| Project/Area Number |
07557048
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | KUMAMOTO UNIVERSITY |
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Principal Investigator |
ANDO Masayuki Kumamoto Univ.Sch.of Med., Professor, 医学部, 教授 (00040204)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASAKI Hisato Kumamoto Univ.Sch.of Med., Assistance., 医学部・附属病院, 助手 (00271130)
SUGA Moritaka Kumamoto Univ.Sch.of Med., Associate Professor, 医学部, 助教授 (20154437)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥8,100,000 (Direct Cost: ¥8,100,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | Summer-type hypersensitivity pneumonitis / Trichosporon / Polysaccharide antigen / Monoclonal antibody / ELISA method / Serological diagnosis / 単クローン抗体 |
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| Research Abstract |
Summer-type hypersensitivity pneumonitis (S-HP) caused by Trichosporon is the most prevalent type of hypersensitivity pneumonitis in Japan, and recently it is also discovered in Korea. In order to facilitate and simplify the diagnosis of S-HP,we attempt to establish sandwich-ELISA system which enables evaluation of specific antibody activity in serum. We obtained the following results.
1. Purification of monoclonal antibody (Mab) specific for causative antigenisity was done from ascites of mice, and this Mab can work well as capture antibody.
2. Purification of Trichosporon species specific antigen by monoclonal antibody coupled affinity column was done. By using this affinity column, we can obtain an enough amount of antigen to make sandwich-ELISA system.
3. We compared with sandwich-ELISA system and ELISA system in which Trichosporon species specific antigen, glucuronoxylomannan, was chemically fixed to ELISA plate. After measured samples, the former system yielded higher sensitivity and time saving.
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