| Project/Area Number |
07557004
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
General physiology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
ARAISO Tsunehisa Hokkaido Univ., Center for Advanced Science and Technology, Assoc.Prof., 先端科学技術共同研究センター, 助教授 (30151145)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | microscopic fluorometry / time-resolved fluorescence depolarization / plasma membrane / single molecular layr / molecular motion / molecular orientation / pulsed laser / LB-membrane / 時間分解・蛍光偏光解消 |
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| Research Abstract |
Time-resolved fluorescence depolarization method is a powerful tool to measure molecular motion of biological membranes. Usually this type of the measurement has been applied to membrane or cell suspension, and so the results were averaged values of the ensemble of molecules in the membranes or cells. However physiologically important changes of the molecular motions in the membranes occur at local areas. In this work, we use a microscope to develop the fluorescence depolarization method to detect the molecular motion at a local area with micrometer resolution. A pulsed Ar-dye laser was introduced to the sample membrane located on a microscope stage for excitation. The incident angle was variable. This function will be useful to detect the orientation angle of the molecules in membrane. Decay of the polarized fluorescent light passed through the objective lens was detected by a single photon counting technique. With this system, we could measure the anisotropic fluorescence decay from a single molecular layr of stearic acid containing 1 mol% of DPH-propionate in a 10 square micrometer area. This suggests that this system can be applied to measure molecular motion in a local area of cell membrane.
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