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Development of in vitro reconstituted system for investigating intracellular signal trasduction and cellular function in myocardial cells

Research Project

Project/Area Number 07557057
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Circulatory organs internal medicine
Research InstitutionOsaka University

Principal Investigator

HORI Masatsugu  Osaka University, Medical School, Professor, 医学部, 教授 (20124779)

Co-Investigator(Kenkyū-buntansha) FUJIWARA Keigi  National Cardiovascular Center Superintendent, 所長 (10190092)
KORETSUNE Yukihiro  Osaka University, Medical Schooll, Assistant Professor, 医学部, 助手 (50243217)
KURIHARA Toshinao  Osaka University, Medical School Hospital, Medical Staff, 医学部・附属病院, 医員
YONEDA Yoshihiro  Osaka University, Medical School, Professor, 医学部, 教授 (80191667)
TADA Michihiko  Osaka University, Medical School, Professor, 医学部, 教授 (90093434)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 1996: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1995: ¥12,000,000 (Direct Cost: ¥12,000,000)
Keywordsintracellular signal transduction / nuclear localization signal / myocardial cell / cellular biology / 心筋 / 細胞機能
Research Abstract

The aim of this research is to develop an in vitro real-time analyzing system for investigating intracellular signal transduction with CCD camera. In 1995, we succeeded to develop an in vitro reconstituted system for analyzing of intracellular signal transduction, especially nuclear signal transduction. It was found that nuclear protein was transported to the nucleus within 30 minutes, although non-nuclear proteins were not by using homogenates from heart which means that this system reproduces in vivo nuclear protein transport. In 1996, we examined nuclear transport efficiency with extracts from diseased hearts by this in vitro system. The rate of the nuclear transport was more decreased in spontaneous hypertensive rat than of the control. Furthermore, we found the existence of cellular factors that are activated by nuclear localization signal (NLS) and partially purified these cellular factors (FEBS Letters). In the next step, we should improve this system in order to analyze the real-time behavior of molecules quantitatively with CCD camera. The real-time analytical system will provide the precise evaluation of efficiency of nuclear transport that may modulate the cellular function in heart cells.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Yoneda Y. et al.: "Molecular mechanizm of nucleocytoplasmic transport of proteins through the nuclear membrane." International symposium MEMBRANCE PROTEINS structure, function and expression contorol.9-16 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sekimot T. et al.: "Interferon-γ-dependent nuclear import of Stat 1 is mediated by the GTP ase activity of Ran/TC4." J Biol Chem. 271. 31017-31020 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoneda Y. et al.: "Nuclear export and its significance in retroviral infection." Trends in Microbiology. 4. 1-2 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoneda Y. et al.: "Nuclear pore-targetting complex and its role on nuclear protein transport." Arch Histol Cytol. 59. 97-107 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tachibana T. et al.: "Exogenously injected nuclear import factor p10/NTF2 inhibits signal-mediated nuclear import and export of proteins in living cells." FEBS Letters. 397. 177-182 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurihara T. et al.: "Partial purification and charatcrization of a protein protein kinase that is activated by nuclear localization signal peptides." FEBS Letters. 380. 241-245 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Imamoto N. et al.: "In vivo evidence for involvement of a 58 kDa component if nuclear poretargeting complex in nuclear protein import." EMBO-J. 14. 3617-3626 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Imamoto N. et al.: "A karyophilic protein forms a stable complex binds to nuclear pores after" FEBS-Letters. 368. 415-419 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Imamoto N. et al.: "A karyophilic protein forms a stable complex with cytoplasmic components prior to nuclear pore binding." J Biol Chem.270. 8559-85565 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Terada K. et al.: "Role of hear shock cognate 70 protein in import of ornithine transcarbamylase precursor into mammalian mitochondria." Mol Cell Biol. 15. 3708-3713 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoneda, Y,Imamoto N.et al.: "Molecular mechanizm of nucleocytoplasmic transport of proteins through the nuclear membrane." International symposium MEMBRANCE PROTEINS structure, function and expression control. 9-16 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sekimoto T,Yoneda Y.et al.: "Interferon-gamma-dependent nuclear import of Stat 1 is mediated by the GTPase activity of Ran/TC4." J Biol Chem. 271. 31017-31020 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoneda Y.: "Nuclear export and its significance in retroviral infection." Trends in Microbiology. 4. 1-2 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoneda Y.: "Nuclear pore-targetting complex and its role on nuclear protein transport." Arch Histol Cytol. 59. 97-107 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tachibana T,Yoneda Y.et al.: "Exogenously injected nuclear import factor p10/NTF2 inhibits signal-mediated nuclear import and export of proteins in living cells." FEBS Letters. 397. 177-182 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurihara T.et al.: "Partial purification and charaterization of a protein protein kinase that is activated by nuclear localization signal peptides." FEBS Letters. 380. 241-245 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Imamoto N,Yoneda Y.et al.: "In vivo evidence for involvement of a 58 kDa component if nuclear poretargeting complex in nuclear protein import." EMBO-J. 14. 3617-3626 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Imamoto N,Yoneda Y.et al.: "The nuclear pore-targeting complex binds to nuclear pores after association with a karyophile." FEBS-Letters. 368. 415-419 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Imamoto N,Yoneda Y.et al.: "A karyophilic protein forms a stable complex with cytoplasmic components prior to nuclear pore binding." J Biol Chem. 270. 8559-85565 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Terada K,Yoneda Y.et al.: "Role of heat shock cognate 70 protein in import of ornithine transcarbamylase precursor into mammalian mitochondria." Mol Cell Biol. 15. 3708-3713 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Toshinao Kurihara: "Partial purification and characterization of a protein kinase that is activated by nuclear localization signal peptides" FEBS Letters. 380. 241-245 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toshinao Kurihara et al.: "Partial purification and characterization of a protein kinase that is activated by nuclear localization signal peptides" FEBS letter. 380. 241-245 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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