| Project/Area Number |
07557112
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NIFUJI Akira (1997) Tokyo Medical and Dental University Medical, Research Institute, Lecturer, 難治疾患研究所, 講師 (00240747)
田村 正人 (1995-1996) 東京医科歯科大学, 難治疾患研究所, 助手 (30236757)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Masato Kagoshima Univ., School of Dent., Assistant Professor, 歯学部, 助手 (30236757)
NODA Masaki Tokyo Medical and Dental University Medical, Research Institure, Professor, 難治疾患研究所, 教授 (50231725)
二藤 彰 東京医科歯科大学, 難治疾患研究所, 助手 (00240747)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥17,700,000 (Direct Cost: ¥17,700,000)
Fiscal Year 1997: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1996: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1995: ¥9,900,000 (Direct Cost: ¥9,900,000)
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| Keywords | signal / epithelium / mesenchyme / subtraction / amelogenin / noggin / in situ hybridization / BMP / 幹細胞様細胞株C1 / スクリーニング / 歯胚 / 歯 / 上皮・間葉相互作用 / 遺伝子発現 / 分化 |
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| Research Abstract |
Embryonic mouse tooth germs were excised and were devided into epithelium and mesenchyme followed by combination cultures to examine gene expression during tooth development and formation. Complementary DNA library was made using RNAs prepared from these combinatorial cultures. We cloned novel genes expressed during tooth development. We also analyzed 6kb amelogenin gene promoter fragment. Using another cDNA library made from C1 cell RNA,we cloned out noggin cDNA.In situ hybridization revealed novel expression pattern of noggin during hard tissue formation and development. These molecules would play their role as signals during embryogenesis.
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