• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

ヒトゲノム反復配列の不安定性にもとづく疾患の診断技術の開発

Research Project

Project/Area Number 07557043
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field 内科学一般
Research InstitutionTOKYO INSTITUTE OF TECHNOLOGY

Principal Investigator

ISHIKAWA Fuyuki  Tokyo Institute of Technology Deaprtment of Life Science Professor, 生命理工学部, 助教授 (30184493)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Hideo  Yokohama Research Center, Mitsubishi Chemical Corporation Pharmaceutical Laborat, 横浜総合研究所, 研究員
岡田 典弘  東京工業大学, 生命理工学部, 教授 (60132982)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 1996: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1995: ¥8,000,000 (Direct Cost: ¥8,000,000)
Keywordstelomere / telomerase / stretch PCR assay / leukemia / 反復配列 / PCR
Research Abstract

Telomeres, the ends of chromosomes, are essential components of chromosomes for their stable maintenance.They show a highly dynamic changes, resulted from an equilibrium between the telomere shortening due to cell growth and the telomere elongation due to the telomerase activity. We measured the telomere lengths in normal peripheral blood cells and found that they are shortened as the ages of the donors increased. In leukemic cells, the telomere lengths were invariably shortened compared to those of the normal cells.We developed a novel quantitative stretch PCR assay for the neasurement of the telomerase activity. Telomerase was negarive in most of the normal blood cells. In contrast, it was highly activated in advanced stages of malignancy, such as blastic crisis of CML and relapsed casesof AML.The telomere crisis model was proposed to explain these characteristic features with malignant cells.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Kajita, Y.et al.: "The UUAG-specific RNA binding protein,hnRNP D0:Common modular structure and binding properties of the 2xRBD一Gly family." J.Biol.Chem.270. 22167-22175 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kajita, Y.et al.: "Bioluminescent detection of RNA with sequence-specificity using RNA binding protein-luciferase fusion protein." J.Biotechnol.43. 63-70 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ikeda-Nishizawa, Y.et al.: "Spatial progression of DNA strand breaks in apoptotic HL-60 cells." Bioimages. 4. 63-72 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tatematsu, K.et al.: "A novel quantitative “stretch PCR assay",that detects a dramatic increase in telomerase activity during the progression of myeloid leukemias." Oncogene. 13. 2265-2274 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, F.: "A review article:Telomere crisis in leukemia." Intl.J.Hematol.65. 355-364 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, F.: "A review articIe:Telomere crisis,the driving force in cancer cell evolution." Biochem.Biophys.Res.Commun.230. 1-6 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakayama, J.et al.: "TLP1:A gene encoding a protein component of mammalian telomerase is a novel member of WD-repeats family." Cell. 88. 875-884 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hatakeyama, S.et al.: "ICE:a novel and efficient method for isolation of chromosomal ends." Genetic Analysis:Biomolecular Engineering. 14. 45-46 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, F.: "News and Views:A tale of mice without ‘tails'." Nature genetics. 17. 257-258 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Saito, T.et al.: "Comparative gene mapping of the human and mouse TLP1 genes,that encode one protein component of telomerases." Genomics. 46. 46-50 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, F.: "A review article:Regulation mechanisms of mammalian telomerases." Biochemistry(Moscow). 62. 1332-1337 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakayama, J.et al.: "Telomerase activation by the catalytic component gene TRT in human primary fibroblasts and hepatocellular carcinomas." Nature genetics. 18. 65-68 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hatakeyama, S.et al.: "The jumping translocation at lq21 involves shortened telomeres." Blood. 91. 1514-1519 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kajita, Y., J.Nakamura, M.Aizawa, and F.Ishakawa: "The UUAG-specific RNA binding protein, hnRNP D0 : Common modular structure and binding properties of the 2xRBD-Gly family." J.Biol.Chem.270. 22167-22175 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kajita, Y., E.Kobatake, F.Ishikawa, and M.Aizawa: "Biolujminescent detection of RNA with sequence-specificity using RNA binding proteinluciferase fusion protein." J.Biotechnol.43. 63-70 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ikeda-Nishizawa, Y., Yazaki, Y., Hirai, H.and Ishikawa, F.: "Spatial progression of DNA strand breads in apoptotic HL-60 cells." Bioimages. 4. 63-72 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tatematsu, K., Nakayama, J., Danbara, M., Shionoya, S., Sato, H., Omine, M., and Ishikawa, F.: "A novel quantitative "stretch PCR assay", that detects a dramatic increase in telomerase activity during the progression of myeloid leukemias." Oncogene. 13. 2265-2274 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, F.: "A review article : Telomere crisis in leukemia" Intl.J.Hematol.65. 355-364 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, F.: "A review article : Telomere crisis, the driving force in cancer cell evolution." Biochem.Biophys.Res.Commun.230. 1-6 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakayama, J., Saito, M., Nakamura, H., Matsuura, A., and Ishikawa, F.: "TLP1 : A gene encoding a protein component of mammalian telomerase is a novel member of WD-repeats family." Cell. 88. 875-884 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hatakeyama, S., and Isikawa, F.: "ICE : a novel and efficient method for isolation of chromosomal ends." Genetric Analysis : Biomolecular Engineering. 14. 45-46 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, F.: "News and Views : A tale of mice without 'tails'." Nature genetics. 17. 257-258 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Saito, T., Matsuda, Y., Suzuki, T., Hayashi, A., Yuan, X., Saito, M., Nakayama, J., Hori, T., and Ishikawa, F.: "Comparative gene mapping of the human and mouse TLP1 genes, that encode one protein component of telomerases." Genomics. 46. 46-50 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, F.: "A review artcle : Regulation mechanisms of mammalian telomerases." Biochemistry( Moscow ). 62. 1332-1337 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakayama, J., Tahara, H., Tahara, E., Saito, M., Ito, K., Nakamura, H., Nakanishi, T., Tahara, E., Ide, T.and Ishikawa, F.: "Telomerase activation by the catalytic component gene TRT in human primary fibroblasts and hepatocellular carcinomas." Nature genetics. 18. 65-68 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hatakeyama, S., Fujita, K., Omine, M.and Ishikawa, F.: "The jumping translocation at 1q21 involves shortened telomeres." Blood. 91. 1514-1519 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakayama,J.: "TLP1:A gene encoding a protein component of mammalian telomerase is a novel member of WD-repeats family." Cell. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishikawa,F.: "Areview article:Telomere crisis in leukemia." Intl.J.Hematol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishikawa,F.: "Areview article:Telomere crisis, the driving force in cancer cell evolution." Biochem.Biophys.Res.Commun.230. 1-6 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tatematsu,K.: "A novel quantitative “stretch PCR assay",that detects a dramatic increase in telomerase activity during the progression of myeloid leukemias." Oncogene. 13. 2265-2274 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kajita,Y.,Nakayama,J.,Aizawa,M.,and Ishakawa,F.: "The UUAG-specific RNA binding protein,hnRNP D0:Common modular structure and binding properties of 2xRBD-Gly family" J.Biol.Chem.270. 22167-22176 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kajita,Y.,Kobatake,E.,Ishikawa,F.,and Aizawa,M.: "Bioluminescent detection of RNA with sequence-specificity using RNA binding protein-luciferase fusion protein" J.Biotechnol.43. 63-70 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi