| Project/Area Number |
07557065
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Radiation science
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
IDO Tatsuo Tohoku Univ.CYRIC Professor, サイクロトロン・ラジオアイソトープセンター, 教授 (80134063)
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| Co-Investigator(Kenkyū-buntansha) |
HATANO Kentaro National Inst.Longevity Sciences Senior Researcher, 長寿医療研究センター, 主任研究員 (50228475)
FUNAKI Yoshihito Tohoku Univ.CYRIC Research Associate, サイクロトロン・ラジオアイソトープセンター, 助手 (50261491)
IWATA Ren Tohoku Univ.CYRIC Assoceate Professor, サイクロトロン・ラジオアイソトープセンター, 助教授 (60143038)
IMAHORI Yoshio Kyoto Prefect.Univ.Med., Research Associate, 附属病院・脳神経外科, 助手 (80191899)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 1996: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1995: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | Brain function mapping / Positron emission tomography / ^<11>C-diacylglycerol / ^<18>F-diacylglycerol / Second messenger / Radiopharmaceuticals / phosphatidylinsitol / phosphatidylithanolamine / ^<18>Fジアルシルグリセロール |
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| Research Abstract |
The new concept of the analytical method of human brain function by visualizing second messenger systems in cellular signal transduction has been devoloped by PET studies using ^<11>C or ^<18>F labeled 1,2-diacylglycerols. 1-palmitoy1-2[1-^<11>C] butyry1-sn-glycerol and 1-[1-^<11>C]butyry1-2-palmitoy1-rac-glycerol (^<11>C-DAG) were synthesized by reaction of [^<11>C] -ethylketen which was produced by pyrolysis of [1-^<11>C] butyric acid. Both were remarkably incorporated into brain through the BBB and trapped in cell membrane as ^<11>C-phosphatidylinositols. The PET study of human brain under resting conditions revealed higher activity in the neocortical region including the association area. ^<11>C-DAG incorporation which is limited by receptor-mediated PI turnover becomes possible to elicit post signal transduction in the human brain. 1-(8-[^<18>F]fluorooctanoy1) -2-palmitoy1-rac-glycerol was synthesized by the reaction of 8-[^<18>F]fluorooctanoy1 chloride and 2-palmitoylglycerol. This copound was also incorporated in rat brain and tumor tissue and trapped into cell as ^<18>F-phosphatidy1-ethanolamine. This characteristic metabolic pathway of ^<18>F-FDAG becomes possible to realize the delayd signal transduction related to long termed memory and cell proliferation.
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