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BIOLOGICAL CONTROL OF OSTEOCLASTS AND OSTEOBLASTS

Research Project

Project/Area Number 07557133
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field 矯正・小児・社会系歯学
Research InstitutionTHE UNIVERSITY OF TOKUSHIMA

Principal Investigator

SUMITANI Koji  THE TOKUSHIMA UNIVERSITY,SCHOOL OF DENTISTRY,ASSISTANT PROFESSOR, 歯学部・附属病院, 講師 (30206586)

Co-Investigator(Kenkyū-buntansha) HIURA Kenji  THE TOKUSHIMA UNIVERSITY,SCHOOL OF DENTISTRY,ASSISTANT PROFESSOR, 歯学部・附属病院, 講師 (20228696)
上岡 寛  徳島大学, 歯学部, 助手 (80253219)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥6,000,000 (Direct Cost: ¥6,000,000)
Keywordsosteoclast (s) / osteoblast (s) / bone resorption / MC3T3-E1 / カテプシンL / プロトンポンプ / carbonic anhydrase II
Research Abstract

In this study, we examined the biological controls of osteoblasts to osteoclasts in the activation and formation. To examine them, we used a culture system as follows,
(1) unfractionated cells obtained from mouse long bones for osteoclastic formation
(2) stromal cell free hematopoietic blast cells (mouse) for multinucleated cell formation
(3) cloned mouse osteoblastic cells (MC3T3-E1)
The results were obtained as follows,
1) The conditioned medium (CM) of MC3T3-E1 cells precultured for 3days contained only the inhibitory factor of multinucleated cell formation. On the other hand, both inhibitory factor and stimulatory factor were found in the CM of MC3T3-E1 cells precultured for 60days. No such effectors were found in the CM of fibroblasts.
2) The production of inhibitory and stimulatory factors were depressed by indomethacin and cycloheximide, respectively.
3) The media of MC3T3-E1 cells precultured for 3 and 60days contained prostaglandin E2 which inhibited multinucleated cell formation.
4) … More 1alpha, 25 (OH)_2D_3 was essential for stimulation of MNC formation by both MC3T3-E1 cells derived stimulatory factor and GM-CSF.
These results suggest that the inhibitory factor is prostaglandin E2 and the stimulatory factor is GM-CSF like protein.
5) Although TGF-beta1 has potent inhibitory effect on osteoclastic bone resorption, in the presence of 1alpha, 25 (OH)_2D_3, TGF-beta1 stimulated the formation of osteoclast.
6) TGF-beta1 inhibited multinucleated cell formation induced by 1alpha, 25 (OH)_2D_3, but the conditioned medium of TGF-beta1-treated MC3T3-E1 cells stimulated such formation.
7) Both bafilomycin A_1 (H^+-ATPase inhibitor) and acetazolamide (carbonic anhydorase II inhibitor) inhibited osteoclastic bone resorption stimulated by parathyroid hormone.
On the other hand, bafilomycin A1 did not affect procathepsin L secretion while acetazolamide inhibited the secretion.
These findings suggest that osteoblasts have the effect on osteoclastic formation through calcium regulating factors such as prostaglandin E2, GM-CSF and TGF-beta1. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Kakegawa.H: "Sectretion and processing mechanisms of procathepsin L in bone resorption" FEBS Lett.370(1,2). 78-82 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kamioka.H: "Extracellular calcium causes the release of calcium from in tracellular stores in check osteocytes" Biochem.Biophys Res Commun.212(2). 692-696 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohba.Y: "Inhibitory mechanisms of H^t-ATP ase inhibitor bafilomycin A1 and carbonic anhydrase II inhibitor acetazolamide on experimetal bone resorption" FEBS Lett.387(2,3). 175-178 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 高田 幸宏: "骨形成と骨吸収及びそれらの調節因子 2巻" 廣川 節男, 494 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kakegawa-H ; Tagami-K ; Ohba-Y ; Sumitani-K ; Kawata-T ; Katunuma-N: "Secretion and processing mechanisms of procathepsin L in bone resorption" FEBS-Lett. 370. 78-82 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kamioka-H ; Miki-Y ; Sumitani-K ; Tagami-K ; Terai-K ; Hosoi-K ; Kawata-T: "Extracellular calcium causes the release of calcium from intracellular stores in chick osteocytes" Biochem-Biophys-Res-Commun. 212. 692-696 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohba-Y ; Ohba-T ; Sumitani-K ; Tagami-Kondoh-K ; Hiura-K ; Miki-Y ; Kakegawa-H ; Takano-Yamamoto-T ; Katunuma-N: "Inhibitory mechanisms of H (+) -ATPase inhibitor bafilomycin A1 and carbonic anhydrase II inhibitor acetazolamide on experimental bone resorption" FEBS-Lett. 387. 175-178 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kakegawa,H: "Secretion and Processing mechanisms of procathepsin L in bone rosorption" FEBS Lett.370(1,2). 78-82 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kamioka,H: "Extracellular calcium causes the release of calcium from intracellula stores in chick osteocytes" Biochem Biophys Res Commun. 212(2). 692-696 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohba,Y: "Inhibitroy mechanisms of H^+ATP ase inhibitor bafllomycin Al and Carbonic anhydrase II inhibitor acetwolamide on experimental bone resorptia" FEBS Lett.387(2,3). 175-178 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高田 幸宏: "骨形成と骨吸収及びそれらの調節因子 2巻" 廣川節男, 494 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hisao Kakegawa: "Sectretion and processing mechanisms of prcathepsih L in bone resorption" FEBS Letters. 370. 78-82 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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