| Project/Area Number |
07557006
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
General physiology
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| Research Institution | Tokai University School of Medicine |
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Principal Investigator |
NAKAZAWA Hiroe Tokai University, Department of physiology, Professor, 医学部, 教授 (20110885)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIDA Hideyuki Tokai University, Department of physiology, Assistant, 医学部, 講師 (20222424)
ICHIMORI Kohji Tokai University, Department of physiology, Assistant, 医学部, 講師 (60184636)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1996: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | superoxide / nitric oxide / peroxynitrite / hemoglobin / NO-electrode / Cytotoxicity / Cylotoxicity |
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| Research Abstract |
An NO-selective electrode was developed as an easily applicable tool for a real-time nitric oxide (NO) measurement. The working electrode (0.2 mm diam) was made from Pt/Ir alloy coated with a three-layred membrane. The counterelectrode was made from a carbon fiber. When a stable NO donor, S-nitroso-N-acetyl-dl-penicillamine, was applied, the electrode current increased in a dose-dependent fashion. The current and calculated NO concentration showed a linear relationship in the range from 0.2 nM (S/N=1) to 1 muM of NO.The response of the electrode was 1.14(]SY.+-。[)0.09s. The effects of temperature, pH,and chemicals other than NO on the electrode current were also evaluated. Electrodes which were placed in the luminal side of rat aortic rings exhibited 30 pA of current due to NO generation induced by the addition of 10<@D1-6@>D1M of acetylcholine. The current was eliminated in the presence of 50 muM N<@D1G@>D1-monomethyl-L-arginine, an inhibitor of NO synthase. Thus, this NO-selective electrode is applicable to real-time NO assay in biological systems.
NO+2H_2O*NO^-_+4H^++3e^-.
A positive voltage of approximately 0.8 V was applied t discriminate against the current from possible contaminant
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