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Development of a New High Performance Confocal Laser Scanning Microscpe Enabling the Observation of Dynamic Changes of Subcellular Organelles.

Research Project

Project/Area Number 07557025
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Experimental pathology
Research InstitutionTokai University

Principal Investigator

WATANABE Keiichi  Tokai University School of Medicine Professor, 医学部, 教授 (00055865)

Co-Investigator(Kenkyū-buntansha) OSAMURA Yoshiyuki  Tokai University School of Medicine Professor, 医学部, 教授 (10100992)
WAKAKI Moriaki  Tokai University School of Engineering Professor, 工学部, 教授 (20100993)
守内 哲也  東海大学, 医学部, 助教授 (20174394)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥15,500,000 (Direct Cost: ¥15,500,000)
Fiscal Year 1996: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1995: ¥12,000,000 (Direct Cost: ¥12,000,000)
KeywordsConfocal Laser Scanning Microscope / Fine structures living cells / Very short wave length laser / CLSM for engineering / Resolution power in vertical and horizontal axises / 共焦点レーザー顕微鏡 / 細胞内小器官 / 光顕 / 電顕 / 空間分解能 / ヘリウム-カドニウムレーザーHe-Cd
Research Abstract

The application of the confocal laser scanning microscope (CLSM) in the filed of cell biology brought about outstanding progress in observing functional states of cells through the combination with various cytochemical techniques. The observation enabled not only to confirm the localizations of biologically active substances at almost ultrastructural levels but also catch up their 3 dimensional configurations. Recently, the CLSM application has been extended to observe living cells to observe the time dependent morphological and functional changes of the cells. We have to admit, however, that the considerable distance has still been left from the ultimate goal of those attempts. For instance, in the observations of intracellular localizations of biologically active substances, the clearities of the configurations of subcellular organelles are still far less than those of electron microscopy. This inferiority may largely be due to the evidence that the thickness of "optical (tomographic) sections" obtained in CLSM (more than 300nm) is still much bigger than that of electron microscopy (less than 100nm). In the present study, we intended to develop and construct a new CLSM which can manage such problems and would cope with the observation of very fine structures of living cells. For this purpose, we selected a CLSM (JDLM-6602) produced by JEOL Liosonic Co.Ltd.as a prototype which was specially designed to observe very fine surface structures of semiconductors by obtaining excellent resolution especially in vertical axis and by making "pin hole" much smaller. We installed extraneously the He-Cd laser radiation unit (the wave length as short as 325nm) to improve the resolution. We also applied a high performance photon counter to detect very weak signals from the specimens.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 海老塚昇、小林行泰、佐藤修二、若木守明: "新しい型の2次元マルチチャンネルフーリエ分光計の開発と実用化" 分光研究. 44(3). 131-140 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 伊藤丈夫、安田政実、伊東良子、平園賢一、長村義之、渡辺慶一: "非蛍光プローブCLSMの応用-ホルモン産生上皮性卵巣悪性腫瘍の1例-" レーザ顕微鏡研究会. 15. 51-58 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noboru Ebizuka,Moriaki Wakaki,Yykiyasu Kobayashi,Syuji Sato: "Development of a Multichannel Fourier Transform Spectrometer." Applied Optics. 34(3). 7899-7906 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Wakaki,Y.Komachi,H.Machida,H.Kobayashi: "Fiber-optic Polarizer Using Birefringent Crystal as a Cladding." Applied Optics. 35(15). 2591-2594 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 伊藤丈夫、山王なほ子、篠生環、松野彰、渡辺慶一、長村義之: "In Situ Hybridization(ISH)へ非蛍光プローブCLSMの応用-ヒト下垂体GH産生腺腫の捺印標本におけるGH mRNAの解析-" レーザ顕微鏡研究会. 17. 89-96 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 伊東丈夫、渡辺慶一: "新しい光学顕微鏡第2巻共焦点レーザー顕微鏡;非蛍光プローブ" 学際企画(東京), 199(9) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 渡辺慶一: "光量子科学の誕生と未来像-新たな科学技術人系のためのハンドブック(未来医療におけるレーザーの役割)" 科学技術広報財団(科学技術庁原子力局.監修), 488(6) (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noboru Ebizuka, Moriaki Wakai, Yukiyasu Kobayashi, Syuji Sato: "Development of a Multichannel Fourier Transform Spectrometer." Applied Optics. 34 (3). 7899-7906 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Wakaki, Y.Komachi, H.Machida, H.Kobayashi: "Fiber-optic Polarizer Using Birefringent Crystal as a Cladding." Applied Optics. 35 (15). 2591-2594 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jobu Itoh, Keiichi Watanabe: New Light Microscopes : Book II,Confocal Laser Scanning Microscopes ; Application of non-fluorescent probes". Gakusaikikaku, Tokyo, 1995

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Keiichi Watanabe: Photon Science : Its outbreak and future -Handbook for the New Science and Technology, "The Role of Laser in the Future Medicine" -. Foundation for Science and Technology Informations, October, 1996

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Wakaki,Y.Komachi,H.Machida,H.Kobayashi: "Fiber-optic Polarizer Using Birefringent Crystal as a Cladding." Applied Optics.

    • Related Report
      1996 Annual Research Report
  • [Publications] 伊東丈夫、山王なほ子、篠生環、松野彰、渡辺慶一、長村義之: "In Situ Hybridization (ISH)へ非蛍光プローブCLSMの応用-ヒト下垂体GH産生腺腫の捺印標本におけるGH mRNAの解析-" レーザ顕微鏡研究会.

    • Related Report
      1996 Annual Research Report
  • [Publications] 渡辺慶一: "光量子科学の誕生と未来像-新たな科学技術人系のためのハンドブック(未来医療におけるレーザーの役割)" 科学技術広報財団(科学技術庁原子力局.監修),

    • Related Report
      1996 Annual Research Report
  • [Publications] Takekoshi, S. Watanabe, K. et al: "Activation of protein Kinase C by oxidized diacylglycerols" Biochem. Biophys. Res. Commun.217. 654-660 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Murakoshi, M. Watanabe, K. et al: "Immunolocalization of glutathione-reductase (GSSG-RD) in the rat adrenal cortex" J. Toxicol Pathol.8. 51-57 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tanaka, M. Watanabe, K. et al: "Immunohistochemical localization of recombinant human Cu, Zn-SOD in rats after intravenous administration" J. Chin. Biochem. Nutr.18. 171-180 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsuno, A. Watanabe, K. et al: "Changes in the ultrastructural distribution of prolactin and growth hormone mRNAs in pituitary cells of female rats after estrogen and bromocriptine treatment, studied using ISH with biotinylated oligonucleotide probes." Histochem Cell Biol.104. 37-45 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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