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Development of a genetic system to introduce alien useful genes into common wheat and to determine their loci

Research Project

Project/Area Number 07556132
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Breeding science
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

ENDO Takashi  Kyoto University, Graduate School of Agriculture, Professor, 農学研究科, 教授 (60068830)

Co-Investigator(Kenkyū-buntansha) MIYASHITA Naohiko  Kyoto University, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (20212243)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsChromosomal rearrangement / rye / common wheat / C-band / PCR / secalin / in situ hybridization
Research Abstract

Recently, Endo (1999) found that certain genes or chromosomes derived from wild species related to wheat induce chromosomal structural mutations in common wheat with high frequencies, and established a genetic system inducing chromosomal rearrangements. The present study aimed at generating deletion and translocation lines of a rye chromosome, which would be useful for the genetics and breeding of wheat, by applying the genetic system. After the two-year research we have achieved 1) the production of wheat lines with rye chromosome 1R in which chromosomal mutations occur and 2) the establishment of detection methods of structural changes involving chromosome 1R.As to 1), first, we crossed a wheat cultivar with a substitution of chromosome 1R for wheat chromosome 1B to an addition line of wheat with 3C chromosome of Aegilops triuncialis, and selected derivatives with a chromosome constitution, 2n=43 (20"+1""1R+1'3C) : Chromosome mutations are expected to occur in the progeny. Further, we selected derivative plants with 2n=44 (20"+1"1R+1"3C). Crosses between 2n=44 plants and the 1R/1B substitution cultivar would yield hybrids (20"+1"1R+1'3C) that would produce progeny carrying chromosomal mutations, involving those in chromosome 1R.Using these wheat lines, we will be able to conduct the screening of 1R structural changes in a large scale. As to 2), we demonstrated that C-banding is an effective technique to detect 1R terminal deletions, and that in situ hybridization can identify the rye segments involved in translocation with wheat chromosomes. Further, we established a PCR-based method to detect a gene for secalin, a seed storage protein, which is located on the satellite of chromosome 1R.By using these cytogenetical techniques we expect to be able to identify effectively structural changes involving chromosome 1R.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Endo, T. R.: "The deletion stocks of common wheat" The Journal of Heredity. 87. 295-307 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gill, K. S.: "Identification and high-density mapping of gene-rich regions in chromosome group 5 of wheat" Genetics. 143. 1001-1012 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamori, M.: "Variation of starch granule proteins and chromosome mapping of their coding genes in common wheat" Theoretical and Applied Genetics. 93. 275-281 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Endo, T. R.: "Allocation of a gametocidal chromosome of Aegilops cylindrica to wheat homoeologous group 2" Genes & Genetic Systems. 71. 243-246 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Endo, T. R.: "Production and utilization of deletion stocks in common wheat" Gamma Field Symposia. 34. 65-75 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Endo, T.R.: "The deletion stocks of common wheat" The Journal of Heredity.87. 295-307 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gill, K.S.: "Identification and high-density mapping of gene-rich regions in chromosome group 5 of wheat" Genetics.143. 1001-1012 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamori, M.: "Variation of starch granule proteins and chromosome mapping of their coding genes in common wheat" Theoretical and Applied Genetics.93. 275-281 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Endo, T.R.: "Allocation of a gametocidal chromosome of Aegilops cylindrica to wheat homoeologous group 2" Genes & Genetic Systems.71. 243-246 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Endo, T.R.: "Production and utilization of deletion stocks in common wheat" Gamma Field Symposia.34. 65-75 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Endo,T.R: "The deletion stocks of common wheat" The Journal of Heredity. 87. 295-307 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Gill,K.S.: "Identification and high-density mapping of gene-rich regions in chromosome group of wheat" Genetics. 143. 1001-1012 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamamori,M.: "Variation of starch granule proteins and chromosome mapping of their coding in common wehat" Theoretical and Applied Genetics. 93. 275-281 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Endo,T.R..: "Allocation of a gametocidal chromosome of Aegilops cylindrica to wheat homoeologous group 2." Genes & Genetic Systems. 71. 243-246 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Endo, TR: "The deletion stocks of common wheat" The Journal of Heredity. 87(in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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