| Project/Area Number |
07557105
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Urology
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| Research Institution | Kochi Medical School |
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Principal Investigator |
SHUIN Taro Kochi Medical School, Department of urology Professor, 医学部, 教授 (80179019)
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| Co-Investigator(Kenkyū-buntansha) |
YAO Masahiro Yokohama City University, School of medicine Department of urology Instructor, 医学部, 講師 (00260787)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1995: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | Von Hippel-Lindau (VHL) disease / VHL tumor suppressor gene / transfection / Herpes Simplex virus vector ; Gene therapy / von Hippel-Lindau病遺伝子 / HSVウイルス |
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| Research Abstract |
Von Hippel-Lindau (VHL) disease is a multitumor syndrome which shows hemangioma of the retina, hemangiblastoma of the central nervous system, pheochromocytoma of the adrenal gland, and renal cell carcinoma of the kidney as major tumors. The responsible gene for this disease, the VHL tumor suppressor gene was identified at chromosome 3p25-26 in 1993. To make a good model system for the introduction of VHL gene into human cells, we examined the efficacy of Herpes Simplex virus (HSV) vector, which are usually used to express foreign genes in cells in the central nervous system (CNS), for human renal cell carcinoma cell. VHL gene was efficiently introduced and expressed in renal cell carcinoma cell lines and other CNS cancer cells with HSV vector. Introduced VHL gene is localized in the cytoplasm. The cell introduced with VHL gene showed growth inhibitory effect at shorter incubation period. However, HSV vector itself has some cytotoxic effects to the introduced kidney cancer cells after long incubation with HSV vector.
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