| Project/Area Number |
07556131
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
生物資源科学
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| Research Institution | Kinki University |
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Principal Investigator |
IRITANI Akira Kinki University, Faculty of BOST,Professor., 生物理工学部, 教授 (80026385)
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| Co-Investigator(Kenkyū-buntansha) |
MIYASHITA Minoru Osaka Tennoji Zoological Garden, Sec.of Breeding, A section chief, 飼育課, 係長
TAKENOSHITA Yoji Keari Co.Ltd., Reprod.Center, Director., 生産本部, 本部長
HOSOI Yoshihiko Kinki University, Faculty of BOST,Associate Professor., 生物理工学部, 助教授 (70192739)
IWAI Tamotsu Kinki University, Faculty of BOST,Professor, 生物理工学部, 教授 (60026490)
竹乃下 洋司 (株)ケアリー, 生産本部, 本部長
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1996: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | microfertilization / endangered species / embryo production / 顕微受精 / 死滅精子 / 凍結保存胚 / 胚発生 / 保護生物学 |
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| Research Abstract |
Advanced human IVF techniques are excellent methods of artificial breeding in animals with uncontrolled reproductive systems and also helpful for many endangered species. In our project, mainly ICSI of spermatozoa recovered from dead male animals in the zoo was done. In ICSI,the shape of sperm is an important factor for success rate in many species. In the design of a simple ICSI method for endangered species with sperm with different morphologies, it is beneficial for handling of gemetes to separate sperm head and tail. Therefore, we examined whether spermatozoa must be structurally intact before microsurgical injection into oocytes for normal fertilization using rabbit and cattle as models of wild life. Results obtained in rabbits indicated that 65% of injected oocytes with sperm head were normally fertilized, half were able to cleave, and the cleaved embryos were capable to development after transfer to recipient does. Despite confirmation of the pregnant at 14 days after transfer, no young was born. In the bovine systems, half of the successfully injected oocytes were normally fertilized and I 1% of eggs were cleaved. These results indicated that spermatozoa do not need to be structurally intact for intracytoplasmic injection and the sperm tail is not essential for normal development, at least to the cleavage stage in the rabbit. And also, we try to establish in vitro fertilization, ICSI (intracytoplasmic sperm injection) and embryo transfer of Japanese monkey system. These data indicate the usefulness as a method of artificial indoor breeding and also helpfulness for many endangered primates.
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