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Breeding techniques for the content control of physiologically active substances in seed crops

Research Project

Project/Area Number 07556135
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Breeding science
Research InstitutionKyoto Prefectural University

Principal Investigator

TANAKA Kunisuke  Kyoto Prefectural University, Faculty of Agriculture, Professor, 農学部, 教授 (90027194)

Co-Investigator(Kenkyū-buntansha) OGIHARA Yasunari  Yokohama University, Kifara Institute for Biological Research, Associate Professor, 木原生物学研究所, 助教授 (40185533)
SATOH Hikaru  Kyushu University, Faculty of Agriculture, Institute of Genetic Resources, Professor, 農学部附属遺伝子資源研究センター, 教授 (70128031)
OHTSUBO Ken-ichi  National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Cereal Properties Laboratory, Chief, 食品総合研究所, 穀類特性研究室長
TAKANO Tetuo  University of Tokyo, Asian Natural Environmental Science Center, Associate Professor, アジア生物資源環境研究センター, 助教授 (30183057)
OHSAWA Ryo  Hokuriku National Agricultural experiment Station, Department of Lowland Farming, Senior Researcher, 北陸農業試験所, 主任研究官
柴田 大輔  三井業際バイオ研究所, 主任研究員
喜多村 啓介  農水省, 農業研究センター, 作物開発室長
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1996: ¥3,600,000 (Direct Cost: ¥3,600,000)
Keywordsseed / allergen / amylase inhibitors / trypsin inhibitors / lipoxygenase / subtilicin inhibitor / rutin / wheat / 種子作物 / イネアレルゲン / イネズブチリシンインヒビター / α-アミラーゼインヒビター / イネリポキシナーゼ / ソバルチン / イネトリプシンインヒビター
Research Abstract

Following results have been obtained through activities of the groups listed below, Activities have been focused on the establishment of breeding techniques for the content control of physiologically active substances in seed crops.
・ Biochemical analysis group:
1) The analytical accuracy of the isofocusing eletrophoresis for rice allergenic substances has dramatically improved.
2) The detection sensitivity for rice subtilicin inhibitor (RASI) and rice trypsin inhibitor (RATI) was improved greatly by using antigen-antibody method.
3) The detection technique to detect the existence of the photoperiod-sensitivity cytoplasmic male sterility factor was improved.
・ Genetic engineering group:
4) The membrane recognizing ability of rice storage protein signal sequences was successfully changed introducing mutations in the sequences.
5) Mutations were introduced into the promoter region of a soybean lipoxygenase gene and the promoter activity was changed.
6) The direction was fixed to remove allergenic substances from soybean by the isolation of two novelgenes.
・ screening Group:
7) By use of a new analytical method a line that increased the rutin contents up to 30% was found.
8) New soybean varieties were screened they were expected to have good taste.
9) Genetic variations of barley amylase inhibitor activities were analyzed using molecular markers.
Results described above will be useful for the screening of varieties of physiologically active substances in the seed crops.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 大坪研一: "RAPD法を用いた国内産精米の品種判別技術"日本食品化学工学会誌. 44(5). 386-390 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shiken Sha: "Structure of the rice glutaredoxin(thioltransferase)gene"Gene. 188. 23-28 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasunari Ogihara: "Alloplasic wheats with Aegilops crassa cytoplasm which express photopeiod-sensitive homeotic transformations of anthers,show alterations in mitochondorial DNA structure and transcription"Mol.Gen.Genet. 255. 45-53 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Koji Murai: "Wheat MADS box genes,a multigene family dispersed throughout the genome"Genes Genet.Syst. 72. 317-321 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Kumamaru: "High-lysine mutants of rice, Oryza sativa L"Plant Breeding. 116. 245-249 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuhiro Yanagisawa: "Change in Free Amino Acid and Kjeldahl N Concentrations in Seeds from Vegetable-Type and Grain-Type Soybean Cultivars during the Cropping Season"J.of Agric.and Food Chem. 45(5). 1720-1724 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasunari ogihara: "Alloplasmic wheats with Aegilops crassa cytoplasm which express photoperiod-senstive homeotic teansformations of anthers, show alterations in mitochondrial DNA structure and transcription"Mol. Gen. Genet.. 255. 45-53 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Koji Murai: "Wheat MADS box genes, a multigene family dispersed throughout the genome"Genes Genet. Syst.. 72. 317-321 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T. Kumamaru: "High-Lysine mutants of rice, Oryza sativa L."Plant Breeding. 116. 245-249 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuhiro Yanagisawa: "Change in Free Amino Acid and Kjeldahl N Concentrations in Seeds from Vegetable-Type and Grain-Type Soybean Cultivars during the Cropping Season"J. of Agric. And Food Chem.. 45(5). 1720-1724 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大坪 研一: "RAPD法を用いた国内産精米の品種判別技術" 日本食品化学工学会誌. 44(5). 386-390 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shiken Sha: "Structure of the rice glutaredoxin (thioltransferase) gene" Gene. 188. 23-28 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasunari Ogihara: "Alloplasmic wheats with Aegilops crassa cytoplasm which express photoperiod-senstive homeotic transformations of anthers, show alterations in mitochondrial DNA structure and transcription" Mol.Gen.Genet. 255. 45-53 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Koji Murai: "Wheat MADS box genes,a multigene family dispersed throughout the genome" Genes Genet.Syst.72. 317-321 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Kumamaru: "High-lysine mutants of rice,Oryza sativa L." Plant Breeding. 116. 245-249 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuhiro Yanagisawa: "Change in Free Amino Acid and Kjeldahl N Concentrations in Seeds from Vegetable-Type and Grain-Type Soybean Cultivars during the Cropping Season" J.of Agric.and Food Chem.45(5). 1720-1724 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fumio Sugawara: "Insecticidal Peptide from Mungbean : A Resistant Factor against Infestation with Azuki Bean Weevil" J. Agric. Food Chem.44. 3360-3364 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高野 哲夫: "大豆の形質転換技術の効率化とそれを利用したアレルゲンたん白質の低減化" 大豆たん白質研究会誌. 17. 14-18 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 相澤 弥生: "コムギにおける花器官形成調節遺伝子の機能 解析 III. RT-PCRによる花器官特異的発現遺伝子の検索" 育種学雑誌. 45(別冊). 1-29 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大坪 研一: "米などイネ科穀物の成分・特性の評価手法及び適正利用技術に関する研究" 日本食品科学工学会誌. 42(12). 1046-1053 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Daisuke Shibata: "Plant Lipoxygenase Genes in Lipoxygenase and Lipoxygenase Pathway Enzymes" AOCS Press, 39-55 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shiken Sha: "Cloning and Sequencing of a Rice Gene Encoding the 13-kDa Prolamin Polypeptide" Biosci.Biotech.Biochem.60(2). 335-337 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Zhenwei Zheng: "The Bean Seed Storage Protein β-Phaseolin Is Synthesized,Processed,and Accumulated in the Vacuolar Type-II Protein Bodies of Transgenic Rice Endosperm" Plant Physiol.109. 777-786 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 増村威宏: "食品工業における科学技術の進歩(VI)米蛋白質の化学-イネ種子蛋白質の生合成と集積機構-" 光琳, 20 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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