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Molecular analysis of epithelial mesenchymal interaction during tooth formatio

Research Project

Project/Area Number 07557112
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Morphological basic dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

NIFUJI Akira (1997)  Tokyo Medical and Dental University Medical, Research Institute, Lecturer, 難治疾患研究所, 講師 (00240747)

田村 正人 (1995-1996)  東京医科歯科大学, 難治疾患研究所, 助手 (30236757)

Co-Investigator(Kenkyū-buntansha) TAMURA Masato  Kagoshima Univ., School of Dent., Assistant Professor, 歯学部, 助手 (30236757)
NODA Masaki  Tokyo Medical and Dental University Medical, Research Institure, Professor, 難治疾患研究所, 教授 (50231725)
二藤 彰  東京医科歯科大学, 難治疾患研究所, 助手 (00240747)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥17,700,000 (Direct Cost: ¥17,700,000)
Fiscal Year 1997: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1996: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1995: ¥9,900,000 (Direct Cost: ¥9,900,000)
Keywordssignal / epithelium / mesenchyme / subtraction / amelogenin / noggin / in situ hybridization / BMP / 幹細胞様細胞株C1 / スクリーニング / 歯胚 / 歯 / 上皮・間葉相互作用 / 遺伝子発現 / 分化
Research Abstract

Embryonic mouse tooth germs were excised and were devided into epithelium and mesenchyme followed by combination cultures to examine gene expression during tooth development and formation. Complementary DNA library was made using RNAs prepared from these combinatorial cultures. We cloned novel genes expressed during tooth development. We also analyzed 6kb amelogenin gene promoter fragment. Using another cDNA library made from C1 cell RNA,we cloned out noggin cDNA.In situ hybridization revealed novel expression pattern of noggin during hard tissue formation and development. These molecules would play their role as signals during embryogenesis.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] M.Tamura et al.: "Interleukin-1a stimulates interstital collagenase gene expression in human dental pulp fibroblasts in culture." Journal of Endodonitics. 22. 240-243 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Nagaoka, M.Tamura et al.: "Interleukin-8 gene expression by human dental pulp fibrobiast in cultures stimulated with Prevotella intermedia lipopolysaccharide." Journal of Endodonitics. 22. 9-12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Nagaoka, M.Tamura et al.: "Β-adrenergic receptor kinase mRNA expression in human dental pulp." Journal of Endodonitics Association. 17. 162-166 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shionoya, M.Tamura et al.: "Relationship between transterin receptor expression and proliferating acti vity of tumor cells." Asian Journal of Oral and Maxilofacial Surger. 8. 73-78 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Mataga, M.Tamura et al.: "Establishment of a Novel Chondrocyte-like Cell Line Derived from Transgenic Mice Harboring theTemperature-Sensitive Simian Virus 40 Large T-Antigen Gene" Journal of Bone and Mineral Research. 11. 1646-1654 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamura et al.: "1α,25-dihydroxyvitamin D_3 down-regulates pleiotrophin messenger RNA expression in osteoblase-like cells." Endocrine. 3. 21-24 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Tamura, S.Nagaoka and M.Kawagoe: "Interleukin-1a stimulates interstitial collagenase gene expression in human dental pulp fibroblasts in culture." Journal of Endodontics. 22. 240-243 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Nagaoka, M.Tokuda, T.Sakuta, Y.Taketoshi, M.Tamura, H.Takada and M.Kawagoe: "Interleukin-8 gene expression by human dental pulp fibroblast in cultures stimulated with Prevotella intermedia lipopolysaccharide." Journal of Endodontics. 22. 9-12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Nagaoka, M.Kawagoe, M.Tamura, M.Obinata, D.T.Denhardt and M.Tokuda: "beta-adrenergic receptor kinase mRNA expression in human dental pulp." Jornal of Japan Endodontics Association. 17. 162-166 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shionoya, N.Tanaka, T.Miyamoto, T.Amagasa, M.Tamura and Y.Eichi: "Relationship between transferin receptor expression and proliferating acti vity of tumor cells." Asian Journal of Oral and Maxilofacial Surger. 8. 73-78 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Mataga, M.Tamura, N.Yanai, T.Shinomura, K.Kimata, M.Obinata and M.Noda: "Establishment of a Novel Chondrocyte-like Cell Line Derived from Transgenic Mice Harboring the Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene" Journal of Bone and Mineral Research. 11. 1646-1654 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Tamura, F.Ichikawa, R.P.Guillerman, T.F.Deuel and M.Noda: "1 _<alpha>,25-dihydroxyvitamin D_3 down-regulates pleiotrophin messenger RNA expression in osteoblast-like cells." Endocrine. 3. 21-24 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masato Tomura: "Interleukin-1α stimulates interstitial collagenase gene expression in human dental pulp fibroblasts in culture" Journal of Endodontics. 22. 240-243 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shigetaka Nagaoka: "Interleukin-8 gene expression by human dental pulp fibroblasts in cultures stimulated with prevotella intermedia Lipopolysaccharide" Journal of Endodontics. 22. 9-12 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shigetaka Nagaoka: "β-adrenergic receptor kinase mRNA expression in human dental pulp" Journal of Japan Endodontics Association. 17. 162-166 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kinichi Shionoya: "Relationship between transferin receptor expression and proliferating activity of tumor cells" Asian Journal of Oral and Maxilofacial Surgery. 8. 73-78 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nobuko Mataga: "Establishment of a Novel Chondrocyte-Like Cell Line derived from transgenic mouse harboring the temperature-sensitive simian virus 40 Large T antigen" Journal of Bone and MIneral Research. 11. 1646-1654 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田村正人: "1α,25-dihydroxyvitamin D3 down-regulated pleiotrophin messenger RNA expression in osteoblast-Like cells" Endocrine. 3. 21-24 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 田村正人: "Interleukin-1α stimulates interstitial collagenase gene expression in human dental pulp fibroblast" Journal of Endodontics. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 長岡成孝: "Interleukin-8 gene expression by human dental pulp fibroblast in cultures stimulated with Prerotella intermedia Lypopolysaccaride" Journal of Endodontics. 22. 9-12 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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