| Project/Area Number |
07557096
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NODA Masaki Tokyo Medical and Dental University, Medical Research Institute, professor, 難治疾患研究所, 教授 (50231725)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Masato Kagoshima Univ., School of Dent., Assistant Professor, 歯学部, 助手 (30236757)
NIFUJI Akira Tokyo Medical and Dental University, Medical Research institute, Lecturer, 難治疾患研究所, 講師 (00240747)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥20,300,000 (Direct Cost: ¥20,300,000)
Fiscal Year 1997: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1996: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1995: ¥10,900,000 (Direct Cost: ¥10,900,000)
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| Keywords | bone / ligament / osteoporosis / osteoblast / transcription / promoter / type I collagen / transgenic animal / 遺伝子発現 / 靭帯 / BMP / 骨形成 / スクリーニング系 / 移植 / ベータガラクトシダーゼ / 薬剤スクリーニング / 転写因子 / トランスジェニックマウス / ベータガラトクシダーゼ / osteoblast / transcription / osteoclast / cytokine / osteoporosis / differention |
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| Research Abstract |
We generated chloromphenicol acetyl transferase reporter construct using 1kb promoter region of rat osteocalcin gene and analyzed the transcriptional activity. Collagen (type I) is a major bone protein which comprises about 90% of the matrix activity in vivo. Betagalactosidase reporter construct was used for this collagen promoter study. Both osteocalcin and collagen promoter activity was confirmed in in vivo experiments. Furthemore, bone-forming agents, such as TGFbeta and BMP were found to be able to stimulate production of collagen and activation of the promoters. The transgenic animals containing collagen promoter fragment are useful for studying bone-related agents.
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