Potato microtubers could also be mass propagated efficiently in this system.
Cultured corms of taro were considered not to be mature because the content of storage protein and starch was significantly low. Taro corms could not be stored long term after finishing culture and their acclimatization just after the culture was easy, whereas root formation was not vigorous. The root formation was stimulated by culturing the corms in pure water or antioxidant solutions at the latest week of the culture and this increased the ratio of survived plants during cultivation was promoted.
Our results indicate that the simplified culture system is applicable for mass propagation of corms of taro and microtubers of potato. Possible system for commercial production of the storage organs was discussed.
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