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Humanized Animal Models for Pharmacological Studies

Research Project

Project/Area Number 07557012
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field General pharmacology
Research InstitutionThe University of Tokyo (1996-1997)
Kyushu University (1995)

Principal Investigator

MOTOYA Katsuki  The Institute of Medical Science The University of Tokyo, Professor, 医科学研究所, 教授 (20051732)

Co-Investigator(Kenkyū-buntansha) YOKOYAMA Minesuke  Mitsubishi Kasei Institute of Life Sciences, Chief, 生殖研究室, 室長
NAKAO Kazuki  The Institute of Medical Science The University of Tokyo, Research Associate, 医科学研究所, 寄付研究部員教員 (20217657)
NAKAMURA Kenji  The Institute of Medical Science The University of Tokyo, Research Associate, 医科学研究所, 助手 (90253533)
AIBA Atsu  The Institute of Medical Science The University of Tokyo, Associate Professor, 医科学研究所, 助教授 (20271116)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 1997: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1996: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1995: ¥8,200,000 (Direct Cost: ¥8,200,000)
Keywordsanimal models / pharmacology / serotonin receptor / serotonin / gene targeting / gene replacement / ES cell / humanized mouse / ジーンターゲッテイング
Research Abstract

An experiment using animal models is one of the most important projects for pharmacological studies. The development of novel drugs, in which the action should first be proved theoretically, may be possible by performing experiments using animal models. However, there are almost always. some differences between humans and animals Although serotonin acts through serotonin receptors, the mouse serotonin receptor 1B does not respond to either the agonists or antagonists developed for human serotonin receptor 1B.This difference was overcome by changing the 355th amino acid residue, threonin, of the human protein to aspargine, which is the equivalent residue of the mouse protein This means that even the single amino acid difference between humans and animals thus made it impossible to extrapolate the results from animal experiments to human beings.
We developed novel methods to replace the target gene with the desired sequences, such as a point mutation, small deletion, or human homologue, without any marker genes. In this study, we applied this method to the serotonin receptor 1B gene. We thereby succeeded in making the ES cell lines which carry the humanized serotonin 1B gene in place of the mouse gene .Chimera mice were also produced and we are now looking forward to obtaining the offspring of this germ line transmission.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (107 results)

All Other

All Publications (107 results)

  • [Publications] Koera, K., et al.: "K-Ras is essential for the development of the mouse embryo." Oncogene. 15. 1151-1159 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mimura, J., et al.: "Loss of teratogenic response to 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)in mice lacking the Ah(dioxin)receptor." Gene to Cells. 2. 645-654 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Araki, E., et al.: "Targeted disruption of exon 52 in the mouse dystrophin gene induced muscle degeneration similar to that observedin Duchenne muscular dystrophy." Biochem Biophys Res Commun. 238. 492-497 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakao, K., et al.: "Simple and efficient vitrification procedure for cryopreservation of mouse embryos." Exp Anim. 46. 231-234 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fukui, Y., et al.: "Positive and negative CD4+ thymocyte selection by a single MHC class II/peptide ligand affected by its expression level in the thymus." Immunity. 6. 401-410 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohmi, K., et al.: "A novel aortic smooth muscle cell line obtained from p53 knock out mice expresses several differentiation characteristics." Biochem Biophys Res Commun. 238. 154-158 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kameya, S., et al.: "Dp260 disrupted mice revealed prolonged implicit time of the β-wave in ERG and loss of accumulation of b-dystroglycan in the outer plexiform layer ofthe retina." Hum Mol Genet. 6. 2195-2203 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shibata, H., et al.: "An oocyte-specific methylation imprint center in the mouse U2afbp-rs/U2afl-rsl gene marks the establishment of allele-specific methylation during preimplantation development." Genomics. 44. 171-178 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shinohara, N., et al.: "Prevention of autoantibody production in lpr/lpr mice by transgenic expression of fas on B cells." Ann New York Acad Sci. 815. 189-491 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kadotani, H., et al.: "Motor discoordination results from combined gene disruption of the NMDA receptor NR2A and NR2C subunits,but not from single disruption of the NR2A or NR2C subunits." J Neurosci. 16. 7859-7867 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoi, M., et al.: "Impariment of hippocampal mossy fiber LTD in mice lacking mGluR2." Science. 273. 645-647 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shibata, H., et al.: "Inactive allele-specific metheylation and chromatin structure of the imprinted gene U2afl-rsl on mouse chromosome 11." Genomics. 35. 248-252 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitamoto, T., et al.: "Humanized prion protein knock-inby Cre-induced site-specific recombination in the mouse." Biochem Biophys Res Commun. 222. 742-747 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kato, T., et al.: "Inactivation of hepatitis C virus cDNA transgene by hypermethylation intransgenic mice." Arch Virol. 141. 951-958 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Inoue, R., et al.: "Rare activation of the human c-Ha-ras transgene of mice in hemangioendothelial sarcomas and liver tumors inducedby Glu-P-1." Jpn J Cancer Res. 87. 583-588 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuzuki, T., et al.: "Targeting disruption of the DNA repair methyltransferase gene renders mice hypersensitive to alkylating agent." Carcinogenesis. 17. 1215-1220 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohbo, K., et al.: "Modulation of hematopoiesis in mice with a truncated mutant of the interleukin-2 receptor γ chain." Blood. 87. 956-967 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Li, Y., et al.: "Establishiment of transgenic mice carrying human fetus-specific CYP3A7." Arch Biochem Biophys. 329. 235-240 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Gondo, Y., et al.: "A novel positive detection system of in vivo mutations in rpsL(strA)transgenic mice." Mutation Res. 360. 1-14 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Norimura, T., et al.: "p53-dependent apoptosis suppresses radeation-induced teratogenesis." Nature Medicine. 2. 577-580 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamaguchi, H., et al.: "Dopamine D2 receptor plays a critical role in cell proliferation and proopiomelanocortin expression in the pituitary" Genes to Cells. 1. 253-268 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tokita, Y., et al.: "Characterization of excitatory amino acid neurotoxicity in N-methyl-D-aspartate receptor-deficient mouse cortical neuronal cells" Eur J Neurosci. 8. 69-78 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kajiwara, K., et al.: "Cloning and characterization of pentylenetetrazol-related cDNA,PTZ-17." Brain Res. 671. 170-174 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimizu-Nishikawa, K., et al.: "Cloning and expression of SEZ-6,a brain-specific and seizure-related cDNA." Mol Brain Res. 28. 201-210 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masu, M., et al.: "Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene." Cell. 80. 757-765 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimano, H., et al.: "Inhibition of diet-induced atheroma formation in transgenic mice expressing apolipoprotein E in the arterial wall." J Clin Invest. 95. 469-476 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Honda, H., et al.: "Increased tyrosine-phosphorylation of 55KDa proteins in β-actin/Tec transgenic mice." Biochem Biophys Res Commun. 206. 287-293 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamura, H., et al.: "Opaque eyes developed in transgenic mice with T-cell receptor δ gene." Invest Ophthalmol Vis Sci. 36. 467-477 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okazaki, Y., et al.: "Direct detection and isolation of RLGS spot DNA marker tightly linked toa specific trait using RLGS spot-bombing method." Proc Natl Acad Sci USA. 92. 5610-5614 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirotsune, S., et al.: "The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons." Nature Genet. 10. 77-83 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kamataki, T., et al.: "Expressioon of CYP3A7,a human fetus-specific cytochrome P450,in culturedcells and in the hepatocytes of p53-knockout mice." Toxicol Lett. 82/83. 879-882 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimoji, M., et al.: "Establishment of immoratal hepatocytes from a CYP3A7-transgenic/p53-knockout mouse." Biochem Biophys Res Commun. 217. 1001-1005 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuroiwa, Y., et al.: "Peg 3 imprinted gene on proximalchromosome 7 encodes for a zinc finger protein." Nature Genet. 12. 186-190 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohoka, Y., et al.: "In vitro differentiation and commitment of CD8^+ CD8^+ thymocytes to the CD4 lineage without TCR engagement." Int Immunol. 8. 297-306 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kaneko-Ishino, T., et al.: "Pegl/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization." Nature Genet. 11. 52-59 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoyama, M., et al.: "The creation of mouse models forhuman diseases associated with reproductive distrubances by in vitro fertilization and embryo transfer." Exp Anim. 44. 139-143 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoyama, M., et al.: "Conversion of the genetic background of transgenic mice by in vitro fertilization." Exp Anim. 44. 145-150 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Koera, K., et al.: "K-Ras is essential for the development of the mouse embryo." Oncogene. 15. 1151-1159 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mimura, J., et al.: "Loss of teratogenic response to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in mice lacking the Ah (dioxin) receptor." Gene to Cells. 2. 645-654 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Araki, E., et al.: "Targeted disruption of exon 52 in the mouse dystrophin gene induced muscle degeneration similar to that observed in Duchenne muscular dystrophy." Biochem Biophys Res Commun. 238. 492-497 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakao, K., et al.: "Simple and efficient vitrification procedure for cryopreservation of mouse embryos." Exp Anim. 46. 231-234 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fnkui, Y., et al.: "Positive and negative CD4+ thymocyte selection by a single MHC class II/peptide ligand affected by its expression level in the thymus." Immunity. 6. 401-410 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohmi, K., et al.: "A novel aortic smooth muscle cell line obtained from p53 knock out mice expresses several differentiation characteristics." Biochem Biophys Res Commun. 238. 154-158 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kameya, S., et al.: "Dp260 disrupted mice revealed prolonged implicit time of the b-wave in ERG and loss of accumulation of beta-dystroglycan in the outer plexiform layr of the retina." Hum Mol Genet. 6. 2195-2203 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shibata, H., et al.: "An oocyte-specific methylation imprint center in the mouse U2afbp-rs/U2afl-rslgene marks the establishment of allele-specific methylation during preimplantation development." Genomics. 44. 171-178 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shinohara, N., et al.: "Prevention of autoantibody production in lpr/lpr mice by transgenic expression of fas on B cells." Ann New York Acad Sci. 815. 189-491 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kadotani, H., et al.: "Motor discoordination results from combined gene disruption of the NMDA receptor NR2A and NR2C subunits, but not from single disruption of the NR2A or NR2C subunits." J Neurosci. 16. 7859-7867 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoi, M., et al.: "Impariment of hippocampal mossy fiber LTD in mice lacking mGluR2." Science. 273. 645-647 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shibata, H., et al.: "Inactive allele-specific metheylation and chromatin structure of the imprinted gene U2afl-rsl on mouse chromosome 11." Genomics. 35. 248-252 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitamoto, T., et al.: "Humanized prion protein knock-in by Cre-induced site-specific recombinationin the mouse." Biochem Biophys Res Commun. 222. 742-747 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kato, T., et al.: "Targeted disruption of the DNA repair methyltransferase gene renders mice hypersensitive to alkylating agent." Arch Virol. 141. 951-958 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Inoue, R., et al.: "Rare activation of the human c-Ha-ras transgene of mice in hemangioendothelial sarcomas and liver tumors induced by Glu-P-l." Jpn J Cancer Res. 87. 583-588 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuzuki, T., et al.: "Targeting disruption of the DNA repair methyltransferase gene renders mice hypersensitive to alkylating agent." Carcinogenesis. 17. 1215-1220 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohbo, K., et al.: "Modulation of hematopoiesis in mice with a truncated mutant of the interleukin-2 receptor gamma chain." Blood. 87. 956-967 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Li, Y., et al.: "Establishiment of transgenic mice carrying human fetus-specific CYP3A7." Arch Biochem Biophys. 329. 235-240 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Gondo, Y., et al.: "A novel positive detection system of in vivo mutations in rpsL (strA) transgenic mice." Mutation Res. 360. 1-14 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Norimura, T., et al.: "p53-dependent apoptosis suppresses radeation-induced teratogenesis." Nature Medicine. 2. 577-580 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamaguchi, H., et al.: "Dopamine D2 receptor plays a critical role in cell proliferation and proopiomelanocortin expression in the pituitary." Genes to Cells. 1. 253-268 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tokita, Y., et al.: "Characterization of excitatory amino acid neurotoxicity in N-methyl-D-aspartate receptor-deficient mouse cortical neuronal cells" Eur J Neurosci. 8. 69-78 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kajiwara, K., et al.: "Cloning and characterization of pentylenetetrazol-related cDNA,PTZ-17." Brain Res. 671. 170-174 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimizu-Nishikawa, K., et al.: "Cloning and expression of SEZ-6, a brain-specific and seizure-related cDNA." Mol Brain Res. 28. 201-210 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masu, M., et al.: "Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene." Cell. 80. 757-765 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimano, H., et al.: "Inhibition of diet-induced atheroma formation in transgenic mice expressing apolipoprotein E in the arterial wall." J Clin Invest. 95. 469-476 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Honda, H., et al.: "Increased tyrosine-phosphorylation of 55KDa proteins in beta-actin/Tec transgenic mice." Biochem Biophys Res Commun. 206. 287-293 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamura, H., et al.: "Opaque eyes developed in transgenic mice with T-cell receptor delta gene." Invest Ophthalmol Vis Sci. 36. 467-477 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okazaki, Y., et al.: "Direct detection and isolation of RLGS spot DNA marker tightly linked to a specific trait using RLGS spot-bombing method." Proc Natl Acad Sci USA. 92. 5610-5614 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirotsune, S., et al.: "The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons." Nature Genet. 10. 77-83 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kamataki, T., et al.: "Expressioon of CYP3A7, a human fetus-specific cytochrome P450, in cultured cells and in the hepatocytes of p53-knockout mice." Toxicol Lett. 82/83. 879-882 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimoji, M., et al.: "Establishment of immoratal hepatocytes from a CYP3A7-transgenic/p53 knockout mouse." Biochem Biophys Res Commun. 217. 1001-1005 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuroiwa, Y., et al.: "Peg 3 imprinted gene on proximal chromosome 7 encodes for a zinc finger protein." Nature Genet. 12. 186-190 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohoka, Y., et al.: "In vitro differentiation and commitment of CD8^+ CD8^+ thymocytes to the CD4 lineage without TCR engagement." Int Immunol. 8. 297-306 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kaneko-Ishino, T., et al.: "Pegl/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization." Nature Genet. 11. 52-59 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoyama, M., et al.: "The creation of mouse models for human diseases associated with reproductive-distrubances by in vitro fertilization and embryo transfer." Exp Anim. 44. 139-143 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoyama, M., et al.: "Conversion of the genetic background of transgenic mice by in vitro fertilization." Exp Anim. 44. 145-150 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Koera,K., et al.: "K-Ras is essential for the development of the mouse embryo." Oncogene. 15. 1151-1159 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mimura,J., et al.: "Loss of teratogenic response to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in mice lacking the Ah (dioxin) receptor." Gene to Cells. 2. 645-654 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Araki,E., et al.: "Targeted disruption of exon 52 in the mouse dystrophin gene induced muscle degeneration similar to that observed in Duchenne muscular dystrophy." Biochem Biophys Res Commun. 238. 492-497 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakao,K., et al.: "Simple and efficient vitrification procedure for cryopreservation of mouse embryos." Exp Anim. 46. 231-234 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fukui,Y., et al.: "Positive and negative CD4+thymocyte selection by a single MHC class II/peptide ligand affected by its expression level in the thymus." Immunity. 6. 401-410 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohmi,K., et al.: "A novel aortic smooth muscle cell line obtained from p53 knock out mice expresses several differentiation characteristics." Biochem Biophys Res Commun. 238. 154-158 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kameya,S., et al.: "Dp260 disrupted mice revealed prolonged implicit time of the b-wave in ERG and loss of accumulation of b-dystroglycan in the outer plexiform layer of the retina." Hum Mol Genet. 6. 2195-2203 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shibata,H., et al.: "An oocyte-specific methylation imprint center in the mouse U2afbp-rs/U2af1-rs1 gene marks the establishment of allele-specific methylation during preimplantation development." Genomics. 44. 171-178 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shinohara,N., et al.: "Prevention of autoantibody production in 1pr/1pr mice by transgenic expression of fas on B cells." Ann New York Acad Sci. 815. 189-491 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kadatani,H.,et al.: "Motor discoordination results from combined gene disruption of the NMDA receptor NR2A and NR2C subunits,but not from single disruption of the NR2A or NR2C subunits." J Neurosci. 16. 7859-7867 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yokoi,M.,et al.: "Impariment of hippocampal mossy fiber LTD in mice lacking mGluR2." Science. 273. 645-647 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kato,T.,et al.: "Inactivation of hepatitis C virus cDNA transgene by hypermethylation intransgenic mice." Arch Virol. 141. 951-958 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Inoue,R.,et al.: "Rare activation of the human c-Ha-ras transgene of mice in hemangioendothelial sarcomas and liver tumors induced by Glu-P-1." Jpn J Cancer Res. 87. 583-588 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohbo,K.,et al.: "Modulation of hematopoiesis in mice with a truncated mutant of the interleukin-2 receptor gamma chain." Blood. 87. 956-967 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Li,Y.,et al.: "Establishiment of transgenic mice carrying human fetus-specific CYP3A7." Arch Biochem Biophys. 329. 235-240 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Gondo,Y.,et al.: "A novel positive detection system of in vivo mutations in rpsL(strA) transgenic mice." Mutation Res. 360. 1-14 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Norimura,T.,et al.: "p53-dependent apoptosis suppresses redeation-induced teratogenesis." Nature Medicine. 2. 577-580 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi,T.,et al.: "Dopamine D2 receptor plays a critical role in cell proliferation and proopiomelanocortin expression in the pituitary." Genes to Cells. 1. 253-268 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tokita,Y.,et al.: "Characterization of excitatory amino acid neurotoxicity in N-methyl-D-aspartate receptor-deficient mouse cortical neuronal cells." Eur J Neurosci. 8. 69-78 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shibata,H.,et al.: "Dysfunction of the Orleans reeler gene arising from exon skipping due to transposition of a full-length copy of an active L1 sequence into the skipped exon." Hum Mol Genet. 5. 989-993 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kitamoto,T.,et al.: "Humanized prion protein knock-in by Cre-induced site-specific recombination in the mouse." Biochem Biophys Res Commun. 222. 742-747 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsuzuki,T.,et al.: "Targeted disruption of the Rad51 gene leads to lethality in embryonic mice." Proc Natl Acad Sci USA. 93. 6236-6240 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi,H.,et al.: "Dopamine D2 receptor plays a critical role in cell proliferation and proopiomelanocortin expression in the pituiary." Genes to Cells. in press.

    • Related Report
      1995 Annual Research Report
  • [Publications] Gondo,Y.,et al.: "A novel positive detection system of in vivo mutations in rpsL(strA)transgenic mice." Mutation Res. in press.

    • Related Report
      1995 Annual Research Report
  • [Publications] Masu,M.,et al.: "Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene." Cell. 80. 757-765 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shimano,H.,et al.: "Inhibition of diet-induced atheroma formation in transgenic mice expressing apolipoprotein E in the arterial wall." J Clin Invest. 95. 469-476 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Honda,H.,et al.: "Increased pyrosine-phosphorylation of 55KDa proteins in beta-actin/Tec transgenic mice." Biochem Biophys Res Commun. 206. 287-293 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tamura,H.,et al.: "Opaque eyes developed in transgenic mice with T-cell receptor δ gene." Invest Ophthalmol Vis Sci. 36. 467-477 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kano,M.,et al.: "Impaired synapse elimination during cerebellar development in PKCγ mutant mice." Cell. 83. 1223-1231 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hsia,A.Y.,et al.: "Evidence against a role for metabotropic glutamate receptors in mossy fiber LTP:the use of mutani mice and pharmacological antagonists." Neuropharmacol. 34. 1567-1572 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yokoyama,M.,et al.: "The creation of mouse models for human diseases associated with reproductive disturbances by in vitro fertilization and embryo transfer." Exp Anim. 44. 139-143 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yokoyama,M.,et al.: "Conversion of the genetic background of transgenic mice by in vitro fertilization." Exp Anim. 44. 145-150 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kaneko-Ishino,T.,et al.: "Pegl/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization." Nature Genet. 11. 52-59 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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