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Molecular breeding of crops that highly absorb nitrogen for the purpose ofsaving energy and for the environmental protection.

Research Project

Project/Area Number 07556130
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 生物資源科学
Research InstitutionKyoto Prefectural University

Principal Investigator

TAKEBA Go  Human Environment, Kyoto Prefectural University Professor, 人間環境学部, 教授 (10046500)

Co-Investigator(Kenkyū-buntansha) HAYAKAWA Takahiko  Mitsubishi-Plant Technology Main Researcher, 植物工学研究所, 主任研究員
YAMASHITA Hirofumi  Human Environment, Kyoto Prefectural University Research assistant, 人間環境学部, 助手 (50264750)
TAKAHASHI Eiichi  Biochemistry, Kinki University Professor, 農学部, 教授 (40026414)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsnitrogen fertirizer / eutorophy / glutamine synthetase / rice plant / tobacco / nitrogen absorption / 形質転換
Research Abstract

Transgenic tobacco plants with the cDNA encoding rice glutamine synthetase were createdusing an Agrobacterium-Ti plasmid vector. One of them showed very rapid growth in MSmedium. Transgenic rice plants with the same gene were also created by electroporationmethod. One of 26 transformants greatly absorbed nitrogen from paddy soil, and had 20stems that were three times more than a control plant. These data indicates that thebiotechnique can be used for the purpose of saving nitrogen fertilizer in the field andfor the environmental protection.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Tanaka,A., et al.: "Isolation of chlorophyll a oxygenase which is involved in chlorophyll a formation from chlorophyll a"Proc.Nat.Acad.Sci.USA. 95. 12719-12723 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 竹葉剛、他: "植物のCO2固定のメカニズム"都市緑化技術. 30. 10-13 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamura,N., et al.: "The nitrate reductase gene isolated from DNA of cultured spinach cells"Biochim.Biophys.Acta. 1338. 151-155 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka,O., et al.: "Flower-inducing activity of lysine in Lemma pauciostata 6746"Plant Cell Physiol.. 38. 124-128 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 木崎暁子、他: "植物の陸上化と光呼吸"遺伝. 51. 9-11 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 竹葉剛: "省施肥・ストレス耐性植物の育成"バイオサイエンスとインダストリー. 55. 35-36 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeba,G、 他: "Photorespiration is an essential mechanism for the protection C3 plants from photooxidation. In Stress Responses of Photosynthetic Organisms"Elsevir Amsterdam. 22 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka,A. et al.: "Molecular Biology to Biotechnology"Redox reactionss in the last steps of chlorophyll biosynthesis in the chloroplast. 6 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kozaki, A., et al.: "Photorespiration protect C3 plants from photooxidation"Nature. 384. 557-560 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyama, T., et al.: "Cytokinin induces a rapid decrease in the levels of mRNAs for catalase, 3-hydroxy-3-methylglutaryl CoA reductase, lectin and other unidentified proteins in etiolated cotyledons of cucumber"Plant Cell Physiol.. 36. 95-114 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka, O., et al.: "Photoperiodic flowering of Cuscuta japonica cultured in vitro"Mem. Konan Univ. Sci. Ser.. 42. 143-151 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Teramoto, H., et al.: "Isolation and characterization of cDNAs for cytokinin-repressed genes"Plant Growth Regulation. 18. 59-70 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeba, G., et al.: "Photorespiration is an essential mechanism for the protection of C3 plants from photooxidation In Stress Responses of Photosynthetic Organisms (eds. K. Satoh and N. Murata)"Elsevir Amsterdam. 15-36 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 木崎暁子、竹葉 剛: "植物の陸上化と光呼吸" 遺伝. 51・7. 9-11 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Teramoto,H., Takeba,G.: "Noncoding RNA for CR20,a cytokinin-repressed gene of cucumber" Plant Mol.Biol.32. 797-808 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 竹葉 剛: "省施肥・ストレス耐性植物の育成" バイオサイエンス・インダストリー. 55. 35-36 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 竹葉 剛: "光呼吸についての2つの誤解" 光合成研究会会報. 21. 9-11 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 竹葉 剛: "光呼吸を抑制すると光呼吸が増加するか" 光合成研究会会報. 23. 13-18 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kozaki,A., Takeba,G.: "Photorespiration protect C3 plants from photooxidation." Nature. 557. 557-560 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeba,G.: "Stress Responses of Photosynthesis Organisms : Molecular Mechanisms and Molecular Regulations (eds.Satoh K.and Murata,N.)" Springer Verlag,Heidelberg, 21 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kozaki,A. and Takeba,G.: "Photorespiration protect C3 plants from photooxidation." Nature. 384. 557-560 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Teramoto,H., Toyama,T., Takeba,G.: "Noncoding RNA for CR20, a cytokinin-repressed gene of cucumber." Plant Mol Biol. 32. 797-808 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toyama,T., Teramoto and Takeba,G.: "The level of mRNA for psaL, which encodes a subunit of photosystem I, is increased by cytokinin in darkness in etiolated cotyledons of cucumber." Plant & Cell Phyoiol. 37. 1038-1041 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Teramoto,H., Momotani,E., Takeba,G. and Tsuji,H.: "Isolation and characterization of cDNAs for cytokinin-repressed genes." Plant Growth Regulation. 18. 59-70 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toyama,T., Teramoto,H., Takeba,G. and Tsuji,H.: "Cytokinin induces a rapid decrease in the levels of mRNAs for catalase, 3-hydroxy-3-methylglutaryl CoA reductase, lectin and other unidentified proteins in etiolated cotyledons of cucumber." Plant Cell Phyoil. 36. 95-114 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Teramoto,H., Toyama,T., Takeba,G. and Tsuji,H.: "Changes in expression of two cytokinin-repressed genes, CR9 and CR20, in relation to aging, greening and wounding in cucumber." Plauta. 196. 387-395 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takeba,G.: "Photorespiration is an essential mechanism for the protection of C3 plants from photooxidation. In Stress Responses of Photosynthesis Organisms : Molecular Mechanisms and Molecular Regulations (eds. Satoh K. and Murata,N.) Heidelberg" Springer Verlag, (1997(in press))

    • Related Report
      1996 Annual Research Report
  • [Publications] Kozaki,A.,Takeba,G.: "Photorespiration ia an essential mechanism to protect plants from photooxidation." Science. (in submit.). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takeba,G.,Kozaki,A.: "Quantitative estimation of the photochemical energy dissipated by photorespiration in tomato leaves." Plant physiol.(in submit.). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toyama,T.,Teramoto,H.,Takeba G.,Tsuji,H.: "Cytokinin induces a rapid decrease in the levels of mRNAs for catalase,3-hydroxy-3-methylglutaryl CoA reductase,lectin and other unidentified proteins in etiolated cotyledomns of cucumber." Plant Cell Physiol.36. 95-114 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Teramoto,H.,Toyama,T.,Takeba,G.,Tsuji,H.: "Changes in expression of two cytokinin-repressed genes,CR9 and CR20,in relation to aging,greening and wounding in cucumber." Planta. 196. 387-395 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Teramoto,H.,E.Momotani,G.Takeba,H.Tsuji: "Cloning of a cDNA for cyto-kinin-repressed gene in excised cucumber cotyledons." Planta. 193. 573-579 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takeba.G.: "Phoroprotective role of photorespiration." In The 34th NIBB conference″Responses of the photosynthetic apparatus to environmental light conditions.″. 130-132

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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