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Breeding a hard, winter type durum wheat by the gene shuttling method

Research Project

Project/Area Number 07556134
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Breeding science
Research InstitutionFukui Prefectural University

Principal Investigator

TSUNEWAKI Koichiro  Fukui Pref.Univ., Dept.Bioscience, Professor, 生物資源学部, 教授 (20026438)

Co-Investigator(Kenkyū-buntansha) MURAI Takao  Hokuren Agr.Res.Inst., Sect.Plant Engeen., Researcher, 植物工学科, 研究員
IKEGUCHI Shoujiro  Hokuren Agr.Res.Inst., Sect.Plant Engineer., Researcher, 植物工学科, 研究員
MATSUOKA Yoshihiro  Fukui Pref.Univ., Dept.Bioscience, Lecturer, 生物資源学部, 助手 (80264688)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥4,900,000 (Direct Cost: ¥4,900,000)
KeywordsGene shuttling / Chromosome Engineering / Durum wheat / Bread wheat / Baking quality / Semidwarfism / Winter habit / 矮性 / 耐寒性
Research Abstract

Common wheat (genome, AABBDD) carries agronomically important genes, such as Glu-D1d gene (on chromosome 1D) for high baking quality, Rht2 gene (on 4D) for semidwarfism, and vrn3 (on 5D) for strong winter growth habit, which are not found in durum wheat (AABB). The present investigation aimed to transfer those genes to durum wheat using the D-genome chromosome substitution lines of a durum wheat.
(1) Transfer of the Glu-D1d gene : Langdon's 1D (1A) and 1D (1B) substitution lines were crossed to two bread wheat cultivars, Max and Stoa, which carry a Glu-D1d allele for the best glutenin fractions. Several F_2 plants having 2n=28 and Glu-D1d allele in the homozygous condition were successfully selected. Grains set by self-pollination were harvested and flour characteristics were tested. Two doses of the Glu-D1d allele did not change protein content of flour but its mixing tolerance clearly was increased, and loaf volume and texture were improved, comparing to the Langdon's flour.
(2) Transf … More er of the Rht genes : Japanese cultivar, Norin 10, that carries two semidwarf genes, Rht1 and Rht2, was crossed to Langdon and in the F_2 generation selection was made on chromosome number and plant height. An F_3 line was obtained that carries the Rht1 and vrn1 genes both in homozygous condition. In another experiment, crosses were made between Norin 10 and Langdon's 4D (4A) and 4D (4B) substitution lines and in the F_2 generation selection was made on chromosome number and plant height. Several short culm plants having nearly 2n=28 were obtained, which are assumed to be the Rht2 homozygote in the durum background.
(3) Transfer of the vrn genes : To produce a durum line having both the vrn1 and vrn3 genes, Langdon's 5D (5B) substitution line was crossed to two Japanese winter cultivars of common wheat, and selection was made on chromosome number in the F_2 generation, and on growth habit in the F_3 generation. Several F_3 plants having strong winter growth habit and nearly 2n=28 chromosomes were obtained, which are assumed to be the homozygote of both vrn genes. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Tsunewaki, K.& S.Matsuda: "Shuttling D-genome genes between common wheat and emmer wheat using the D-genome chromosome addition and substitution lines of emmer wheat" Proceedings of the 8th Internationa Wheat Genetics Symposium,Beijing. 1. 169-173 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 常脇 恒一郎・村井 政彦・斎藤 環: "矮性遺伝子Rht10の6倍性と4倍性コムギ間でのシャトリング" 育種学雑誌. 46・別冊1. 93 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 常脇 恒一郎・池口 正二郎・村井 達夫: "遺伝子シャトリング法による硬質マカロニコムギの育成(予報)" 育種学雑誌. 47・別冊1. 245 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 常脇 恒一郎・池口 正二郎・村井 達夫・松岡 由浩: "Dゲノム染色体置換マカロニコムギのGlu-D1d遺伝子ホモ系統の育成" 育種学雑誌. 47・別冊2. 368 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuoka, Y.& K.Tsunewaki: "Wheat retrotransposon families identified by reverse transcriptase domain analysis" Molecular Biology and Evolution. 13・10. 1384-1392 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsunewaki, K.and Matsuda, S.: "Shutting D-genome genes between common wheat and emmer wheat using the D-genome chromosome addition and substitution lines of emmer wheat" Proc.8th Int.Wheat Genet.Symp.Beijing. 1. 169-173 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsunewaki, K., Murai, M., and Saito, T.: "Shuttling the dwarf gene, Rht 10, between 6x and 4x wheat" Breed.Sci.46(Suppl.1). 93 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsunewaki, K., Ikeguchi, S.and Murai, T.: "Breeding hard durum wheat by the gene shuttling method (Preliminary report)" Breed.Sci.47(Suppl.1). 245 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsunewaki, K., Ikeguchi, S., Murai, T.and Matsuoka, Y.: "Breeding the Glu-D1d homozygotes in the D-genome chromosome substition lines of durum wheat" Breed.Sci.47(Suppl.2). 368 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuoka, Y.and Tsunewaki, K.: "Wheat retrotransposon families identified by reverse transcriptase domain analysis" Mol.Biol.Evol.13(10). 1384-1392 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 常脇恒一郎・池口正二郎・村井達夫: "遺伝子シャトリング法による硬質マカロニコムギの育成(予報)" 育種学雑誌. 47・別冊1. 245 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 常脇恒一郎・池口正二郎・村井達夫・松岡由浩: "Dゲノム染色体置換マカロニコムギのGlu-Dld遺伝子ホモ系統の育成" 育種学雑誌. 47・別冊2. 368 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 常脇恒一郎・村井政彦・斉藤環: "矮性遺伝子Rth10の6倍性と4倍性コムギ間のシャトリング" 育種学雑誌. 46・別冊1. 93- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsunewaki,K.and S.Matsuda: "Shuttling D-genome genes between common wheat and emmer wheat using the D-genome chromosome addition and substitution lines of duram wheat" Proceedings of the 8th International wheat Genetics Symposium. 1. 169-173 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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