| Project/Area Number |
07557063
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Pediatrics
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| Research Institution | Kitasato University |
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Principal Investigator |
MATUURA Nobuo Kitasato Univ.School of Medicine, Professor, 医学部, 教授 (50002332)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAYAMA Yoshinaga Kitasato Univ.School of Medicine, Reserch Associate, 医学部, 助手 (90245407)
TAKAGAKI Yotaro Kitasato Univ.School of Medicine, Professor, 医学部, 教授 (50281324)
TAKADA Fumio Kitasato Univ.School of Medicine, Reserch Associate, 医学部, 助手 (90206764)
OGUCHI Kouki Kitasato Univ.School of Medicine, Assistant Professor, 医学部, 講師 (30133292)
KOBAYASHI Kunihiko Hokkaindo Univ.School of Medicine, Professor, 医学部, 教授 (60091451)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥10,000,000 (Direct Cost: ¥10,000,000)
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| Keywords | complement / infirmative constitution / recurrent infectious / complement activation / gene analysis / mannan binding lectin (MBL) / Ra-reactive factor (RaRF) / gene diagnosis / Mannan binding protein (MBP) / Ra-reactive factor (RaRF) / Maunose binding protein (MBP) |
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| Research Abstract |
1.Development of a rapid diagnosis system for estimation of serum P100 levels.
Preparation of the P100 specific anti recombinant P100 antibodies : After reproduction of the recombinant proteins that fused with heavy or light chains of P100 and glutathion-S-transferase, the anti-recombinant P100 antibodies were prepared form immunized rabbit serum and assayd those specific activities. To determine of the serum P100 concentrations, we are performing the adjustment for the conditions of the sandwich method using anti-peptid and anti-recombinant antibodies.
2.Development of a rapid diagnosis system for the P100 gene.
Allotype analysis of the P100 gene : The nucleotide sequences of the P100 exons were direct determine from PCR amplified products that genome DNA was prepared from a normal adult peripheral blood lymphocyte as a template. Five allotypic amino acid substitutions derived from nucleotide mutations were identified comparisons with genomic and cDNA nucleotide sequences. Although screen for 300 genome DNA samples using mutation specific PCR primers that closely related to His exon, the mutations has not been find out. To the analysis for the survey of P100 genomic mutation, it is needed not only normal but recurrent infectious patients genome DNA.At the present, we are preparing those of genome DNA samples and searching for the presence of remained nucleotide mutatios. To the next step for the gene diagnosis system of P100, we are planning to screening conditions for the PCR-SSCP gene diagnostic method.
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