| Project/Area Number |
07556114
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKAHASHI Shin-ichiro (1997) The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (00197146)
小野寺 一清 (1995-1996) 東京大学, 大学院・農学生命科学研究科, 教授 (90012773)
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| Co-Investigator(Kenkyū-buntansha) |
ONODERA Kazukiyo The University of Tokyo, Graduate School of Agricultural and Life Sciences, Prof, 大学院・農学生命科学研究科, 教授 (90012773)
SAWAZAKI Toru The University of Tokyo, Graduate School of Agricultural and Life Sciences, Prof, 大学院・農学生命科学研究科, 教授 (00012047)
HIGUCHI Hiroyoshi The University of Tokyo, Graduate School of Agricultural and Life Sciences, Prof, 大学院・農学生命科学研究科, 教授 (10111486)
HAYASHI Yoshihiro The University of Tokyo, Graduate School of Agricultural and Life Sciences, Prof, 大学院・農学生命科学研究科, 教授 (90092303)
HASEGAWA Atsuhiko The University of Tokyo, Graduate School of Agricultural and Life Sciences, Prof, 大学院・農学生命科学研究科, 教授 (90011923)
高橋 伸一郎 東京大学, 大学院・農学生命科学研究科, 助教授 (00197146)
河本 馨 東京大学, 農学部, 教授 (30011894)
舘 鄰 東京大学, 農学部, 教授 (30011711)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1996: ¥3,600,000 (Direct Cost: ¥3,600,000)
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| Keywords | GARS / AIRS / GART / fused gene / evolution / human chromosome 21 / プリン生合成酵素遺伝子 / 霊長類における進化 |
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| Research Abstract |
Cloning of a human cDNA encoding the trifunctional glycinamide ribonucleotide synthetase (GARS) -aminoimidazole ribonucleotide synthetase (AIRS) -glycinamide ribonucleotide transformylase (GART) enzyme by functional complementation in E.coli and in yeast has been reported previously. In this study, we demonstrates the isolation of a novel cDNA clone encoding only the GARS domain from a human fetal brain cDNA library using the 5' region of the human GARS-AIRS-GART cDNA as a probe. Analysis of the nucleotide sequence reveals that the coding region of this cDNA is identical to that of the GARS domain of the GARS-AIRS-GART gene cDNA.Analysis of the expression patterns of the two transcripts in normal human brains of various ages demonstrates that each is transcribed at different overall levels depending upon age. We concluded that the human GARS-AIRS-GART gene produces the trifunctional enzyme and the human gene encoding only the GARS domain produced the enzyme showing the activity of only GARS.In addition, the connecting region of GARS and AIRS in the GARS-AIRS-GART gene were completely preserved between the primates. On the contrary, in the 3' untranslate region of the GARS gene we observed a few replacements, inserts as well as deletion of nucleic acids between human and other primates. In conclusion, these results strongly suggested that the GARS-AIRS-GART gene is fused from the independent GARS,AIRS,GART genes evolutionally. Using the chromosome mapping techniques, we continuously intend to analyze other important genes for animal resource sciences.
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